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Last modified by chatzies on 2025-11-27 11:35
From version 30.1
edited by ahenkel
on 2021-12-07 17:44
Change comment: There is no comment for this version
To version 18.1
edited by ahenkel
on 2021-12-02 14:20
Change comment: There is no comment for this version

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1 +[[image:attach:TapeDrive_beschriftet.PNG||height="400"]][[image:attach:TapeDrive scheme.png||height="400"]]
1 1  
3 +**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
2 2  
3 -{{toc/}}
4 -
5 -[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
6 -
7 -\\
8 -
9 -\\
10 -
11 -\\
12 -
13 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
14 -
15 15  * remove vortex
16 16  * beamstop to out-position?
17 17  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
... ... @@ -60,7 +60,7 @@
60 60  
61 61  \\
62 62  
63 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
53 +**14.2 Sample characteristics and preparation**
64 64  
65 65  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
66 66  
... ... @@ -72,51 +72,39 @@
72 72  
73 73  \\
74 74  
75 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
76 -** ===
65 +**14.3 Connecting sample to ElveFlow, sample exchange
66 +**
77 77  
78 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
68 +[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]][[image:attach:20211201_095205.jpg||height="250"]][[image:attach:20211201_095232.jpg||height="250"]]
79 79  
80 -Be aware to which channel and nozzle line your sample is connected.
70 +\\
81 81  
82 - Red → Channel 1, crystal line (nozzle capillary without marks)
83 -
84 - Green → Channel 2, substrate line (nozzle capillary is marked in black)
85 -
86 -[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
87 -
88 -After finishing data collection, flush all lines by connecting an Eppi or syringe filled with ddH,,2,,O.
89 -
90 -[[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]]
91 -
92 92  \\
93 93  
94 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
95 -** ===
74 +**14.4 TapeDrive operation
75 +**
96 96  
97 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
77 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open, shortly after a small window.
98 98  Now start the client. Same thing, a terminal will open, shortly after a small window.
99 99  
100 100  Click 'Devices' in the **client session** to open the control window.
101 101  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
102 102  
103 -**Tape**
83 +* **Tape:**
84 +** Click on the 'main tape' icon
85 +** Change the velocity by typing anything from **0.3-3.0 mm/s**
86 +** Common velocity is 1.0-1.5 mm/s
87 +** kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
88 +** [[image:attach:kamzik_tape.PNG||height="400"]]
104 104  
105 -* Click on the 'main tape' icon
106 -* Change the velocity by typing anything from **0.3-3.0 mm/s**
107 -* Common velocity is 1.0-1.5 mm/s
108 -* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
109 109  \\
110 -* [[image:attach:kamzik_tape.PNG||height="400"]]
111 111  
112 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
113 -
114 -* Click an the 'nozzle' icon.
115 -* (((
116 -The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
92 +* **Sample nozzle:**
93 +** Click an the 'nozzle' icon.
94 +** (((
95 +The nozzle position can be adjusted in xyz by clicking on the arrows up and down. Step size can be varied, 1mm steps are rather big and should be used carefully, for y-movement always use a stepsize <= 0.5mm.
117 117  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
118 -\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
119 -\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
97 +\\Adjust the nozzle position visually until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the X-ray beam (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.
120 120  
121 121  (% class="wrapped" %)
122 122  |=(((
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159 159  be careful with -z, tape could be pushed out of focus
160 160  )))
161 161  
162 -**
140 +(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)**
163 163  \\**
164 164  )))
165 -* [[image:attach:kamzik_nozzle.PNG||height="400"]]
143 +** [[image:attach:kamzik_nozzle.PNG||height="400"]]
166 166  
167 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
168 -
169 -* The chiller circulates fluid through the gas\\
170 -
171 171  \\
172 172  
173 173  \\
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174 174  
175 175  \\
176 176  
177 -\\
178 -
179 179  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
180 180  
181 181   xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
... ... @@ -186,48 +186,16 @@
186 186  
187 187  \\
188 188  
189 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ===
161 +**14.5 Tape roll exchange**
190 190  
191 -The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
163 +**[[image:attach:20211201_094111.jpg||height="250"]][[image:attach:20211201_094101.jpg||height="250"]]**
192 192  
193 -* Remove the (almost) empty feeder roll by pulling it upwards.
194 -* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
195 -* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
196 -* Cut the first cm of the new tape roll, directly before the black sticky part.
197 -* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
198 -* Open magnetic tape stabilizer.
199 -* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
200 -* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
201 -* Attach new tape to the empty roll by using the black sticky part.
202 -* Close tape stabilizer.
203 -
204 -**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
205 -**
206 -
207 207  \\
208 208  
209 209  \\
210 210  
211 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}14.6 Nozzle exchange ===
212 -
213 213  \\
214 214  
215 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Datacollection"/}}14.7 Data collection ===
216 -
217 217  \\
218 218  
219 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Troubleshooting"/}}14.8 Troubleshooting ===
220 -
221 -* (((
222 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
223 -)))
224 -* (((
225 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
226 -)))
227 -* (((
228 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
229 -)))
230 -
231 231  \\
232 -
233 -\\
Confluence.Code.ConfluencePageClass[0]
Id
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1 -236815972
1 +235039093
URL
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1 -https://confluence.desy.de/spaces/11/pages/236815972/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235039093/14. Serial Crystallography: CFEL TapeDrive