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Last modified by chatzies on 2025-11-27 11:35
From version 25.1
edited by ahenkel
on 2021-12-03 11:34
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To version 17.1
edited by ahenkel
on 2021-12-02 14:15
Change comment: There is no comment for this version

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1 +[[image:attach:TapeDrive_beschriftet.PNG||height="400"]][[image:attach:TapeDrive scheme.png||height="400"]]
1 1  
3 +**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
2 2  
3 -{{toc/}}
4 -
5 -[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
6 -
7 -\\
8 -
9 -\\
10 -
11 -\\
12 -
13 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
14 -
15 15  * remove vortex
16 16  * beamstop to out-position?
17 17  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
... ... @@ -60,7 +60,7 @@
60 60  
61 61  \\
62 62  
63 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
53 +**14.2 Sample characteristics and preparation**
64 64  
65 65  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
66 66  
... ... @@ -72,51 +72,42 @@
72 72  
73 73  \\
74 74  
75 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
76 -** ===
65 +**14.3 Connecting sample to ElveFlow, sample exchange
66 +**
77 77  
78 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
68 +[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]][[image:attach:20211201_095205.jpg||height="250"]][[image:attach:20211201_095232.jpg||height="250"]]
79 79  
80 -Be aware to which channel and nozzle line your sample is connected.
81 -
82 - Red → Channel 1, crystal line (nozzle capillary without marks)
83 -
84 - Green → Channel 2, substrate line (nozzle capillary is marked in black)
85 -
86 -[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
87 -
88 88  \\
89 89  
90 90  \\
91 91  
92 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
93 -** ===
74 +**14.4 TapeDrive operation
75 +**
94 94  
95 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
77 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open, shortly after a small window.
96 96  Now start the client. Same thing, a terminal will open, shortly after a small window.
97 97  
98 98  Click 'Devices' in the **client session** to open the control window.
99 99  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
100 100  
101 -**Tape**
83 +* **Tape:**
84 +** Click on the 'main tape' icon
85 +** Change the velocity by typing anything from 0.3-3.0 mm/s
86 +** Common velocity is 1.0-1.5 mm/s
87 +** kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
88 +** [[image:attach:kamzik_tape.PNG||height="400"]]
102 102  
103 -* Click on the 'main tape' icon
104 -* Change the velocity by typing anything from **0.3-3.0 mm/s**
105 -* Common velocity is 1.0-1.5 mm/s
106 -* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
107 107  \\
108 -* [[image:attach:kamzik_tape.PNG||height="400"]]
109 109  
110 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle:"/}}**Sample nozzle:** ====
111 -
112 -* Click an the 'nozzle' icon.
113 -* (((
114 -The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
92 +* **Sample nozzle:**
93 +** Click an the 'nozzle' icon.
94 +** (((
95 +The nozzle position can be adjusted in xyz by clicking on the arrows up and down. Step size can be varied, 1mm steps are rather big and should be used carefully, for y-movement always use a stepsize <= 0.5mm.
115 115  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
116 -\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
117 -\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
97 ++x    right, away from X-ray interaction point
98 ++y    up
99 ++z    towards detector tower, away from the tape
118 118  
119 -(% class="wrapped" %)
120 120  |=(((
121 121  \\
122 122  )))|=(((
... ... @@ -131,7 +131,7 @@
131 131  |(((
132 132  +x
133 133  )))|(((
134 -away from X-ray interaction point
115 +right, away from X-ray interaction point
135 135  )))|(((
136 136  1.0 - 0.5 mm
137 137  )))|(% colspan="1" %)(((
... ... @@ -157,13 +157,11 @@
157 157  be careful with -z, tape could be pushed out of focus
158 158  )))
159 159  
160 -**
141 +(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)**
161 161  \\**
162 162  )))
163 -* [[image:attach:kamzik_nozzle.PNG||height="400"]]
144 +** [[image:attach:kamzik_nozzle.PNG||height="400"]]
164 164  
165 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
166 -
167 167  \\
168 168  
169 169  \\
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170 170  
171 171  \\
172 172  
173 -\\
174 -
175 175  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
176 176  
177 177   xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
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182 182  
183 183  \\
184 184  
185 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ====
162 +**14.5 Tape roll exchange**
186 186  
187 -The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
164 +**[[image:attach:20211201_094111.jpg||height="250"]][[image:attach:20211201_094101.jpg||height="250"]]**
188 188  
189 -* Remove the (almost) empty feeder roll by pulling it upwards.
190 -* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
191 -* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
192 -* Cut the first cm of the new tape roll, directly before the black sticky part.
193 -* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
194 -* Open magnetic tape stabilizer.
195 -* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
196 -* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
197 -* Attach new tape to the empty roll by using the black sticky part.
198 -* Close tape stabilizer.
199 -
200 -**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
201 -**
202 -
203 203  \\
204 204  
205 205  \\
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235039466
1 +235037729
URL
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1 -https://confluence.desy.de/spaces/11/pages/235039466/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235037729/14. Serial Crystallography: CFEL TapeDrive