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Last modified by chatzies on 2025-11-27 11:35
From version 5.1
edited by ahenkel
on 2021-11-30 16:03
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To version 30.1
edited by ahenkel
on 2021-12-07 17:44
Change comment: There is no comment for this version

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1 -**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)**
2 2  
2 +
3 +{{toc/}}
4 +
5 +[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
6 +
7 +\\
8 +
9 +\\
10 +
11 +\\
12 +
13 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
14 +
3 3  * remove vortex
4 4  * beamstop to out-position?
5 5  * remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
... ... @@ -34,7 +34,8 @@
34 34  ** p11/motor/eh.09
35 35  * remote access to TapeDrive control laptop:
36 36  ** open terminal, execute command:          xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
37 - password: Sp!ceM3lange
49 + password: //written on control laptop
50 +//
38 38  * Cameras:
39 39  ** haspp11ethcam1 → detector tower camera to monitor nozzle
40 40  ** haspp11ethcam4 →  goniometer camera to monitor tape from above
... ... @@ -47,20 +47,174 @@
47 47  
48 48  \\
49 49  
50 -**14.2 Sample characteristics and preparation**
63 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
51 51  
52 -suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **plus **few mL of mother liquor extra
65 +suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
53 53  
54 54  Preparation:
55 55  
56 56  * filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
57 57  
58 -[[image:url:https://www.sysmex.de/fileadmin/_processed_/a/d/csm_Sysmex_Partec_celltrics_filter_frei_20cacc97b9.png||width="500" height="334"]]
71 +[[image:attach:20211201_095534.jpg||height="250"]]
59 59  
60 60  \\
61 61  
75 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
76 +** ===
77 +
78 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
79 +
80 +Be aware to which channel and nozzle line your sample is connected.
81 +
82 + Red → Channel 1, crystal line (nozzle capillary without marks)
83 +
84 + Green → Channel 2, substrate line (nozzle capillary is marked in black)
85 +
86 +[[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
87 +
88 +After finishing data collection, flush all lines by connecting an Eppi or syringe filled with ddH,,2,,O.
89 +
90 +[[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]]
91 +
62 62  \\
63 63  
94 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
95 +** ===
96 +
97 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
98 +Now start the client. Same thing, a terminal will open, shortly after a small window.
99 +
100 +Click 'Devices' in the **client session** to open the control window.
101 +[[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
102 +
103 +**Tape**
104 +
105 +* Click on the 'main tape' icon
106 +* Change the velocity by typing anything from **0.3-3.0 mm/s**
107 +* Common velocity is 1.0-1.5 mm/s
108 +* kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
64 64  \\
110 +* [[image:attach:kamzik_tape.PNG||height="400"]]
65 65  
112 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
113 +
114 +* Click an the 'nozzle' icon.
115 +* (((
116 +The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
117 +The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
118 +\\(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
119 +\\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
120 +
121 +(% class="wrapped" %)
122 +|=(((
66 66  \\
124 +)))|=(((
125 +direction
126 +)))|=(((
127 +recommended step size
128 +for visual adjustment
129 +)))|=(% colspan="1" %)(((
130 +recommended step size
131 +during data collection
132 +)))
133 +|(((
134 ++x
135 +)))|(((
136 +away from X-ray interaction point
137 +)))|(((
138 +1.0 - 0.5 mm
139 +)))|(% colspan="1" %)(((
140 +0.1 - 0.2 mm
141 +)))
142 +|(((
143 ++y
144 +)))|(((
145 +up
146 +)))|(((
147 +1.0 - 0.5 mm
148 +)))|(% colspan="1" %)(((
149 +0.1 - 0.2 mm
150 +)))
151 +|(((
152 ++z
153 +)))|(((
154 +towards detector tower, away from tape
155 +)))|(((
156 +0.5 - 0.2 mm
157 +)))|(% colspan="1" %)(((
158 +0.1 - 0.2 mm
159 +be careful with -z, tape could be pushed out of focus
160 +)))
161 +
162 +**
163 +\\**
164 +)))
165 +* [[image:attach:kamzik_nozzle.PNG||height="400"]]
166 +
167 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
168 +
169 +* The chiller circulates fluid through the gas\\
170 +
171 +\\
172 +
173 +\\
174 +
175 +\\
176 +
177 +\\
178 +
179 +Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
180 +
181 + xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
182 + password: //written on control laptop (changes every 6 months)
183 +//
184 +
185 +\\
186 +
187 +\\
188 +
189 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ===
190 +
191 +The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
192 +
193 +* Remove the (almost) empty feeder roll by pulling it upwards.
194 +* Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
195 +* Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
196 +* Cut the first cm of the new tape roll, directly before the black sticky part.
197 +* Use this sticky part to attach the end of the old tape to the new roll. Place new roll on roll holder.
198 +* Open magnetic tape stabilizer.
199 +* Start the tape (3mm/s). Once the connetion side (black sticky part) has arrived at the collector roll, stop the tape (0mm/s).
200 +* Again, cut the tape directly in front of the black mark. Replace the the old roll with the **empty **one. Old roll can be discarded.
201 +* Attach new tape to the empty roll by using the black sticky part.
202 +* Close tape stabilizer.
203 +
204 +**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]]
205 +**
206 +
207 +\\
208 +
209 +\\
210 +
211 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}14.6 Nozzle exchange ===
212 +
213 +\\
214 +
215 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Datacollection"/}}14.7 Data collection ===
216 +
217 +\\
218 +
219 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Troubleshooting"/}}14.8 Troubleshooting ===
220 +
221 +* (((
222 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
223 +)))
224 +* (((
225 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
226 +)))
227 +* (((
228 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
229 +)))
230 +
231 +\\
232 +
233 +\\
Confluence.Code.ConfluencePageClass[0]
Id
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1 -235034275
1 +236815972
URL
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1 -https://confluence.desy.de/spaces/11/pages/235034275/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/236815972/14. Serial Crystallography: CFEL TapeDrive