Skip to Content
Last modified by chatzies on 2025-11-27 11:35
From version 12.1
edited by ahenkel
on 2021-12-01 11:37
Change comment: There is no comment for this version
To version 23.1
edited by ahenkel
on 2021-12-03 11:32
Change comment: There is no comment for this version

Summary

Details

Page properties
Content
... ... @@ -1,7 +1,13 @@
1 1  [[image:attach:TapeDrive_beschriftet.PNG||height="400"]][[image:attach:TapeDrive scheme.png||height="400"]]
2 2  
3 -**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
3 +\\
4 4  
5 +\\
6 +
7 +\\
8 +
9 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
10 +
5 5  * remove vortex
6 6  * beamstop to out-position?
7 7  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
... ... @@ -50,7 +50,7 @@
50 50  
51 51  \\
52 52  
53 -**14.2 Sample characteristics and preparation**
59 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
54 54  
55 55  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
56 56  
... ... @@ -62,32 +62,140 @@
62 62  
63 63  \\
64 64  
65 -**14.3 Connecting sample to ElveFlow, sample exchange
66 -**
71 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
72 +** ===
67 67  
68 -[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]][[image:attach:20211201_095205.jpg||height="250"]][[image:attach:20211201_095232.jpg||height="250"]]
74 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
69 69  
76 +Be aware to which channel and nozzle line your sample is connected.
77 +
78 + Red → Channel 1, crystal line (nozzle capillary without marks)
79 +
80 + Green → Channel 2, substrate line (nozzle capillary is marked in black)
81 +
82 +[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
83 +
70 70  \\
71 71  
72 72  \\
73 73  
74 -**14.4 TapeDrive operation
88 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
89 +** ===
90 +
91 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
92 +Now start the client. Same thing, a terminal will open, shortly after a small window.
93 +
94 +Click 'Devices' in the **client session** to open the control window.
95 +[[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
96 +
97 +**Tape**
98 +
99 +* Click on the 'main tape' icon
100 +* Change the velocity by typing anything from **0.3-3.0 mm/s**
101 +* Common velocity is 1.0-1.5 mm/s
102 +* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
103 +\\
104 +* [[image:attach:kamzik_tape.PNG||height="400"]]
105 +
106 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle:"/}}**Sample nozzle:** ====
107 +
108 +* Click an the 'nozzle' icon.
109 +* (((
110 +The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
111 +The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
112 +\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
113 +\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
114 +
115 +(% class="wrapped" %)
116 +|=(((
117 +\\
118 +)))|=(((
119 +direction
120 +)))|=(((
121 +recommended step size
122 +for visual adjustment
123 +)))|=(% colspan="1" %)(((
124 +recommended step size
125 +during data collection
126 +)))
127 +|(((
128 ++x
129 +)))|(((
130 +away from X-ray interaction point
131 +)))|(((
132 +1.0 - 0.5 mm
133 +)))|(% colspan="1" %)(((
134 +0.1 - 0.2 mm
135 +)))
136 +|(((
137 ++y
138 +)))|(((
139 +up
140 +)))|(((
141 +1.0 - 0.5 mm
142 +)))|(% colspan="1" %)(((
143 +0.1 - 0.2 mm
144 +)))
145 +|(((
146 ++z
147 +)))|(((
148 +towards detector tower, away from tape
149 +)))|(((
150 +0.5 - 0.2 mm
151 +)))|(% colspan="1" %)(((
152 +0.1 - 0.2 mm
153 +be careful with -z, tape could be pushed out of focus
154 +)))
155 +
75 75  **
157 +\\**
158 +)))
159 +* [[image:attach:kamzik_nozzle.PNG||height="400"]]
76 76  
161 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
162 +
77 77  \\
78 78  
79 79  \\
80 80  
81 -**14.5 Tape roll exchange**
167 +\\
82 82  
83 -**[[image:attach:20211201_094111.jpg||height="250"]][[image:attach:20211201_094101.jpg||height="250"]]**
169 +\\
84 84  
171 +Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
172 +
173 + xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
174 + password: //written on control laptop (changes every 6 months)
175 +//
176 +
85 85  \\
86 86  
87 87  \\
88 88  
181 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ====
182 +
183 +The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
184 +
185 +* Remove the (almost) empty feeder roll by pulling it upwards.
186 +* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
187 +* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
188 +* Cut the first cm of the new tape roll, directly before the black sticky part.
189 +* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
190 +* Open magnetic tape stabilizer.
191 +* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
192 +* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
193 +* Attach new tape to the empty roll by using the black sticky part.
194 +* Close tape stabilizer.
195 +
196 +**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
197 +**
198 +
89 89  \\
90 90  
91 91  \\
92 92  
93 93  \\
204 +
205 +\\
206 +
207 +\\
Confluence.Code.ConfluencePageClass[0]
Id
... ... @@ -1,1 +1,1 @@
1 -235035530
1 +235039462
URL
... ... @@ -1,1 +1,1 @@
1 -https://confluence.desy.de/spaces/11/pages/235035530/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235039462/14. Serial Crystallography: CFEL TapeDrive