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Last modified by chatzies on 2025-11-27 11:35
From version 26.1
edited by ahenkel
on 2021-12-03 11:35
Change comment: There is no comment for this version
To version 10.1
edited by ahenkel
on 2021-12-01 11:25
Change comment: There is no comment for this version

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1 +[[image:attach:TapeDrive_beschriftet.PNG||height="250"]][[image:attach:TapeDrive scheme.png||height="250"]]
1 1  
3 +**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
2 2  
3 -{{toc/}}
4 -
5 -[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
6 -
7 -\\
8 -
9 -\\
10 -
11 -\\
12 -
13 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
14 -
15 15  * remove vortex
16 16  * beamstop to out-position?
17 17  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
... ... @@ -60,7 +60,7 @@
60 60  
61 61  \\
62 62  
63 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
53 +**14.2 Sample characteristics and preparation**
64 64  
65 65  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
66 66  
... ... @@ -68,136 +68,19 @@
68 68  
69 69  * filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
70 70  
71 -[[image:attach:20211201_095534.jpg||height="250"]]
72 -
73 73  \\
74 74  
75 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
76 -** ===
77 -
78 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
79 -
80 -Be aware to which channel and nozzle line your sample is connected.
81 -
82 - Red → Channel 1, crystal line (nozzle capillary without marks)
83 -
84 - Green → Channel 2, substrate line (nozzle capillary is marked in black)
85 -
86 -[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
87 -
88 88  \\
89 89  
90 -\\
65 +**14.3 Connecting sample to ElveFlow**
91 91  
92 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
93 -** ===
94 -
95 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
96 -Now start the client. Same thing, a terminal will open, shortly after a small window.
97 -
98 -Click 'Devices' in the **client session** to open the control window.
99 -[[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
100 -
101 -**Tape**
102 -
103 -* Click on the 'main tape' icon
104 -* Change the velocity by typing anything from **0.3-3.0 mm/s**
105 -* Common velocity is 1.0-1.5 mm/s
106 -* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
107 107  \\
108 -* [[image:attach:kamzik_tape.PNG||height="400"]]
109 109  
110 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
111 -
112 -* Click an the 'nozzle' icon.
113 -* (((
114 -The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
115 -The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
116 -\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
117 -\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
118 -
119 -(% class="wrapped" %)
120 -|=(((
121 121  \\
122 -)))|=(((
123 -direction
124 -)))|=(((
125 -recommended step size
126 -for visual adjustment
127 -)))|=(% colspan="1" %)(((
128 -recommended step size
129 -during data collection
130 -)))
131 -|(((
132 -+x
133 -)))|(((
134 -away from X-ray interaction point
135 -)))|(((
136 -1.0 - 0.5 mm
137 -)))|(% colspan="1" %)(((
138 -0.1 - 0.2 mm
139 -)))
140 -|(((
141 -+y
142 -)))|(((
143 -up
144 -)))|(((
145 -1.0 - 0.5 mm
146 -)))|(% colspan="1" %)(((
147 -0.1 - 0.2 mm
148 -)))
149 -|(((
150 -+z
151 -)))|(((
152 -towards detector tower, away from tape
153 -)))|(((
154 -0.5 - 0.2 mm
155 -)))|(% colspan="1" %)(((
156 -0.1 - 0.2 mm
157 -be careful with -z, tape could be pushed out of focus
158 -)))
159 159  
160 -**
161 -\\**
162 -)))
163 -* [[image:attach:kamzik_nozzle.PNG||height="400"]]
164 -
165 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
166 -
167 167  \\
168 168  
169 -\\
170 -
171 -\\
172 -
173 -\\
174 -
175 -Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
176 -
177 - xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
178 - password: //written on control laptop (changes every 6 months)
179 -//
180 -
181 -\\
182 -
183 -\\
184 -
185 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ====
186 -
187 -The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
188 -
189 -* Remove the (almost) empty feeder roll by pulling it upwards.
190 -* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
191 -* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
192 -* Cut the first cm of the new tape roll, directly before the black sticky part.
193 -* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
194 -* Open magnetic tape stabilizer.
195 -* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
196 -* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
197 -* Attach new tape to the empty roll by using the black sticky part.
198 -* Close tape stabilizer.
199 -
200 -**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
73 +**14.4 TapeDrive operation
201 201  **
202 202  
203 203  \\
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235041512
1 +235034346
URL
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1 -https://confluence.desy.de/spaces/11/pages/235041512/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235034346/14. Serial Crystallography: CFEL TapeDrive