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Last modified by chatzies on 2025-11-27 11:35
From version 11.1
edited by ahenkel
on 2021-12-01 11:31
Change comment: There is no comment for this version
To version 21.1
edited by ahenkel
on 2021-12-03 11:18
Change comment: There is no comment for this version

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... ... @@ -58,20 +58,99 @@
58 58  
59 59  * filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
60 60  
61 +[[image:attach:20211201_095534.jpg||height="250"]]
62 +
61 61  \\
62 62  
65 +**14.3 Connecting sample to ElveFlow, sample exchange
66 +**
67 +
68 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bttom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
69 +
70 +Be aware to which channel and nozzle line your sample is connected.
71 +
72 +Red → Channel 1, crystal line (nozzle capillary without marks)
73 +
74 +Green → Channel 2, substrate line (nozzle capillary is marked in black)
75 +
76 +[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
77 +
63 63  \\
64 64  
65 -**14.3 Connecting sample to ElveFlow**
80 +\\
66 66  
82 +**14.4 TapeDrive operation
83 +**
84 +
85 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
86 +Now start the client. Same thing, a terminal will open, shortly after a small window.
87 +
88 +Click 'Devices' in the **client session** to open the control window.
89 +[[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
90 +
91 +* **Tape:**
92 +** Click on the 'main tape' icon
93 +** Change the velocity by typing anything from **0.3-3.0 mm/s**
94 +** Common velocity is 1.0-1.5 mm/s
95 +** kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
67 67  \\
97 +** [[image:attach:kamzik_tape.PNG||height="400"]]
68 68  
69 69  \\
70 70  
101 +* **Sample nozzle:**
102 +** Click an the 'nozzle' icon.
103 +** (((
104 +The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
105 +The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
106 +\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
107 +\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
108 +
109 +(% class="wrapped" %)
110 +|=(((
71 71  \\
112 +)))|=(((
113 +direction
114 +)))|=(((
115 +recommended step size
116 +for visual adjustment
117 +)))|=(% colspan="1" %)(((
118 +recommended step size
119 +during data collection
120 +)))
121 +|(((
122 ++x
123 +)))|(((
124 +away from X-ray interaction point
125 +)))|(((
126 +1.0 - 0.5 mm
127 +)))|(% colspan="1" %)(((
128 +0.1 - 0.2 mm
129 +)))
130 +|(((
131 ++y
132 +)))|(((
133 +up
134 +)))|(((
135 +1.0 - 0.5 mm
136 +)))|(% colspan="1" %)(((
137 +0.1 - 0.2 mm
138 +)))
139 +|(((
140 ++z
141 +)))|(((
142 +towards detector tower, away from tape
143 +)))|(((
144 +0.5 - 0.2 mm
145 +)))|(% colspan="1" %)(((
146 +0.1 - 0.2 mm
147 +be careful with -z, tape could be pushed out of focus
148 +)))
72 72  
73 -**14.4 TapeDrive operation
74 74  **
151 +\\**
152 +)))
153 +** [[image:attach:kamzik_nozzle.PNG||height="400"]]
75 75  
76 76  \\
77 77  
... ... @@ -79,6 +79,35 @@
79 79  
80 80  \\
81 81  
161 +Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
162 +
163 + xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
164 + password: //written on control laptop (changes every 6 months)
165 +//
166 +
82 82  \\
83 83  
84 84  \\
170 +
171 +**14.5 Tape roll exchange**
172 +
173 +The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
174 +
175 +* Remove the (almost) empty feeder roll by pulling it upwards.
176 +* Cut the tape with some cm overlap to glue to the new roll.
177 +* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
178 +* Cut the first cm of the new tape roll, directly before the black sticky part.
179 +* Use this sticky part to attach the end of the old
180 +
181 +**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
182 +**
183 +
184 +\\
185 +
186 +\\
187 +
188 +\\
189 +
190 +\\
191 +
192 +\\
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235034364
1 +235039450
URL
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1 -https://confluence.desy.de/spaces/11/pages/235034364/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235039450/14. Serial Crystallography: CFEL TapeDrive