Changes for page CFEL TapeDrive: Serial Crystallography

Last modified by chatzies on 2025-11-27 11:35

From 20.1 to 21.1 From 41.1 to 42.1

From version 21.1

edited by ahenkel
on 2021-12-03 11:18

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To version 41.1

edited by ahenkel
on 2021-12-12 23:20

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@@ -1,7 +1,20 @@
--[[image:attach:TapeDrive_beschriftet.PNG||height="400"]][[image:attach:TapeDrive scheme.png||height="400"]]
++{{layout}}
++{{layout-section ac:type="single"}}
++{{layout-cell}}
--**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
++{{toc/}}
++
++[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
++
++\\
++
++\\
++
++\\
++
++=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
++
  * remove vortex
  * beamstop to out-position?
  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
@@ -50,7 +50,7 @@
  \\
--**14.2 Sample characteristics and preparation**
++=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
@@ -62,25 +62,27 @@
  \\
--**14.3 Connecting sample to ElveFlow, sample exchange
--**
++=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
++** ===
--After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bttom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
++After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
  Be aware to which channel and nozzle line your sample is connected.
--Red → Channel 1, crystal line (nozzle capillary without marks)
++            Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
--Green → Channel 2, substrate line (nozzle capillary is marked in black)
++            Green → Channel 2, substrate line (nozzle capillary is marked in black)
  [[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
--\\
++After finishing data collection, flush all lines by connecting an Eppi or syringe filled with ddH,,2,,O.
++[[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]]
++
  \\
--**14.4 TapeDrive operation
--**
++=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
++** ===
  The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
  Now start the client. Same thing, a terminal will open, shortly after a small window.
@@ -88,24 +88,26 @@
  Click 'Devices' in the **client session** to open the control window.
  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
--* **Tape:**
--** Click on the 'main tape' icon
--** Change the velocity by typing anything from **0.3-3.0 mm/s**
--** Common velocity is 1.0-1.5 mm/s
--** kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
--\\
--** [[image:attach:kamzik_tape.PNG||height="400"]]
++**Tape**
++* Click on the 'main tape' icon
++* Change the velocity by typing anything from **0.3-3.0 mm/s**
++* Common velocity is 1.0-1.5 mm/s
++* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
  \\
++* [[image:attach:kamzik_tape.PNG||height="400"]]
--* **Sample nozzle:**
--** Click an the 'nozzle' icon.
--** (((
++==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
++
++* Click an the 'nozzle' icon.
++* (((
  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
--\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
--\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
++
++(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
++\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
++
  (% class="wrapped" %)
  |=(((
  \\
@@ -150,12 +150,20 @@
  **
  \\**
  )))
--** [[image:attach:kamzik_nozzle.PNG||height="400"]]
++* [[image:attach:kamzik_nozzle.PNG||height="400"]]
++==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
++
++* The chiller circulates fluid through the gas nozzle.
++
  \\
++==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ====
++
  \\
++==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ====
++
  \\
  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
@@ -168,17 +168,23 @@
  \\
--**14.5 Tape roll exchange**
++=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ===
  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
  * Remove the (almost) empty feeder roll by pulling it upwards.
--* Cut the tape with some cm overlap to glue to the new roll.
++* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
  * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
--* Cut the first cm of the new tape roll, directly before the black sticky part.
--* Use this sticky part to attach the end of the old
++* Cut the first cm of the new tape roll, directly behind the black sticky part.
++* Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll.
++* Open magnetic tape stabilizer on the **left side**. //!!!BEAMSTOP!!!//
++* Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s).
++* Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded.
++* Attach new tape to the empty roll by using some sticky tape.
++* Close tape stabilizer.
++* Reset tape counter in kamzik by clicking "<Call>" in "Main-Tape".
--**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
++**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]
  **
  \\
@@ -185,8 +185,170 @@
  \\
++=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}**14.6 Nozzle exchange** ===
++
  \\
++=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Datacollection"/}}**14.7 Data collection** ===
++
++Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
++
++(% class="code" %)
++(((
++                      xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
++                      password: written on control laptop (changes every 6 months)
++)))
++
++                                           //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
++
  \\
++Click "Start" icon in kamzik.
++
++[[image:attach:kamzik_run-tile.jpg||height="400"]]
++
++----
++{{/layout-cell}}
++{{/layout-section}}
++
++{{layout-section ac:type="two_equal"}}
++{{layout-cell}}
++**Check list:**
++
++(% style="color: rgb(255,0,0);" %)//always after entering the hutch!//
++
++* Open BS1  (//CrystalControl)//
++* Put collimator in (//CrystalControl)//
++* Check beamstop position = 27 000 (//Atk panel p11/motor/eh.3.09)//
++* Open fast shutter (//CrystalControl)//
++* Check diode voltage ~~ 0.0 (//kamzik Run/Diode voltage)//
++* Close fast shutter (//CrystalControl)//
++
  \\
++
++**Set run parameters and go 🏃💥**
++
++1. Set your desired No. of frames
++1. Match exposure time with chopper setting
++1. Check trigger setting and match to chopper setting
++1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
++1. To start the run, click "<Call>" next to //exposure time//
++
++\\
++
++(% style="color: rgb(153,51,102);" %)**//Current bug~://**
++
++(% style="color: rgb(0,0,0);" %)You need to "Stop" run in kamzik manually after all images have been acquired. This moves the shield up and closes the fast shutter. Afterwards you can start a new run by clicking "<Call>".
++
++
++(% style="color: rgb(0,0,0);" %)Check progress in terminal with:
++
++(% class="code" %)
++(((
++      cd /gpfs/current/raw/scan_frames
++      watch "ls -lrt <run number>/ | tail"
++\\\\\\\\
++)))
++{{/layout-cell}}
++
++{{layout-cell}}
++(% class="wrapped relative-table" style="width: 100.0%;" %)
++|=(((
++Parameter
++)))|=(((
++explanation
++)))|=(% colspan="1" %)(((
++value
++)))
++|(((
++Images number
++)))|(((
++Set number of frames you want to collect
++)))|(% colspan="1" %)(((
++usually 100k - 200k, but adjust according to hitrate
++
++orientation: 10 % hitrate needs at least 100k images
++)))
++|(((
++exposure
++)))|(((
++exposure time of Eiger
++
++\\
++)))|(% colspan="1" %)(((
++chopper at 133 Hz → 3.7 ms
++
++chopper at 66 Hz   → 7.7 ms
++
++no chopper            → 7.7 ms
++
++----
++)))
++|(((
++run id
++)))|(((
++changes automatically
++)))|(% colspan="1" %)(((
++\\
++)))
++|(% colspan="1" %)(((
++Detector distance
++)))|(% colspan="1" %)(((
++current detector postion readout
++)))|(% colspan="1" %)(((
++\\
++)))
++|(% colspan="1" %)(((
++move_detector (distance)
++)))|(% colspan="1" %)(((
++Set detector distance
++)))|(% colspan="1" %)(((
++200.0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
++)))
++|(% colspan="1" %)(((
++Diode voltage
++)))|(% colspan="1" %)(((
++read out of diode
++)))|(% colspan="1" %)(((
++**~~ 0   !!! higher values indicate missaligned beam stop!!!
++Call local contact
++**
++)))
++{{/layout-cell}}
++{{/layout-section}}
++
++{{layout-section ac:type="single"}}
++{{layout-cell}}
++----
++
++=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Troubleshooting"/}}14.8 Troubleshooting ===
++
++* (((
++==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
++
++* Tape roll empty?
++* motor connected (icon green)?
++)))
++
++\\
++
++* (((
++==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
++
++* displayed in green=connected?
++* nozzle holder colliding somewhere?
++* motor limits reached? → position displayed in red
++)))
++
++\\
++
++* (((
++==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
++
++* channel turned on?
++* Sample empty?
++* Nozzle or lines clogged? → flush with water, change nozzle
++)))
++{{/layout-cell}}
++{{/layout-section}}
++{{/layout}}

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@@ -1,1 +1,1 @@
--235039450
++236824588

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@@ -1,1 +1,1 @@
--https://confluence.desy.de/spaces/11/pages/235039450/14. Serial Crystallography: CFEL TapeDrive
++https://confluence.desy.de/spaces/11/pages/236824588/14. Serial Crystallography: CFEL TapeDrive

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