Changes for page CFEL TapeDrive: Serial Crystallography
Last modified by chatzies on 2025-11-27 11:35
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... ... @@ -214,7 +214,7 @@ 214 214 * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes// 215 215 * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps 216 216 ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", ..... 217 -* (% style="color: rgb(0,0,0);" %)//usually 13 3Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 133Hz217 +* (% style="color: rgb(0,0,0);" %)//usually 130 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 130 Hz 218 218 // 219 219 {{/layout-cell}} 220 220 {{/layout-section}} ... ... @@ -221,6 +221,8 @@ 221 221 222 222 {{layout-section ac:type="single"}} 223 223 {{layout-cell}} 224 +---- 225 + 224 224 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** == 225 225 226 226 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command: ... ... @@ -227,7 +227,7 @@ 227 227 228 228 (% class="code" %) 229 229 ((( 230 - xfreerdp +clipboard /size:1920x1080 /u: Cfelusr /v:cfeld-pcx22611232 + xfreerdp +clipboard /size:1920x1080 /u:cfelusr /v:cfeld-tapetop 231 231 password: written on control laptop (changes every 6 months) 232 232 ))) 233 233 ... ... @@ -243,60 +243,41 @@ 243 243 244 244 (% style="color: rgb(255,0,0);" %)//always after entering the hutch!// 245 245 246 -(% style="color: rgb(0,0,0);" %)Click " Safety" icon in kamzik.248 +(% style="color: rgb(0,0,0);" %)Click "Run" icon in kamzik. 247 247 248 -* Put collimator in 249 -** Click "<Call>" next to //collimator_in// 250 +* Put detector distance 200 in //prepare_for_measurement ( ) 251 +// 252 +* Click "<Call>" next to //prepare_for_measurement 253 +//This will:\\ 254 +** drive the collimator in //IN// position 255 +** drive detector to 200 mm 250 250 251 251 \\ 252 252 253 253 * Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed // 254 -{{/layout-cell}} 255 255 256 -{{layout-cell}} 257 -[[image:attach:kamzik_safety.PNG||height="400"]] 258 -{{/layout-cell}} 259 -{{/layout-section}} 261 +\\ 260 260 261 -{{layout-section ac:type="single"}} 262 -{{layout-cell}} 263 ----- 263 +//**Set run parameters and go 🏃💥**// 264 264 265 -Click "Start/Run" icon in kamzik. 266 - 267 -[[image:attach:kamzik_run-tile.jpg||height="400"]] 268 - 269 ----- 270 -{{/layout-cell}} 271 -{{/layout-section}} 272 - 273 -{{layout-section ac:type="two_equal"}} 274 -{{layout-cell}} 275 -**Set run parameters and go 🏃💥** 276 - 277 277 1. Set your desired No. of frames 278 -1. Match exposure time with chopper setting 266 +1. Enter the protein ID of your crystal sample 267 +1. Match exposure time with chopper setting or set desired exposure time 279 279 1. Check trigger setting and match to chopper setting 280 -1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it. 281 -1. To start the run, click "<Call>" next to //exposure time// 269 +no chopper → external triffer //off 270 +// chopper → external trigger// on 271 +// 272 +1. To start the run, click "<Call>" next to //start_run (exposure )// 273 +{{/layout-cell}} 282 282 283 -\\ 284 - 285 -\\ 286 - 287 -**After data collection/Before entering the exp. hutch:** 288 - 289 -1. Go to "Safety" in kamzik. 290 -1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically: 291 -11. Drive the collimator out 292 -11. Drive the detector back to 1500 mm 293 -1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left). 294 - 295 -[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]] 275 +{{layout-cell}} 276 +[[image:attach:Kamzik_run(2).PNG]] 296 296 {{/layout-cell}} 278 +{{/layout-section}} 297 297 280 +{{layout-section ac:type="single"}} 298 298 {{layout-cell}} 299 -(% class=" wrapped relative-table" style="width:100.0%;" %)282 +(% class="relative-table wrapped" style="width: 55.9569%;" %) 300 300 |=((( 301 301 Parameter 302 302 )))|=((( ... ... @@ -307,7 +307,7 @@ 307 307 |((( 308 308 Images number 309 309 )))|((( 310 - Setnumber of frames you want to collect293 +number of frames you want to collect 311 311 )))|(% colspan="1" %)((( 312 312 usually 100k - 200k, but adjust according to hitrate 313 313 ... ... @@ -320,10 +320,7 @@ 320 320 321 321 \\ 322 322 )))|(% colspan="1" %)((( 323 -chopper at 133 Hz → 7.7 ms (opening time will be 3.7) 324 - 325 -chopper at 66 Hz → 7.7 ms 326 - 306 +with chopper → opening time displayed in chopper GUI 327 327 no chopper → 7.7 ms 328 328 329 329 ---- ... ... @@ -343,15 +343,45 @@ 343 343 \\ 344 344 ))) 345 345 |(% colspan="1" %)((( 346 - move_detector (distance)326 +protein ID 347 347 )))|(% colspan="1" %)((( 348 - Setdetectordistance328 +protein ID of crystal sample (see AMARCORD) 349 349 )))|(% colspan="1" %)((( 350 - 200.0 mm (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"330 +\\ 351 351 ))) 332 + 333 +---- 352 352 {{/layout-cell}} 353 353 {{/layout-section}} 354 354 337 +{{layout-section ac:type="two_equal"}} 338 +{{layout-cell}} 339 +**After data collection/Before entering the exp. hutch:** 340 + 341 +1. Go to "Safety" in kamzik. 342 +1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically: 343 +11. Drive the collimator out 344 +11. Drive the detector back to 1500 mm 345 +1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left). 346 + 347 +[[image:attach:Screenshot from 2022-04-04 19-36-29.png]] 348 +{{/layout-cell}} 349 + 350 +{{layout-cell}} 351 +\\ 352 + 353 +\\ 354 + 355 +\\ 356 + 357 +\\ 358 + 359 +\\ 360 + 361 +[[image:attach:safety_kamzik.PNG]] 362 +{{/layout-cell}} 363 +{{/layout-section}} 364 + 355 355 {{layout-section ac:type="single"}} 356 356 {{layout-cell}} 357 357 ---- ... ... @@ -412,6 +412,7 @@ 412 412 ))) 413 413 ))) 414 414 ))) 425 +* After the Simplon server is back up, close all kamzik windows and restart (first server, then client) 415 415 ))) 416 416 {{/layout-cell}} 417 417 {{/layout-section}}
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... ... @@ -1,1 +1,1 @@ 1 -https://confluence.desy.de/spaces/11/pages/25 4728507/14. Serial Crystallography: CFEL TapeDrive1 +https://confluence.desy.de/spaces/11/pages/235031805/14. Serial Crystallography: CFEL TapeDrive