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Last modified by chatzies on 2025-11-27 11:35
From version 23.1
edited by ahenkel
on 2021-12-03 11:32
Change comment: There is no comment for this version
To version 21.1
edited by ahenkel
on 2021-12-03 11:18
Change comment: There is no comment for this version

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1 1  [[image:attach:TapeDrive_beschriftet.PNG||height="400"]][[image:attach:TapeDrive scheme.png||height="400"]]
2 2  
3 -\\
3 +**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
4 4  
5 -\\
6 -
7 -\\
8 -
9 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
10 -
11 11  * remove vortex
12 12  * beamstop to out-position?
13 13  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
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56 56  
57 57  \\
58 58  
59 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
53 +**14.2 Sample characteristics and preparation**
60 60  
61 61  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
62 62  
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68 68  
69 69  \\
70 70  
71 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
72 -** ===
65 +**14.3 Connecting sample to ElveFlow, sample exchange
66 +**
73 73  
74 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
68 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bttom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
75 75  
76 76  Be aware to which channel and nozzle line your sample is connected.
77 77  
78 - Red → Channel 1, crystal line (nozzle capillary without marks)
72 +Red → Channel 1, crystal line (nozzle capillary without marks)
79 79  
80 - Green → Channel 2, substrate line (nozzle capillary is marked in black)
74 +Green → Channel 2, substrate line (nozzle capillary is marked in black)
81 81  
82 82  [[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
83 83  
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85 85  
86 86  \\
87 87  
88 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
89 -** ===
82 +**14.4 TapeDrive operation
83 +**
90 90  
91 91  The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
92 92  Now start the client. Same thing, a terminal will open, shortly after a small window.
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94 94  Click 'Devices' in the **client session** to open the control window.
95 95  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
96 96  
97 -**Tape**
91 +* **Tape:**
92 +** Click on the 'main tape' icon
93 +** Change the velocity by typing anything from **0.3-3.0 mm/s**
94 +** Common velocity is 1.0-1.5 mm/s
95 +** kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
96 +\\
97 +** [[image:attach:kamzik_tape.PNG||height="400"]]
98 98  
99 -* Click on the 'main tape' icon
100 -* Change the velocity by typing anything from **0.3-3.0 mm/s**
101 -* Common velocity is 1.0-1.5 mm/s
102 -* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
103 103  \\
104 -* [[image:attach:kamzik_tape.PNG||height="400"]]
105 105  
106 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle:"/}}**Sample nozzle:** ====
107 -
108 -* Click an the 'nozzle' icon.
109 -* (((
101 +* **Sample nozzle:**
102 +** Click an the 'nozzle' icon.
103 +** (((
110 110  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
111 111  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
112 112  \\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
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156 156  **
157 157  \\**
158 158  )))
159 -* [[image:attach:kamzik_nozzle.PNG||height="400"]]
153 +** [[image:attach:kamzik_nozzle.PNG||height="400"]]
160 160  
161 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
162 -
163 163  \\
164 164  
165 165  \\
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166 166  
167 167  \\
168 168  
169 -\\
170 -
171 171  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
172 172  
173 173   xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
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178 178  
179 179  \\
180 180  
181 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ====
171 +**14.5 Tape roll exchange**
182 182  
183 183  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
184 184  
185 185  * Remove the (almost) empty feeder roll by pulling it upwards.
186 -* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
176 +* Cut the tape with some cm overlap to glue to the new roll.
187 187  * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
188 188  * Cut the first cm of the new tape roll, directly before the black sticky part.
189 -* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
190 -* Open magnetic tape stabilizer.
191 -* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
192 -* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
193 -* Attach new tape to the empty roll by using the black sticky part.
194 -* Close tape stabilizer.
179 +* Use this sticky part to attach the end of the old
195 195  
196 196  **[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
197 197  **
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235039462
1 +235039450
URL
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1 -https://confluence.desy.de/spaces/11/pages/235039462/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235039450/14. Serial Crystallography: CFEL TapeDrive