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Last modified by chatzies on 2025-11-27 11:35
From version 43.1
edited by ahenkel
on 2021-12-12 23:49
Change comment: There is no comment for this version
To version 45.1
edited by ahenkel
on 2021-12-12 23:53
Change comment: There is no comment for this version

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1 1  {{layout}}
2 2  {{layout-section ac:type="single"}}
3 3  {{layout-cell}}
4 -[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
4 +[[image:attach:TapeDrive_beschriftet-tile.jpg||height="250"]]
5 5  
6 6  \\
7 7  
8 8  \\
9 9  
10 -\\
10 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}**14.1 Sample characteristics and preparation** ==
11 11  
12 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
13 -
14 -* remove vortex
15 -* beamstop to out-position?
16 -* remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
17 -* move cryojet out of the way, ramp the cryo up and shut it down. Close the compressed air to the shield flow. The biggest cable kinks and needs to be disconnected.
18 -* stop refill of the robot dewar and empty the dewar, warm up.
19 -* Disable backlight (unplug Festo connection). (% style="color: rgb(255,0,0);" %)//Is this still needed?//
20 -* (% style="color: rgb(0,0,0);" %)mount the 10 um diode and connect (via connector on the granite). This is connected to the leftmost Keithly (power up, check that settings are gain E06, Filter on, Rise time 1 ms. Keithly is connected to the oscilloscope and the delay generator, delay generator is connected to oscilloscope and detector, see diagram in P11 wiki. (% style="color: rgb(0, 0, 0); color: rgb(255, 0, 0)" %)//Insert here the setting for oscilloscope//(% style="color: rgb(0,0,0);" %)//. //Make sure to connect the multi-trigger to the back of the detector instead of the standard trigger, when starting to use the chopper (pull the thin ring with ‘grip’ area to detach). Cables are labeled. Make sure before using the chopper that the channels are enabled.
21 -* (% style="color: rgb(0,0,0);" %)move goniotower to X ~~ 17 000
22 -
23 -(% style="color: rgb(0,0,0);" %)Mounting of the TapeDrive is done by the CFEL group. Electronics and control units are combined on a small shelf, that requires only one power and network plug-in. The shelf will be placed left-sided (looking upstream) to the detector tower. The ElveFlow and control laptop as well as several necessary consumables are placed on a cart, which is to be positioned on the right (looking upstream) against the robot platform. The CFEL chiller is placed on the right of the platform and connected to the TapeDrive GASnozzle.
24 -
25 -\\
26 -
27 -* adjust focus of the tape by moving X in goniometer tower until you see it in the overview camera. Final adjustment should be done when there is flow. Latest value is about 4500 in X and also 5000 in Z (higher soft limit changed from 2976,63 to 6000). (% style="color: rgb(255,0,0);" %)//Better to use the back illumination in the GUI to focus the tape - old instruction, not sure if this is the case. Back illumination is the external light source in the detector tower (the crystallography backlight should be disable for tapedrive).//
28 -* (% style="color: rgb(0,0,0);" %)Check the Minimum Detector Distance; the detector should not collide with the tapedrive while driven to 154 mm but it has to be verified. Also check that all cables and capillaries are taped away when the users start, so that nothing protrudes further than the tapedrive itself. Soft limit can be edited in eh.3.11 Atk panel.
29 -* (% style="color: rgb(0,0,0);" %)Reconfirm the alignment of the beamstop, once everything, including the nozzle, is in place. Modify config.ini for beamstop in- and out-position to both mach in (27 000). This way the beamstop will not be driven out when the hutch is opened, to avoid that the users forget to drive it in when restarting after entering the hutch.
30 -
31 -\\
32 -
33 -Prepare necessary controls for data collection:
34 -
35 -* ATK panels:
36 -** p11/simplon_filewriter/eh.01
37 -** p11/simplon_detector/eh.01
38 -*** count time = 0.0075
39 -*** trigger (**ints **if working without the chopper, # or triggers is 1, **exts** if with the chopper, # or triggers = number of images). Exts is also the standard trigger in normal crystallography. If GUI is used to take images (e.g. for beamstop alignment) the trigger mode will be forced by that and needs to be reverted manually for the users to continue.
40 -*** numer of images = 100 000
41 -*** number of images in container = 1000
42 -*** mode 16m/4M
43 -*** detector distance = 200 mm (usually)
44 -** p11/detectortower
45 -** p11/motor/eh.09
46 -* remote access to TapeDrive control laptop:
47 -** open terminal, execute command:          xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
48 - password: //written on control laptop
49 -//
50 -* Cameras:
51 -** haspp11ethcam1 → detector tower camera to monitor nozzle
52 -** haspp11ethcam4 →  goniometer camera to monitor tape from above
53 -* OnDa (on haspp11user04): 
54 -//This starts both GUIs (Frame Viewer & Crystallography GUI) and onda-monitor, the server component. The tool has a bunch of parameters which you can list with ./onda-runner.py —help.//\\
55 -** 16M mode →    cd /home/p11user/CfelSoft/202103_onda
56 - ./onda-runner.py onda-config/onda-config-Eiger16M.toml
57 -** 4M mode   →    cd /home/p11user/CfelSoft/202103_onda
58 - ./onda-runner.py onda-config/onda-config-Eiger4M.toml\\
59 -
60 -\\
61 -
62 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
63 -
64 64  suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
65 65  
66 66  Preparation:
... ... @@ -71,8 +71,8 @@
71 71  
72 72  \\
73 73  
74 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
75 -** ===
22 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}**14.2 Connecting sample to ElveFlow, sample exchange
23 +** ==
76 76  
77 77  After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
78 78  
... ... @@ -90,8 +90,8 @@
90 90  
91 91  \\
92 92  
93 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
94 -** ===
41 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}**14.3 TapeDrive operation
42 +** ==
95 95  
96 96  The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
97 97  Now start the client. Same thing, a terminal will open, shortly after a small window.
... ... @@ -99,7 +99,7 @@
99 99  Click 'Devices' in the **client session** to open the control window.
100 100  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
101 101  
102 -**Tape**
50 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
103 103  
104 104  * Click on the 'main tape' icon
105 105  * Change the velocity by typing anything from **0.3-3.0 mm/s**
... ... @@ -119,6 +119,7 @@
119 119  (% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
120 120  \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
121 121  
70 +(% class="wrapped" %)
122 122  |=(((
123 123  \\
124 124  )))|=(((
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188 188  
189 189  \\
190 190  
191 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ===
140 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ==
192 192  
193 193  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
194 194  
... ... @@ -211,13 +211,14 @@
211 211  
212 212  \\
213 213  
214 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}**14.6 Nozzle exchange** ===
163 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-"/}}**
164 +** ===
215 215  {{/layout-cell}}
216 216  {{/layout-section}}
217 217  
218 218  {{layout-section ac:type="single"}}
219 219  {{layout-cell}}
220 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Chopper"/}}**14.7 Chopper** ===
170 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Chopper"/}}**14.7 Chopper** ==
221 221  
222 222  start chopper gui from terminal:
223 223  
... ... @@ -257,7 +257,7 @@
257 257  
258 258  {{layout-section ac:type="single"}}
259 259  {{layout-cell}}
260 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Datacollection"/}}**14.8 Data collection** ===
210 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Datacollection"/}}**14.8 Data collection** ==
261 261  
262 262  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
263 263  
... ... @@ -389,7 +389,7 @@
389 389  {{layout-cell}}
390 390  ----
391 391  
392 -=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.9Troubleshooting"/}}14.9 Troubleshooting ===
342 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.9Troubleshooting"/}}**14.9 Troubleshooting** ==
393 393  
394 394  * (((
395 395  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
Confluence.Code.ConfluencePageClass[0]
Id
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1 -236824590
1 +236824595
URL
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1 -https://confluence.desy.de/spaces/11/pages/236824590/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/236824595/14. Serial Crystallography: CFEL TapeDrive