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Last modified by chatzies on 2025-11-27 11:35
From version 21.1
edited by ahenkel
on 2021-12-03 11:18
Change comment: There is no comment for this version
To version 18.1
edited by ahenkel
on 2021-12-02 14:20
Change comment: There is no comment for this version

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65 65  **14.3 Connecting sample to ElveFlow, sample exchange
66 66  **
67 67  
68 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bttom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
68 +[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]][[image:attach:20211201_095205.jpg||height="250"]][[image:attach:20211201_095232.jpg||height="250"]]
69 69  
70 -Be aware to which channel and nozzle line your sample is connected.
71 -
72 -Red → Channel 1, crystal line (nozzle capillary without marks)
73 -
74 -Green → Channel 2, substrate line (nozzle capillary is marked in black)
75 -
76 -[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
77 -
78 78  \\
79 79  
80 80  \\
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82 82  **14.4 TapeDrive operation
83 83  **
84 84  
85 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
77 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open, shortly after a small window.
86 86  Now start the client. Same thing, a terminal will open, shortly after a small window.
87 87  
88 88  Click 'Devices' in the **client session** to open the control window.
... ... @@ -93,7 +93,6 @@
93 93  ** Change the velocity by typing anything from **0.3-3.0 mm/s**
94 94  ** Common velocity is 1.0-1.5 mm/s
95 95  ** kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
96 -\\
97 97  ** [[image:attach:kamzik_tape.PNG||height="400"]]
98 98  
99 99  \\
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101 101  * **Sample nozzle:**
102 102  ** Click an the 'nozzle' icon.
103 103  ** (((
104 -The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
95 +The nozzle position can be adjusted in xyz by clicking on the arrows up and down. Step size can be varied, 1mm steps are rather big and should be used carefully, for y-movement always use a stepsize <= 0.5mm.
105 105  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
106 -\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
107 -\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
97 +\\Adjust the nozzle position visually until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the X-ray beam (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.
108 108  
109 109  (% class="wrapped" %)
110 110  |=(((
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147 147  be careful with -z, tape could be pushed out of focus
148 148  )))
149 149  
150 -**
140 +(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)**
151 151  \\**
152 152  )))
153 153  ** [[image:attach:kamzik_nozzle.PNG||height="400"]]
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170 170  
171 171  **14.5 Tape roll exchange**
172 172  
173 -The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
163 +**[[image:attach:20211201_094111.jpg||height="250"]][[image:attach:20211201_094101.jpg||height="250"]]**
174 174  
175 -* Remove the (almost) empty feeder roll by pulling it upwards.
176 -* Cut the tape with some cm overlap to glue to the new roll.
177 -* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
178 -* Cut the first cm of the new tape roll, directly before the black sticky part.
179 -* Use this sticky part to attach the end of the old
180 -
181 -**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
182 -**
183 -
184 184  \\
185 185  
186 186  \\
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235039450
1 +235039093
URL
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1 -https://confluence.desy.de/spaces/11/pages/235039450/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235039093/14. Serial Crystallography: CFEL TapeDrive