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Last modified by chatzies on 2025-11-27 11:35
From version 55.1
edited by ahenkel
on 2022-04-04 19:58
Change comment: There is no comment for this version
To version 68.1
edited by ahenkel
on 2022-05-07 18:53
Change comment: There is no comment for this version

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... ... @@ -17,7 +17,7 @@
17 17  
18 18  Preparation:
19 19  
20 -* filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
20 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
21 21  
22 22  [[image:attach:20211201_095534.jpg||height="250"]]
23 23  
... ... @@ -57,7 +57,7 @@
57 57  
58 58  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
59 59  
60 -* Click on the 'main tape' icon
60 +* Click the 'main tape' icon
61 61  * Change the velocity by typing anything from **0.3-3.0 mm/s**
62 62  * Common velocity is 1.0-1.5 mm/s
63 63  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
... ... @@ -66,7 +66,7 @@
66 66  
67 67  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
68 68  
69 -* Click an the 'nozzle' icon.
69 +* Click on the 'nozzle' icon.
70 70  * (((
71 71  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
72 72  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
... ... @@ -156,7 +156,7 @@
156 156  * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
157 157  * Cut the first cm of the new tape roll, directly behind the black sticky part.
158 158  * Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll.
159 -* Open magnetic tape stabilizer on the **left side**. //!!!BEAMSTOP!!!//
159 +* Open magnetic tape stabilizer on the **left side**. (% style="color: rgb(255,0,0);" %)//!!!BEAMSTOP!!!//
160 160  * Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s).
161 161  * Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded.
162 162  * Attach new tape to the empty roll by using some sticky tape.
... ... @@ -214,11 +214,12 @@
214 214  * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
215 215  * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps
216 216  ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", .....
217 -* (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms//
217 +* (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 133 Hz
218 +//
218 218  {{/layout-cell}}
219 219  {{/layout-section}}
220 220  
221 -{{layout-section ac:type="two_equal"}}
222 +{{layout-section ac:type="single"}}
222 222  {{layout-cell}}
223 223  == {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** ==
224 224  
... ... @@ -233,65 +233,44 @@
233 233   //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
234 234  
235 235  \\
237 +{{/layout-cell}}
238 +{{/layout-section}}
236 236  
240 +{{layout-section ac:type="two_left_sidebar"}}
241 +{{layout-cell}}
237 237  **Check list:**
238 238  
239 239  (% style="color: rgb(255,0,0);" %)//always after entering the hutch!//
240 240  
241 -(% style="color: rgb(0,0,0);" %)Click "Safety" icon in kamzik.
246 +(% style="color: rgb(0,0,0);" %)Click "Run" icon in kamzik.
242 242  
243 243  * Put collimator in
244 -** Click "<Call>" next to //collimator_in//
249 +** Put detector distance 200 in //prepare_for_measurement (      )
250 +//
251 +** Click "<Call>" next to //prepare_for_measurement//
245 245  
246 246  \\
247 247  
248 -* Open beam shutter BS1 in //CrystalControl//
249 -{{/layout-cell}}
255 +* Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed //
250 250  
251 -{{layout-cell}}
252 -[[image:attach:kamzik_safety.PNG||height="400"]]
253 -{{/layout-cell}}
254 -{{/layout-section}}
257 +\\
255 255  
256 -{{layout-section ac:type="single"}}
257 -{{layout-cell}}
258 -----
259 +//**Set run parameters and go 🏃💥**//
259 259  
260 -Click "Start/Run" icon in kamzik.
261 +1. Set your desired No. of frames
262 +1. Match exposure time with chopper setting or set desired exposure time
263 +1. Check trigger setting and match to chopper setting
264 +1. To start the run, click "<Call>" next to //start_run (exposure    )//
265 +{{/layout-cell}}
261 261  
262 -[[image:attach:kamzik_run-tile.jpg||height="400"]]
263 -
264 -----
267 +{{layout-cell}}
268 +[[image:attach:Kamzik_run(2).PNG||height="400"]]
265 265  {{/layout-cell}}
266 266  {{/layout-section}}
267 267  
268 268  {{layout-section ac:type="two_equal"}}
269 269  {{layout-cell}}
270 -**Set run parameters and go 🏃💥**
271 -
272 -1. Set your desired No. of frames
273 -1. Match exposure time with chopper setting
274 -1. Check trigger setting and match to chopper setting
275 -1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
276 -1. To start the run, click "<Call>" next to //exposure time//
277 -
278 -\\
279 -
280 -\\
281 -
282 -**After data collection/Before entering the exp. hutch:**
283 -
284 -1. Go to "Safety" in kamzik.
285 -1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
286 -11. Drive the collimator out
287 -11. Drive the detector back to 1500 mm
288 -1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
289 -
290 -[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]]
291 -{{/layout-cell}}
292 -
293 -{{layout-cell}}
294 -(% class="wrapped relative-table" style="width: 100.0%;" %)
274 +(% class="relative-table" %)
295 295  |=(((
296 296  Parameter
297 297  )))|=(((
... ... @@ -315,7 +315,7 @@
315 315  
316 316  \\
317 317  )))|(% colspan="1" %)(((
318 -chopper at 133 Hz → 3.7 ms
298 +chopper at 133 Hz → 7.7 ms (opening time will be 3.7)
319 319  
320 320  chopper at 66 Hz   → 7.7 ms
321 321  
... ... @@ -344,16 +344,19 @@
344 344  )))|(% colspan="1" %)(((
345 345  200.0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
346 346  )))
347 -|(% colspan="1" %)(((
348 -Diode voltage
349 -)))|(% colspan="1" %)(((
350 -read out of diode
351 -)))|(% colspan="1" %)(((
352 -**~~ 0 !!! higher values indicate missaligned beam stop!!! 
353 -Call local contact
354 -**
355 -)))
356 356  {{/layout-cell}}
328 +
329 +{{layout-cell}}
330 +**After data collection/Before entering the exp. hutch:**
331 +
332 +1. Go to "Safety" in kamzik.
333 +1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
334 +11. Drive the collimator out
335 +11. Drive the detector back to 1500 mm
336 +1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
337 +
338 +[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]]
339 +{{/layout-cell}}
357 357  {{/layout-section}}
358 358  
359 359  {{layout-section ac:type="single"}}
Confluence.Code.ConfluencePageClass[0]
Id
... ... @@ -1,1 +1,1 @@
1 -250178644
1 +254729370
URL
... ... @@ -1,1 +1,1 @@
1 -https://confluence.desy.de/spaces/11/pages/250178644/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/254729370/14. Serial Crystallography: CFEL TapeDrive