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Last modified by chatzies on 2025-11-27 11:35
From version 16.1
edited by ahenkel
on 2021-12-02 13:31
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To version 25.1
edited by ahenkel
on 2021-12-03 11:34
Change comment: There is no comment for this version

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1 -[[image:attach:TapeDrive_beschriftet.PNG||height="400"]][[image:attach:TapeDrive scheme.png||height="400"]]
2 2  
3 -**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
4 4  
3 +{{toc/}}
4 +
5 +[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
6 +
7 +\\
8 +
9 +\\
10 +
11 +\\
12 +
13 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
14 +
5 5  * remove vortex
6 6  * beamstop to out-position?
7 7  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
... ... @@ -50,7 +50,7 @@
50 50  
51 51  \\
52 52  
53 -**14.2 Sample characteristics and preparation**
63 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
54 54  
55 55  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
56 56  
... ... @@ -62,36 +62,106 @@
62 62  
63 63  \\
64 64  
65 -**14.3 Connecting sample to ElveFlow, sample exchange
66 -**
75 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
76 +** ===
67 67  
68 -[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]][[image:attach:20211201_095205.jpg||height="250"]][[image:attach:20211201_095232.jpg||height="250"]]
78 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
69 69  
80 +Be aware to which channel and nozzle line your sample is connected.
81 +
82 + Red → Channel 1, crystal line (nozzle capillary without marks)
83 +
84 + Green → Channel 2, substrate line (nozzle capillary is marked in black)
85 +
86 +[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
87 +
70 70  \\
71 71  
72 72  \\
73 73  
74 -**14.4 TapeDrive operation
75 -**
92 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
93 +** ===
76 76  
77 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open, shortly after a small window.
95 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
78 78  Now start the client. Same thing, a terminal will open, shortly after a small window.
79 79  
80 80  Click 'Devices' in the **client session** to open the control window.
81 81  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
82 82  
83 -* **Tape:**
84 -** Click on the 'main tape' icon
85 -** Change the velocity by typing anything from 0.3-3.0 mm/s
86 -** Common velocity is 1.0-1.5 mm/s
87 -** [[image:attach:kamzik_tape.PNG||height="400"]]
101 +**Tape**
88 88  
103 +* Click on the 'main tape' icon
104 +* Change the velocity by typing anything from **0.3-3.0 mm/s**
105 +* Common velocity is 1.0-1.5 mm/s
106 +* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
89 89  \\
108 +* [[image:attach:kamzik_tape.PNG||height="400"]]
90 90  
110 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle:"/}}**Sample nozzle:** ====
111 +
112 +* Click an the 'nozzle' icon.
113 +* (((
114 +The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
115 +The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
116 +\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
117 +\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
118 +
119 +(% class="wrapped" %)
120 +|=(((
91 91  \\
122 +)))|=(((
123 +direction
124 +)))|=(((
125 +recommended step size
126 +for visual adjustment
127 +)))|=(% colspan="1" %)(((
128 +recommended step size
129 +during data collection
130 +)))
131 +|(((
132 ++x
133 +)))|(((
134 +away from X-ray interaction point
135 +)))|(((
136 +1.0 - 0.5 mm
137 +)))|(% colspan="1" %)(((
138 +0.1 - 0.2 mm
139 +)))
140 +|(((
141 ++y
142 +)))|(((
143 +up
144 +)))|(((
145 +1.0 - 0.5 mm
146 +)))|(% colspan="1" %)(((
147 +0.1 - 0.2 mm
148 +)))
149 +|(((
150 ++z
151 +)))|(((
152 +towards detector tower, away from tape
153 +)))|(((
154 +0.5 - 0.2 mm
155 +)))|(% colspan="1" %)(((
156 +0.1 - 0.2 mm
157 +be careful with -z, tape could be pushed out of focus
158 +)))
92 92  
160 +**
161 +\\**
162 +)))
163 +* [[image:attach:kamzik_nozzle.PNG||height="400"]]
164 +
165 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
166 +
93 93  \\
94 94  
169 +\\
170 +
171 +\\
172 +
173 +\\
174 +
95 95  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
96 96  
97 97   xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
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102 102  
103 103  \\
104 104  
105 -**14.5 Tape roll exchange**
185 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ====
106 106  
107 -**[[image:attach:20211201_094111.jpg||height="250"]][[image:attach:20211201_094101.jpg||height="250"]]**
187 +The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
108 108  
189 +* Remove the (almost) empty feeder roll by pulling it upwards.
190 +* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
191 +* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
192 +* Cut the first cm of the new tape roll, directly before the black sticky part.
193 +* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
194 +* Open magnetic tape stabilizer.
195 +* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
196 +* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
197 +* Attach new tape to the empty roll by using the black sticky part.
198 +* Close tape stabilizer.
199 +
200 +**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
201 +**
202 +
109 109  \\
110 110  
111 111  \\
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235037709
1 +235039466
URL
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1 -https://confluence.desy.de/spaces/11/pages/235037709/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235039466/14. Serial Crystallography: CFEL TapeDrive