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Last modified by chatzies on 2025-11-27 11:35
From version 28.1
edited by ahenkel
on 2021-12-05 20:05
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To version 20.1
edited by ahenkel
on 2021-12-03 11:10
Change comment: There is no comment for this version

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1 +[[image:attach:TapeDrive_beschriftet.PNG||height="400"]][[image:attach:TapeDrive scheme.png||height="400"]]
1 1  
3 +**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
2 2  
3 -{{toc/}}
4 -
5 -[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
6 -
7 -\\
8 -
9 -\\
10 -
11 -\\
12 -
13 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
14 -
15 15  * remove vortex
16 16  * beamstop to out-position?
17 17  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
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60 60  
61 61  \\
62 62  
63 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
53 +**14.2 Sample characteristics and preparation**
64 64  
65 65  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
66 66  
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72 72  
73 73  \\
74 74  
75 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
76 -** ===
65 +**14.3 Connecting sample to ElveFlow, sample exchange
66 +**
77 77  
78 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
68 +[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]][[image:attach:20211201_095205.jpg||height="250"]][[image:attach:20211201_095232.jpg||height="250"]]
79 79  
80 -Be aware to which channel and nozzle line your sample is connected.
70 +\\
81 81  
82 - Red → Channel 1, crystal line (nozzle capillary without marks)
83 -
84 - Green → Channel 2, substrate line (nozzle capillary is marked in black)
85 -
86 -[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
87 -
88 -After finishing data collection, flush all lines by connecting an Eppi or syringe filled with ddH,,2,,O.
89 -
90 -[[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]]
91 -
92 92  \\
93 93  
94 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
95 -** ===
74 +**14.4 TapeDrive operation
75 +**
96 96  
97 97  The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
98 98  Now start the client. Same thing, a terminal will open, shortly after a small window.
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100 100  Click 'Devices' in the **client session** to open the control window.
101 101  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
102 102  
103 -**Tape**
83 +* **Tape:**
84 +** Click on the 'main tape' icon
85 +** Change the velocity by typing anything from **0.3-3.0 mm/s**
86 +** Common velocity is 1.0-1.5 mm/s
87 +** kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
88 +\\
89 +** [[image:attach:kamzik_tape.PNG||height="400"]]
104 104  
105 -* Click on the 'main tape' icon
106 -* Change the velocity by typing anything from **0.3-3.0 mm/s**
107 -* Common velocity is 1.0-1.5 mm/s
108 -* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
109 109  \\
110 -* [[image:attach:kamzik_tape.PNG||height="400"]]
111 111  
112 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
113 -
114 -* Click an the 'nozzle' icon.
115 -* (((
93 +* **Sample nozzle:**
94 +** Click an the 'nozzle' icon.
95 +** (((
116 116  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
117 117  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
118 118  \\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
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162 162  **
163 163  \\**
164 164  )))
165 -* [[image:attach:kamzik_nozzle.PNG||height="400"]]
145 +** [[image:attach:kamzik_nozzle.PNG||height="400"]]
166 166  
167 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
168 -
169 169  \\
170 170  
171 171  \\
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172 172  
173 173  \\
174 174  
175 -\\
176 -
177 177  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
178 178  
179 179   xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
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184 184  
185 185  \\
186 186  
187 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ===
163 +**14.5 Tape roll exchange**
188 188  
189 189  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
190 190  
191 191  * Remove the (almost) empty feeder roll by pulling it upwards.
192 -* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
168 +* Cut the tape with some cm overlap to glue to the new roll.
193 193  * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
194 194  * Cut the first cm of the new tape roll, directly before the black sticky part.
195 -* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
196 -* Open magnetic tape stabilizer.
197 -* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
198 -* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
199 -* Attach new tape to the empty roll by using the black sticky part.
200 -* Close tape stabilizer.
171 +* Use this sticky part to attach the end of the old
201 201  
202 202  **[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
203 203  **
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206 206  
207 207  \\
208 208  
209 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}14.6 Nozzle exchange ===
210 -
211 211  \\
212 212  
213 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Datacollection"/}}14.7 Data collection ===
214 -
215 215  \\
216 216  
217 217  \\
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235041525
1 +235039424
URL
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1 -https://confluence.desy.de/spaces/11/pages/235041525/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235039424/14. Serial Crystallography: CFEL TapeDrive