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Last modified by chatzies on 2025-11-27 11:35
From version 21.1
edited by ahenkel
on 2021-12-03 11:18
Change comment: There is no comment for this version
To version 22.1
edited by ahenkel
on 2021-12-03 11:26
Change comment: There is no comment for this version

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... ... @@ -65,13 +65,13 @@
65 65  **14.3 Connecting sample to ElveFlow, sample exchange
66 66  **
67 67  
68 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bttom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
68 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
69 69  
70 70  Be aware to which channel and nozzle line your sample is connected.
71 71  
72 -Red → Channel 1, crystal line (nozzle capillary without marks)
72 + Red → Channel 1, crystal line (nozzle capillary without marks)
73 73  
74 -Green → Channel 2, substrate line (nozzle capillary is marked in black)
74 + Green → Channel 2, substrate line (nozzle capillary is marked in black)
75 75  
76 76  [[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
77 77  
... ... @@ -173,10 +173,15 @@
173 173  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
174 174  
175 175  * Remove the (almost) empty feeder roll by pulling it upwards.
176 -* Cut the tape with some cm overlap to glue to the new roll.
176 +* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
177 177  * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
178 178  * Cut the first cm of the new tape roll, directly before the black sticky part.
179 -* Use this sticky part to attach the end of the old
179 +* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
180 +* Open magnetic tape stabilizer.
181 +* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
182 +* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
183 +* Attach new tape to the empty roll by using the black sticky part.
184 +* Close tape stabilizer.
180 180  
181 181  **[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
182 182  **
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235039450
1 +235039461
URL
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1 -https://confluence.desy.de/spaces/11/pages/235039450/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235039461/14. Serial Crystallography: CFEL TapeDrive