Changes for page CFEL TapeDrive: Serial Crystallography

Last modified by chatzies on 2025-11-27 11:35

From 27.1 to 26.1 From 44.1 to 43.1

From version 43.1

edited by ahenkel
on 2021-12-12 23:49

Change comment: There is no comment for this version

To version 27.1

edited by ahenkel
on 2021-12-05 19:34

Change comment: There is no comment for this version

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@@ -1,6 +1,7 @@
--{{layout}}
--{{layout-section ac:type="single"}}
--{{layout-cell}}
++
++
++{{toc/}}
++
  [[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
  \\
@@ -78,16 +78,14 @@
  Be aware to which channel and nozzle line your sample is connected.
--            Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
++            Red → Channel 1, crystal line (nozzle capillary without marks)
              Green → Channel 2, substrate line (nozzle capillary is marked in black)
  [[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
--After finishing data collection, flush all lines by connecting an Eppi or syringe filled with ddH,,2,,O.
++\\
--[[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]]
--
  \\
  === {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
@@ -114,11 +114,10 @@
  * (((
  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
++\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
++\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
--
--(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
--\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
--
++(% class="wrapped" %)
  |=(((
  \\
  )))|=(((
@@ -166,16 +166,12 @@
  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
--* The chiller circulates fluid through the gas nozzle.
++\\
  \\
--==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ====
--
  \\
--==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ====
--
  \\
  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
@@ -195,16 +195,15 @@
  * Remove the (almost) empty feeder roll by pulling it upwards.
  * Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
  * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
--* Cut the first cm of the new tape roll, directly behind the black sticky part.
--* Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll.
--* Open magnetic tape stabilizer on the **left side**. //!!!BEAMSTOP!!!//
--* Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s).
--* Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded.
--* Attach new tape to the empty roll by using some sticky tape.
++* Cut the first cm of the new tape roll, directly before the black sticky part.
++* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
++* Open magnetic tape stabilizer.
++* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
++* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
++* Attach new tape to the empty roll by using the black sticky part.
  * Close tape stabilizer.
--* Reset tape counter in kamzik by clicking "<Call>" in "Main-Tape".
--**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]
++**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
  **
  \\
@@ -211,212 +211,8 @@
  \\
--=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}**14.6 Nozzle exchange** ===
--{{/layout-cell}}
--{{/layout-section}}
--
--{{layout-section ac:type="single"}}
--{{layout-cell}}
--=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Chopper"/}}**14.7 Chopper** ===
--
--start chopper gui from terminal:
--
--(% class="code" %)
--(((
--            cd /gpfs/local/shared/ChopperControl
--)))
--
--(% class="code" %)
--(((
--            python chopperControl.py
--)))
--{{/layout-cell}}
--{{/layout-section}}
--
--{{layout-section ac:type="two_equal"}}
--{{layout-cell}}
--                     [[image:attach:20211212_233140.jpg||height="250"]]
--{{/layout-cell}}
--
--{{layout-cell}}
--**Chopper → no Chopper**
--
--* decrease frequency by max. 10 Hz steps\\
--** click arrow down 10x, "Set", arrow down 10x, "Set", ....
--* at frequency = 10 Hz, click "Stop"
--* set "Hole" to <none> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
--
--(% style="color: rgb(0,0,0);" %)**no Chopper → Chopper**
--
--* (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
--* (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps
--** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", .....
--* (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms//
--{{/layout-cell}}
--{{/layout-section}}
--
--{{layout-section ac:type="single"}}
--{{layout-cell}}
--=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Datacollection"/}}**14.8 Data collection** ===
--
--Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
--
--(% class="code" %)
--(((
--                      xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
--                      password: written on control laptop (changes every 6 months)
--)))
--
--                                           //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
--
  \\
--Click "Start" icon in kamzik.
--
--[[image:attach:kamzik_run-tile.jpg||height="400"]]
--
------
--{{/layout-cell}}
--{{/layout-section}}
--
--{{layout-section ac:type="two_equal"}}
--{{layout-cell}}
--**Check list:**
--
--(% style="color: rgb(255,0,0);" %)//always after entering the hutch!//
--
--* Open BS1  (//CrystalControl)//
--* Put collimator in (//CrystalControl)//
--* Check beamstop position = 27 000 (//Atk panel p11/motor/eh.3.09)//
--* Open fast shutter (//CrystalControl)//
--* Check diode voltage ~~ 0.0 (//kamzik Run/Diode voltage)//
--* Close fast shutter (//CrystalControl)//
--
  \\
--**Set run parameters and go 🏃💥**
--
--1. Set your desired No. of frames
--1. Match exposure time with chopper setting
--1. Check trigger setting and match to chopper setting
--1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
--1. To start the run, click "<Call>" next to //exposure time//
--
  \\
--
--(% style="color: rgb(153,51,102);" %)**//Current bug~://**
--
--(% style="color: rgb(0,0,0);" %)You need to "Stop" run in kamzik manually after all images have been acquired. This moves the shield up and closes the fast shutter. Afterwards you can start a new run by clicking "<Call>".
--
--
--(% style="color: rgb(0,0,0);" %)Check progress in terminal with:
--
--(% class="code" %)
--(((
--      cd /gpfs/current/raw/scan_frames
--      watch "ls -lrt <run number>/ | tail"
--\\\\\\\\
--)))
--{{/layout-cell}}
--
--{{layout-cell}}
--(% class="wrapped relative-table" style="width: 100.0%;" %)
--|=(((
--Parameter
--)))|=(((
--explanation
--)))|=(% colspan="1" %)(((
--value
--)))
--|(((
--Images number
--)))|(((
--Set number of frames you want to collect
--)))|(% colspan="1" %)(((
--usually 100k - 200k, but adjust according to hitrate
--
--orientation: 10 % hitrate needs at least 100k images
--)))
--|(((
--exposure
--)))|(((
--exposure time of Eiger
--
--\\
--)))|(% colspan="1" %)(((
--chopper at 133 Hz → 3.7 ms
--
--chopper at 66 Hz   → 7.7 ms
--
--no chopper            → 7.7 ms
--
------
--)))
--|(((
--run id
--)))|(((
--changes automatically
--)))|(% colspan="1" %)(((
--\\
--)))
--|(% colspan="1" %)(((
--Detector distance
--)))|(% colspan="1" %)(((
--current detector postion readout
--)))|(% colspan="1" %)(((
--\\
--)))
--|(% colspan="1" %)(((
--move_detector (distance)
--)))|(% colspan="1" %)(((
--Set detector distance
--)))|(% colspan="1" %)(((
--200.0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
--)))
--|(% colspan="1" %)(((
--Diode voltage
--)))|(% colspan="1" %)(((
--read out of diode
--)))|(% colspan="1" %)(((
--**~~ 0   !!! higher values indicate missaligned beam stop!!!
--Call local contact
--**
--)))
--{{/layout-cell}}
--{{/layout-section}}
--
--{{layout-section ac:type="single"}}
--{{layout-cell}}
------
--
--=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.9Troubleshooting"/}}14.9 Troubleshooting ===
--
--* (((
--==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
--
--* Tape roll empty?
--* motor connected (icon green)?
--)))
--
--\\
--
--* (((
--==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
--
--* displayed in green=connected?
--* nozzle holder colliding somewhere?
--* motor limits reached? → position displayed in red
--)))
--
--\\
--
--* (((
--==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
--
--* channel turned on?
--* Sample empty?
--* Nozzle or lines clogged? → flush with water, change nozzle
--)))
--{{/layout-cell}}
--{{/layout-section}}
--{{/layout}}

Confluence.Code.ConfluencePageClass[0]

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@@ -1,1 +1,1 @@
--236824590
++235041518

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@@ -1,1 +1,1 @@
--https://confluence.desy.de/spaces/11/pages/236824590/14. Serial Crystallography: CFEL TapeDrive
++https://confluence.desy.de/spaces/11/pages/235041518/14. Serial Crystallography: CFEL TapeDrive

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