Changes for page CFEL TapeDrive: Serial Crystallography

Last modified by chatzies on 2025-11-27 11:35

From 52.1 to 53.1 From 69.1 to 70.1

From version 53.1

edited by ahenkel
on 2022-04-04 19:58

Change comment: There is no comment for this version

To version 69.1

edited by ahenkel
on 2022-05-07 18:59

Change comment: There is no comment for this version

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@@ -1,7 +1,7 @@
  {{layout}}
  {{layout-section ac:type="single"}}
  {{layout-cell}}
--[[image:attach:TapeDrive_beschriftet-tile.jpg||height="250"]]
++[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
  \\
@@ -13,11 +13,11 @@
  == {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}**14.1 Sample characteristics and preparation** ==
--suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
++suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
  Preparation:
--* filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
++* filter through 30 µm cell filter (green) or 50 µm (yellow)   into Eppendorf or Falcon (original!) tube
  [[image:attach:20211201_095534.jpg||height="250"]]
@@ -57,7 +57,7 @@
  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
--* Click on the 'main tape' icon
++* Click the 'main tape' icon
  * Change the velocity by typing anything from **0.3-3.0 mm/s**
  * Common velocity is 1.0-1.5 mm/s
  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
@@ -66,7 +66,7 @@
  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
--* Click an the 'nozzle' icon.
++* Click on the 'nozzle' icon.
  * (((
  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
@@ -156,7 +156,7 @@
  * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
  * Cut the first cm of the new tape roll, directly behind the black sticky part.
  * Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll.
--* Open magnetic tape stabilizer on the **left side**. //!!!BEAMSTOP!!!//
++* Open magnetic tape stabilizer on the **left side**. (% style="color: rgb(255,0,0);" %)//!!!BEAMSTOP!!!//
  * Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s).
  * Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded.
  * Attach new tape to the empty roll by using some sticky tape.
@@ -214,12 +214,15 @@
  * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
  * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps
  ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", .....
--* (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms//
++* (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 133 Hz
++//
  {{/layout-cell}}
  {{/layout-section}}
--{{layout-section ac:type="two_equal"}}
++{{layout-section ac:type="single"}}
  {{layout-cell}}
++----
++
  == {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** ==
  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
@@ -233,65 +233,44 @@
                                 //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
  \\
++{{/layout-cell}}
++{{/layout-section}}
++{{layout-section ac:type="two_left_sidebar"}}
++{{layout-cell}}
  **Check list:**
  (% style="color: rgb(255,0,0);" %)//always after entering the hutch!//
--(% style="color: rgb(0,0,0);" %)Click "Safety" icon in kamzik.
++(% style="color: rgb(0,0,0);" %)Click "Run" icon in kamzik.
  * Put collimator in
--** Click "<Call>" next to //collimator_in//
++** Put detector distance 200 in //prepare_for_measurement (      )
++//
++** Click "<Call>" next to //prepare_for_measurement//
  \\
--* Open beam shutter BS1 in //CrystalControl//
--{{/layout-cell}}
++* Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed //
--{{layout-cell}}
--[[image:attach:kamzik_safety.PNG||height="400"]]
--{{/layout-cell}}
--{{/layout-section}}
++\\
--{{layout-section ac:type="single"}}
--{{layout-cell}}
------
++//**Set run parameters and go 🏃💥**//
--Click "Start/Run" icon in kamzik.
++1. Set your desired No. of frames
++1. Match exposure time with chopper setting or set desired exposure time
++1. Check trigger setting and match to chopper setting
++1. To start the run, click "<Call>" next to //start_run (exposure    )//
++{{/layout-cell}}
--[[image:attach:kamzik_run-tile.jpg||height="400"]]
--
------
++{{layout-cell}}
++[[image:attach:Kamzik_run(2).PNG]]
  {{/layout-cell}}
  {{/layout-section}}
  {{layout-section ac:type="two_equal"}}
  {{layout-cell}}
--**Set run parameters and go 🏃💥**
--
--1. Set your desired No. of frames
--1. Match exposure time with chopper setting
--1. Check trigger setting and match to chopper setting
--1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
--1. To start the run, click "<Call>" next to //exposure time//
--
--\\
--
--\\
--
--**After data collection/Before entering the exp. hutch:**
--
--1. Go to "Safety" in kamzik.
--1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
--11. Drive the collimator out
--11. Drive the detector back to 1500 mm
--1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
--
--[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]]
--{{/layout-cell}}
--
--{{layout-cell}}
--(% class="wrapped relative-table" style="width: 100.0%;" %)
++(% class="relative-table" %)
  |=(((
  Parameter
  )))|=(((
@@ -315,7 +315,7 @@
  \\
  )))|(% colspan="1" %)(((
--chopper at 133 Hz → 3.7 ms
++chopper at 133 Hz → 7.7 ms (opening time will be 3.7)
  chopper at 66 Hz   → 7.7 ms
@@ -344,18 +344,33 @@
  )))|(% colspan="1" %)(((
 .0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
  )))
--|(% colspan="1" %)(((
--Diode voltage
--)))|(% colspan="1" %)(((
--read out of diode
--)))|(% colspan="1" %)(((
--**~~ 0   !!! higher values indicate missaligned beam stop!!!
--Call local contact
--**
--)))
  {{/layout-cell}}
++
++{{layout-cell}}
++\\
++{{/layout-cell}}
  {{/layout-section}}
++{{layout-section ac:type="two_equal"}}
++{{layout-cell}}
++**After data collection/Before entering the exp. hutch:**
++
++1. Go to "Safety" in kamzik.
++1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
++11. Drive the collimator out
++11. Drive the detector back to 1500 mm
++1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
++
++[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]]
++{{/layout-cell}}
++
++{{layout-cell}}
++[[image:attach:safety_kamzik.PNG]]
++
++\\
++{{/layout-cell}}
++{{/layout-section}}
++
  {{layout-section ac:type="single"}}
  {{layout-cell}}
  ----

Confluence.Code.ConfluencePageClass[0]

Id

@@ -1,1 +1,1 @@
--250178641
++254729371

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@@ -1,1 +1,1 @@
--https://confluence.desy.de/spaces/11/pages/250178641/14. Serial Crystallography: CFEL TapeDrive
++https://confluence.desy.de/spaces/11/pages/254729371/14. Serial Crystallography: CFEL TapeDrive

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