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Last modified by chatzies on 2025-11-27 11:35
From version 58.1
edited by ahenkel
on 2022-04-04 20:02
Change comment: There is no comment for this version
To version 76.1
edited by ahenkel
on 2022-11-02 16:25
Change comment: There is no comment for this version

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17 17  
18 18  Preparation:
19 19  
20 -* filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
20 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
21 21  
22 22  [[image:attach:20211201_095534.jpg||height="250"]]
23 23  
... ... @@ -57,7 +57,7 @@
57 57  
58 58  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
59 59  
60 -* Click on the 'main tape' icon
60 +* Click the 'main tape' icon
61 61  * Change the velocity by typing anything from **0.3-3.0 mm/s**
62 62  * Common velocity is 1.0-1.5 mm/s
63 63  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
... ... @@ -66,7 +66,7 @@
66 66  
67 67  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
68 68  
69 -* Click an the 'nozzle' icon.
69 +* Click on the 'nozzle' icon.
70 70  * (((
71 71  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
72 72  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
... ... @@ -214,12 +214,15 @@
214 214  * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
215 215  * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps
216 216  ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", .....
217 -* (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms//
217 +* (% style="color: rgb(0,0,0);" %)//usually 130 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 130 Hz
218 +//
218 218  {{/layout-cell}}
219 219  {{/layout-section}}
220 220  
221 -{{layout-section ac:type="two_right_sidebar"}}
222 +{{layout-section ac:type="single"}}
222 222  {{layout-cell}}
224 +----
225 +
223 223  == {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** ==
224 224  
225 225  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
... ... @@ -226,7 +226,7 @@
226 226  
227 227  (% class="code" %)
228 228  (((
229 - xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
232 + xfreerdp +clipboard /size:1920x1080 /u:cfelusr /v:cfeld-tapetop
230 230   password: written on control laptop (changes every 6 months)
231 231  )))
232 232  
... ... @@ -233,65 +233,50 @@
233 233   //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
234 234  
235 235  \\
239 +{{/layout-cell}}
240 +{{/layout-section}}
236 236  
242 +{{layout-section ac:type="two_left_sidebar"}}
243 +{{layout-cell}}
237 237  **Check list:**
238 238  
239 239  (% style="color: rgb(255,0,0);" %)//always after entering the hutch!//
240 240  
241 -(% style="color: rgb(0,0,0);" %)Click "Safety" icon in kamzik.
248 +(% style="color: rgb(0,0,0);" %)Click "Run" icon in kamzik.
242 242  
243 -* Put collimator in
244 -** Click "<Call>" next to //collimator_in//
250 +* Put detector distance 200 in //prepare_for_measurement (      )
251 +//
252 +* Click "<Call>" next to //prepare_for_measurement
253 +//This will:\\
254 +** drive the collimator in //IN// position
255 +** drive detector to 200 mm
245 245  
246 246  \\
247 247  
248 -* Open beam shutter BS1 in //CrystalControl//
249 -{{/layout-cell}}
259 +* Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed //
250 250  
251 -{{layout-cell}}
252 -[[image:attach:kamzik_safety.PNG||height="250"]]
253 -{{/layout-cell}}
254 -{{/layout-section}}
261 +\\
255 255  
256 -{{layout-section ac:type="single"}}
257 -{{layout-cell}}
258 -----
263 +//**Set run parameters and go 🏃💥**//
259 259  
260 -Click "Start/Run" icon in kamzik.
261 -
262 -[[image:attach:kamzik_run-tile.jpg||height="400"]]
263 -
264 -----
265 -{{/layout-cell}}
266 -{{/layout-section}}
267 -
268 -{{layout-section ac:type="two_equal"}}
269 -{{layout-cell}}
270 -**Set run parameters and go 🏃💥**
271 -
272 272  1. Set your desired No. of frames
273 -1. Match exposure time with chopper setting
266 +1. Enter the protein ID of your crystal sample
267 +1. Match exposure time with chopper setting or set desired exposure time
274 274  1. Check trigger setting and match to chopper setting
275 -1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
276 -1. To start the run, click "<Call>" next to //exposure time//
269 +no chopper → external triffer //off
270 +// chopper → external trigger// on
271 +//
272 +1. To start the run, click "<Call>" next to //start_run (exposure    )//
273 +{{/layout-cell}}
277 277  
278 -\\
279 -
280 -\\
281 -
282 -**After data collection/Before entering the exp. hutch:**
283 -
284 -1. Go to "Safety" in kamzik.
285 -1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
286 -11. Drive the collimator out
287 -11. Drive the detector back to 1500 mm
288 -1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
289 -
290 -[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]]
275 +{{layout-cell}}
276 +[[image:attach:Kamzik_run(2).PNG]]
291 291  {{/layout-cell}}
278 +{{/layout-section}}
292 292  
280 +{{layout-section ac:type="single"}}
293 293  {{layout-cell}}
294 -(% class="wrapped relative-table" style="width: 100.0%;" %)
282 +(% class="relative-table wrapped" style="width: 55.9569%;" %)
295 295  |=(((
296 296  Parameter
297 297  )))|=(((
... ... @@ -302,7 +302,7 @@
302 302  |(((
303 303  Images number
304 304  )))|(((
305 -Set number of frames you want to collect
293 +number of frames you want to collect
306 306  )))|(% colspan="1" %)(((
307 307  usually 100k - 200k, but adjust according to hitrate
308 308  
... ... @@ -315,10 +315,7 @@
315 315  
316 316  \\
317 317  )))|(% colspan="1" %)(((
318 -chopper at 133 Hz → 3.7 ms
319 -
320 -chopper at 66 Hz   → 7.7 ms
321 -
306 +with chopper         → opening time displayed in chopper GUI
322 322  no chopper            → 7.7 ms
323 323  
324 324  ----
... ... @@ -338,24 +338,45 @@
338 338  \\
339 339  )))
340 340  |(% colspan="1" %)(((
341 -move_detector (distance)
326 +protein ID
342 342  )))|(% colspan="1" %)(((
343 -Set detector distance
328 +protein ID of crystal sample (see AMARCORD)
344 344  )))|(% colspan="1" %)(((
345 -200.0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
330 +\\
346 346  )))
347 -|(% colspan="1" %)(((
348 -Diode voltage
349 -)))|(% colspan="1" %)(((
350 -read out of diode
351 -)))|(% colspan="1" %)(((
352 -**~~ 0 !!! higher values indicate missaligned beam stop!!! 
353 -Call local contact
354 -**
355 -)))
332 +
333 +----
356 356  {{/layout-cell}}
357 357  {{/layout-section}}
358 358  
337 +{{layout-section ac:type="two_equal"}}
338 +{{layout-cell}}
339 +**After data collection/Before entering the exp. hutch:**
340 +
341 +1. Go to "Safety" in kamzik.
342 +1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
343 +11. Drive the collimator out
344 +11. Drive the detector back to 1500 mm
345 +1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
346 +
347 +[[image:attach:Screenshot from 2022-04-04 19-36-29.png]]
348 +{{/layout-cell}}
349 +
350 +{{layout-cell}}
351 +\\
352 +
353 +\\
354 +
355 +\\
356 +
357 +\\
358 +
359 +\\
360 +
361 +[[image:attach:safety_kamzik.PNG]]
362 +{{/layout-cell}}
363 +{{/layout-section}}
364 +
359 359  {{layout-section ac:type="single"}}
360 360  {{layout-cell}}
361 361  ----
... ... @@ -416,6 +416,7 @@
416 416  )))
417 417  )))
418 418  )))
425 +* After the Simplon server is back up, close all kamzik windows and restart (first server, then client)
419 419  )))
420 420  {{/layout-cell}}
421 421  {{/layout-section}}
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Id
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1 -250178654
1 +235031805
URL
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1 -https://confluence.desy.de/spaces/11/pages/250178654/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/235031805/14. Serial Crystallography: CFEL TapeDrive