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Last modified by chatzies on 2025-11-27 11:35
From version 22.1
edited by ahenkel
on 2021-12-03 11:26
Change comment: There is no comment for this version
To version 11.1
edited by ahenkel
on 2021-12-01 11:31
Change comment: There is no comment for this version

Summary

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... ... @@ -58,99 +58,20 @@
58 58  
59 59  * filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
60 60  
61 -[[image:attach:20211201_095534.jpg||height="250"]]
62 -
63 63  \\
64 64  
65 -**14.3 Connecting sample to ElveFlow, sample exchange
66 -**
67 -
68 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
69 -
70 -Be aware to which channel and nozzle line your sample is connected.
71 -
72 - Red → Channel 1, crystal line (nozzle capillary without marks)
73 -
74 - Green → Channel 2, substrate line (nozzle capillary is marked in black)
75 -
76 -[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
77 -
78 78  \\
79 79  
80 -\\
65 +**14.3 Connecting sample to ElveFlow**
81 81  
82 -**14.4 TapeDrive operation
83 -**
84 -
85 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
86 -Now start the client. Same thing, a terminal will open, shortly after a small window.
87 -
88 -Click 'Devices' in the **client session** to open the control window.
89 -[[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
90 -
91 -* **Tape:**
92 -** Click on the 'main tape' icon
93 -** Change the velocity by typing anything from **0.3-3.0 mm/s**
94 -** Common velocity is 1.0-1.5 mm/s
95 -** kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
96 96  \\
97 -** [[image:attach:kamzik_tape.PNG||height="400"]]
98 98  
99 99  \\
100 100  
101 -* **Sample nozzle:**
102 -** Click an the 'nozzle' icon.
103 -** (((
104 -The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
105 -The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
106 -\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
107 -\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
108 -
109 -(% class="wrapped" %)
110 -|=(((
111 111  \\
112 -)))|=(((
113 -direction
114 -)))|=(((
115 -recommended step size
116 -for visual adjustment
117 -)))|=(% colspan="1" %)(((
118 -recommended step size
119 -during data collection
120 -)))
121 -|(((
122 -+x
123 -)))|(((
124 -away from X-ray interaction point
125 -)))|(((
126 -1.0 - 0.5 mm
127 -)))|(% colspan="1" %)(((
128 -0.1 - 0.2 mm
129 -)))
130 -|(((
131 -+y
132 -)))|(((
133 -up
134 -)))|(((
135 -1.0 - 0.5 mm
136 -)))|(% colspan="1" %)(((
137 -0.1 - 0.2 mm
138 -)))
139 -|(((
140 -+z
141 -)))|(((
142 -towards detector tower, away from tape
143 -)))|(((
144 -0.5 - 0.2 mm
145 -)))|(% colspan="1" %)(((
146 -0.1 - 0.2 mm
147 -be careful with -z, tape could be pushed out of focus
148 -)))
149 149  
73 +**14.4 TapeDrive operation
150 150  **
151 -\\**
152 -)))
153 -** [[image:attach:kamzik_nozzle.PNG||height="400"]]
154 154  
155 155  \\
156 156  
... ... @@ -158,40 +158,6 @@
158 158  
159 159  \\
160 160  
161 -Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
162 -
163 - xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
164 - password: //written on control laptop (changes every 6 months)
165 -//
166 -
167 167  \\
168 168  
169 169  \\
170 -
171 -**14.5 Tape roll exchange**
172 -
173 -The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
174 -
175 -* Remove the (almost) empty feeder roll by pulling it upwards.
176 -* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
177 -* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
178 -* Cut the first cm of the new tape roll, directly before the black sticky part.
179 -* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
180 -* Open magnetic tape stabilizer.
181 -* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
182 -* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
183 -* Attach new tape to the empty roll by using the black sticky part.
184 -* Close tape stabilizer.
185 -
186 -**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
187 -**
188 -
189 -\\
190 -
191 -\\
192 -
193 -\\
194 -
195 -\\
196 -
197 -\\
Confluence.Code.ConfluencePageClass[0]
Id
... ... @@ -1,1 +1,1 @@
1 -235039461
1 +235034364
URL
... ... @@ -1,1 +1,1 @@
1 -https://confluence.desy.de/spaces/11/pages/235039461/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235034364/14. Serial Crystallography: CFEL TapeDrive