Changes for page CFEL TapeDrive: Serial Crystallography
Last modified by chatzies on 2025-11-27 11:35
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... ... @@ -1,1 +1,1 @@ 1 - 14. Serial Crystallography: CFEL TapeDrive1 +08. Serial Crystallography: CFEL TapeDrive - Author
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... ... @@ -1,1 +1,1 @@ 1 -XWiki.a henkel1 +XWiki.tabermah - Content
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... ... @@ -1,83 +1,32 @@ 1 1 {{layout}} 2 2 {{layout-section ac:type="single"}} 3 3 {{layout-cell}} 4 - 5 - 6 -{{toc/}} 7 - 8 8 [[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]] 9 9 10 -\\ 11 11 12 - \\7 +{{toc minLevel="2"/}} 13 13 14 -\\ 15 15 16 - === {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===10 +---- 17 17 18 -* remove vortex 19 -* beamstop to out-position? 20 -* remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01) 21 -* move cryojet out of the way, ramp the cryo up and shut it down. Close the compressed air to the shield flow. The biggest cable kinks and needs to be disconnected. 22 -* stop refill of the robot dewar and empty the dewar, warm up. 23 -* Disable backlight (unplug Festo connection). (% style="color: rgb(255,0,0);" %)//Is this still needed?// 24 -* (% style="color: rgb(0,0,0);" %)mount the 10 um diode and connect (via connector on the granite). This is connected to the leftmost Keithly (power up, check that settings are gain E06, Filter on, Rise time 1 ms. Keithly is connected to the oscilloscope and the delay generator, delay generator is connected to oscilloscope and detector, see diagram in P11 wiki. (% style="color: rgb(0, 0, 0); color: rgb(255, 0, 0)" %)//Insert here the setting for oscilloscope//(% style="color: rgb(0,0,0);" %)//. //Make sure to connect the multi-trigger to the back of the detector instead of the standard trigger, when starting to use the chopper (pull the thin ring with ‘grip’ area to detach). Cables are labeled. Make sure before using the chopper that the channels are enabled. 25 -* (% style="color: rgb(0,0,0);" %)move goniotower to X ~~ 17 000 12 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}**14.1 Sample characteristics and preparation** == 26 26 27 - (%style="color: rgb(0,0,0);" %)Mounting of the TapeDrive isdoneby the CFEL group.Electronics and control unitsare combined on a smallshelf,thatrequires only onepowerand networkplug-in.Theshelfwillbeplaced left-sided (looking upstream)to thedetectortower. TheElveFlowand controllaptop aswellasseveralnecessary consumablesare placedona cart,whichisto be positionedontheright(lookingupstream)against the robot platform.The CFEL chilleris placed ontherightof the platform andconnected to the TapeDrive GASnozzle.14 +suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc. 28 28 29 -\\ 30 - 31 -* adjust focus of the tape by moving X in goniometer tower until you see it in the overview camera. Final adjustment should be done when there is flow. Latest value is about 4500 in X and also 5000 in Z (higher soft limit changed from 2976,63 to 6000). (% style="color: rgb(255,0,0);" %)//Better to use the back illumination in the GUI to focus the tape - old instruction, not sure if this is the case. Back illumination is the external light source in the detector tower (the crystallography backlight should be disable for tapedrive).// 32 -* (% style="color: rgb(0,0,0);" %)Check the Minimum Detector Distance; the detector should not collide with the tapedrive while driven to 154 mm but it has to be verified. Also check that all cables and capillaries are taped away when the users start, so that nothing protrudes further than the tapedrive itself. Soft limit can be edited in eh.3.11 Atk panel. 33 -* (% style="color: rgb(0,0,0);" %)Reconfirm the alignment of the beamstop, once everything, including the nozzle, is in place. Modify config.ini for beamstop in- and out-position to both mach in (27 000). This way the beamstop will not be driven out when the hutch is opened, to avoid that the users forget to drive it in when restarting after entering the hutch. 34 - 35 -\\ 36 - 37 -Prepare necessary controls for data collection: 38 - 39 -* ATK panels: 40 -** p11/simplon_filewriter/eh.01 41 -** p11/simplon_detector/eh.01 42 -*** count time = 0.0075 43 -*** trigger (**ints **if working without the chopper, # or triggers is 1, **exts** if with the chopper, # or triggers = number of images). Exts is also the standard trigger in normal crystallography. If GUI is used to take images (e.g. for beamstop alignment) the trigger mode will be forced by that and needs to be reverted manually for the users to continue. 44 -*** numer of images = 100 000 45 -*** number of images in container = 1000 46 -*** mode 16m/4M 47 -*** detector distance = 200 mm (usually) 48 -** p11/detectortower 49 -** p11/motor/eh.09 50 -* remote access to TapeDrive control laptop: 51 -** open terminal, execute command: xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611 52 - password: //written on control laptop 53 -// 54 -* Cameras: 55 -** haspp11ethcam1 → detector tower camera to monitor nozzle 56 -** haspp11ethcam4 → goniometer camera to monitor tape from above 57 -* OnDa (on haspp11user04): 58 -//This starts both GUIs (Frame Viewer & Crystallography GUI) and onda-monitor, the server component. The tool has a bunch of parameters which you can list with ./onda-runner.py —help.//\\ 59 -** 16M mode → cd /home/p11user/CfelSoft/202103_onda 60 - ./onda-runner.py onda-config/onda-config-Eiger16M.toml 61 -** 4M mode → cd /home/p11user/CfelSoft/202103_onda 62 - ./onda-runner.py onda-config/onda-config-Eiger4M.toml\\ 63 - 64 -\\ 65 - 66 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** === 67 - 68 -suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc. 69 - 70 70 Preparation: 71 71 72 -* filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube 18 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube 73 73 74 74 [[image:attach:20211201_095534.jpg||height="250"]] 75 75 76 -\\ 77 77 78 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange 79 -** === 23 +---- 80 80 25 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}**14.2 Connecting sample to ElveFlow, sample exchange** == 26 + 27 +* 28 +** == 29 + 81 81 After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!** 82 82 83 83 Be aware to which channel and nozzle line your sample is connected. ... ... @@ -92,44 +92,47 @@ 92 92 93 93 [[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]] 94 94 95 -\\ 96 96 97 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation 98 -** === 45 +---- 99 99 100 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window. 47 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}**14.3 TapeDrive operation** == 48 + 49 +* 50 +** == 51 + 52 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window. 101 101 Now start the client. Same thing, a terminal will open, shortly after a small window. 102 102 103 103 Click 'Devices' in the **client session** to open the control window. 104 104 [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]] 105 105 106 -**Tape** 58 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ==== 107 107 108 -* Click onthe 'main tape' icon60 +* Click the 'main tape' icon 109 109 * Change the velocity by typing anything from **0.3-3.0 mm/s** 110 110 * Common velocity is 1.0-1.5 mm/s 111 111 * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point. 112 - \\64 + 113 113 * [[image:attach:kamzik_tape.PNG||height="400"]] 114 114 115 115 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ==== 116 116 117 -* Click an the 'nozzle' icon.69 +* Click on the 'nozzle' icon. 118 118 * ((( 119 119 The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully. 120 120 The absolute position is **not calibrated** and therefore changes after each restart of kamzik. 121 121 122 122 123 -(% style="color: rgb(255,0,0);" %)**!!! **(% style="color:rgb(255,0,0); color: rgb(0,0,0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:rgb(255,0,0);" %)**!!!**(%%)75 +(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%) 124 124 \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments. 125 125 126 126 (% class="wrapped" %) 127 127 |=((( 128 - \\80 + 129 129 )))|=((( 130 130 direction 131 131 )))|=((( 132 -recommended step size 84 +recommended step size 133 133 for visual adjustment 134 134 )))|=(% colspan="1" %)((( 135 135 recommended step size ... ... @@ -164,8 +164,7 @@ 164 164 be careful with -z, tape could be pushed out of focus 165 165 ))) 166 166 167 -** 168 -\\** 119 + 169 169 ))) 170 170 * [[image:attach:kamzik_nozzle.PNG||height="400"]] 171 171 ... ... @@ -173,28 +173,23 @@ 173 173 174 174 * The chiller circulates fluid through the gas nozzle. 175 175 176 -\\ 177 - 178 178 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ==== 179 179 180 -\\ 181 181 182 182 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ==== 183 183 184 -\\ 185 185 186 186 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command: 187 187 188 188 xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611 189 - password: //written on control laptop (changes every 6 months) 190 -// 136 + password: //written on control laptop (changes every 6 months)// 191 191 192 -\\ 193 193 194 -\\ 195 195 196 - === {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ===140 +---- 197 197 142 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}**14.4 Tape roll exchange** == 143 + 198 198 The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s). 199 199 200 200 * Remove the (almost) empty feeder roll by pulling it upwards. ... ... @@ -202,7 +202,7 @@ 202 202 * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm** 203 203 * Cut the first cm of the new tape roll, directly behind the black sticky part. 204 204 * Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll. 205 -* Open magnetic tape stabilizer on the **left side**. //!!!BEAMSTOP!!!// 151 +* Open magnetic tape stabilizer on the **left side**. (% style="color:#ff0000" %)//!!!BEAMSTOP!!!// 206 206 * Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s). 207 207 * Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded. 208 208 * Attach new tape to the empty roll by using some sticky tape. ... ... @@ -209,85 +209,112 @@ 209 209 * Close tape stabilizer. 210 210 * Reset tape counter in kamzik by clicking "<Call>" in "Main-Tape". 211 211 212 -**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]] 213 -** 158 +**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]** 214 214 215 -\\ 216 216 217 -\\ 218 218 219 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}**14.6 Nozzle exchange** === 162 + 163 +{{/layout-cell}} 164 +{{/layout-section}} 220 220 221 -\\ 166 +{{layout-section ac:type="single"}} 167 +{{layout-cell}} 168 +---- 222 222 223 -== ={{id name="14.SerialCrystallography:CFELTapeDrive-14.7Datacollection"/}}**14.7Data collection** ===170 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}**14.5 Chopper** == 224 224 225 - Once the hutch isinterlocked, setremotecontrol to the TapeDrivelaptopby opening aterminaland executing the following command:172 +start chopper gui from terminal: 226 226 227 - xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611 228 - password: //written on control laptop (changes every 6 months)// 174 +(% class="code" %) 175 +((( 176 + cd /gpfs/local/shared/ChopperControl 177 +))) 229 229 230 - //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password// 179 +(% class="code" %) 180 +((( 181 + python chopperControl.py 182 +\\ 183 +))) 184 +{{/layout-cell}} 185 +{{/layout-section}} 231 231 232 -\\ 187 +{{layout-section ac:type="two_equal"}} 188 +{{layout-cell}} 189 + [[image:attach:20211212_233140.jpg||height="250"]] 190 +{{/layout-cell}} 233 233 234 -**Check list:** 192 +{{layout-cell}} 193 +**Chopper → no Chopper** 235 235 236 -* Open BS1 (//CrystalControl)// 237 -* Put collimator in (//CrystalControl)// 238 -* Check beamstop position = 27 000 (//Atk panel p11/motor/eh.3.09)// 239 -* Open fast shutter (//CrystalControl)// 240 -* Check diode voltage ~~ 0.0 (//kamzik Run/Diode voltage)// 241 -* Close fast shutter (//CrystalControl)// 195 +* decrease frequency by max. 10 Hz steps 196 +** click arrow down 10x, "Set", arrow down 10x, "Set", .... 197 +* at frequency = 10 Hz, click "Stop" 198 +* set "Hole" to <none> (% style="color:#ff0000" %)//Takes some minutes// 242 242 243 - \\200 +(% style="color:#000000" %)**no Chopper → Chopper** 244 244 245 -**Start run in kamzik 246 -** 202 +* (% style="color:#000000" %)set "Hole" to <Inner hole (largest)> (% style="color:#ff0000" %)//Takes some minutes// 203 +* (% style="color:#000000" %)increase frequency by max. 10 Hz steps 204 +** (% style="color:#000000" %)click arrow up 10x, "Set", arrow up 10x, "Set", ..... 205 +* (% style="color:#000000" %)//usually 130 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 130 Hz// 206 +{{/layout-cell}} 207 +{{/layout-section}} 247 247 248 -Click "Start" icon in kamzik. 209 +{{layout-section ac:type="single"}} 210 +{{layout-cell}} 211 +---- 249 249 250 - [[image:attach:kamzik_run-tile.jpg||height="400"]]213 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** == 251 251 252 ----- 215 +Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command: 216 + 217 +(% class="code" %) 218 +((( 219 + xfreerdp +clipboard /size:1920x1080 /u:cfelusr /v:cfeld-tapetop 220 + password: written on control laptop (changes every 6 months) 221 +))) 222 + 223 + //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password// 224 + 225 + 253 253 {{/layout-cell}} 254 254 {{/layout-section}} 255 255 256 -{{layout-section ac:type="two_e qual"}}229 +{{layout-section ac:type="two_left_sidebar"}} 257 257 {{layout-cell}} 258 - \\231 +**Check list:** 259 259 260 -(% class="task-list" %) 261 -((( 262 -{{task reference="/Tasks/Task_16" status="InProgress"}} 263 -(% class="task-list" %) 264 -((( 265 -{{task reference="/Tasks/Task_22" status="InProgress"}} 266 -Set your desired No. of frames 267 -{{/task}} 233 +(% style="color:#ff0000" %)//always after entering the hutch!// 268 268 269 -{{task reference="/Tasks/Task_17" status="InProgress"}} 270 -Match exposure time with chopper setting 271 -{{/task}} 235 +(% style="color:#000000" %)Click "Run" icon in kamzik. 272 272 273 -{{task reference="/Tasks/Task_18" status="InProgress"}} 274 -Check trigger setting and match to chopper setting 275 -{{/task}} 237 +* Put detector distance 200 in //prepare_for_measurement ( )// 238 +* Click "<Call>" next to //prepare_for_measurement// 239 +This will: 240 +** drive the collimator in //IN// position 241 +** drive detector to 200 mm 276 276 277 -{{task reference="/Tasks/Task_19" status="InProgress"}} 278 -Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it. 279 -{{/task}} 243 +* Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed // 280 280 281 -{{task reference="/Tasks/Task_20" status="InProgress"}} 282 -To start the run, click "<Call>" next to //exposure time// 283 -{{/task}} 284 -))) 285 -{{/task}} 286 -))) 245 +//**Set run parameters and go 🏃💥**// 246 + 247 +1. Set your desired No. of frames 248 +1. Enter the protein ID of your crystal sample 249 +1. Match exposure time with chopper setting or set desired exposure time 250 +1. Check trigger setting and match to chopper setting 251 +no chopper → external triffer //off// 252 + chopper → external trigger// on// 253 +1. To start the run, click "<Call>" next to //start_run (exposure )// 287 287 {{/layout-cell}} 288 288 289 289 {{layout-cell}} 290 -(% class="wrapped" %) 257 +[[image:attach:Kamzik_run(2).PNG]] 258 +{{/layout-cell}} 259 +{{/layout-section}} 260 + 261 +{{layout-section ac:type="single"}} 262 +{{layout-cell}} 263 +(% class="relative-table wrapped" style="width:55.9569%" %) 291 291 |=((( 292 292 Parameter 293 293 )))|=((( ... ... @@ -298,7 +298,7 @@ 298 298 |((( 299 299 Images number 300 300 )))|((( 301 - Setnumber of frames you want to collect274 +number of frames you want to collect 302 302 )))|(% colspan="1" %)((( 303 303 usually 100k - 200k, but adjust according to hitrate 304 304 ... ... @@ -309,12 +309,9 @@ 309 309 )))|((( 310 310 exposure time of Eiger 311 311 312 - \\285 + 313 313 )))|(% colspan="1" %)((( 314 -chopper at 133 Hz → 3.7 ms 315 - 316 -chopper at 66 Hz → 7.7 ms 317 - 287 +with chopper → opening time displayed in chopper GUI 318 318 no chopper → 7.7 ms 319 319 320 320 ---- ... ... @@ -324,7 +324,7 @@ 324 324 )))|((( 325 325 changes automatically 326 326 )))|(% colspan="1" %)((( 327 - \\297 + 328 328 ))) 329 329 |(% colspan="1" %)((( 330 330 Detector distance ... ... @@ -331,38 +331,109 @@ 331 331 )))|(% colspan="1" %)((( 332 332 current detector postion readout 333 333 )))|(% colspan="1" %)((( 334 - \\304 + 335 335 ))) 336 336 |(% colspan="1" %)((( 337 - move_detector (distance)307 +protein ID 338 338 )))|(% colspan="1" %)((( 339 - Setdetectordistance309 +protein ID of crystal sample (see AMARCORD) 340 340 )))|(% colspan="1" %)((( 341 - 200.0mm (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"311 + 342 342 ))) 343 -|(% colspan="1" %)((( 344 -Diode voltage 345 -)))|(% colspan="1" %)((( 346 -read out of diode 347 -)))|(% colspan="1" %)((( 348 -**~~ 0 !!! higher values indicate missaligned beam stop!!!** 349 -))) 313 + 314 +---- 350 350 {{/layout-cell}} 351 351 {{/layout-section}} 352 352 318 +{{layout-section ac:type="two_equal"}} 319 +{{layout-cell}} 320 +**After data collection/Before entering the exp. hutch:** 321 + 322 +1. Go to "Safety" in kamzik. 323 +1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically: 324 +11. Drive the collimator out 325 +11. Drive the detector back to 1500 mm 326 +1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left). 327 + 328 +[[image:attach:Screenshot from 2022-04-04 19-36-29.png]] 329 +{{/layout-cell}} 330 + 331 +{{layout-cell}} 332 + 333 + 334 + 335 + 336 + 337 + 338 +[[image:attach:safety_kamzik.PNG]] 339 +{{/layout-cell}} 340 +{{/layout-section}} 341 + 353 353 {{layout-section ac:type="single"}} 354 354 {{layout-cell}} 355 - === {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Troubleshooting"/}}14.8 Troubleshooting ===344 +---- 356 356 346 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}**14.7 Troubleshooting** == 347 + 357 357 * ((( 358 358 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==== 350 + 351 +* Tape roll empty? 352 +* motor connected (icon green)? 359 359 ))) 354 + 360 360 * ((( 361 361 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ==== 357 + 358 +* displayed in green=connected? 359 +* nozzle holder colliding somewhere? 360 +* motor limits reached? → position displayed in red 362 362 ))) 362 + 363 363 * ((( 364 364 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ==== 365 + 366 +* channel turned on? 367 +* Sample empty? 368 +* Nozzle or lines clogged? → flush with water, change nozzle 365 365 ))) 370 + 371 +* ((( 372 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ==== 373 + 374 +* First, restart kamzik (server+client) and see if this solves the issue 375 +* If not, restart the Simplon server (in Astor → haspp11exp03, right click on Simplon/EH.1 and restart) 376 +* ((( 377 +If nothing works, kill the Simplon process and then start the server again:* 378 + 379 +((( 380 +(% class="code" %) 381 +((( 382 +ssh haspp11exp03 383 +))) 384 +))) 385 + 386 +* ((( 387 +(% class="code" %) 388 +((( 389 +ps -ef | grep Simplon 390 +))) 391 +))) 392 +* ((( 393 +(% class="code" %) 394 +((( 395 +kill -9 simplon_process_id 396 +))) 397 +))) 398 +))) 399 +* After the Simplon server is back up, close all kamzik windows and restart (first server, then client) 400 +))) 366 366 {{/layout-cell}} 367 367 {{/layout-section}} 403 + 404 +{{layout-section ac:type="single"}} 405 +{{layout-cell}} 406 + 407 +{{/layout-cell}} 408 +{{/layout-section}} 368 368 {{/layout}}
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