Changes for page CFEL TapeDrive: Serial Crystallography
Last modified by chatzies on 2025-11-27 11:35
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... ... @@ -214,7 +214,7 @@ 214 214 * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes// 215 215 * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps 216 216 ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", ..... 217 -* (% style="color: rgb(0,0,0);" %)//usually 13 3Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 133Hz217 +* (% style="color: rgb(0,0,0);" %)//usually 130 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 130 Hz 218 218 // 219 219 {{/layout-cell}} 220 220 {{/layout-section}} ... ... @@ -221,6 +221,8 @@ 221 221 222 222 {{layout-section ac:type="single"}} 223 223 {{layout-cell}} 224 +---- 225 + 224 224 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** == 225 225 226 226 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command: ... ... @@ -227,7 +227,7 @@ 227 227 228 228 (% class="code" %) 229 229 ((( 230 - xfreerdp +clipboard /size:1920x1080 /u: Cfelusr /v:cfeld-pcx22611232 + xfreerdp +clipboard /size:1920x1080 /u:cfelusr /v:cfeld-tapetop 231 231 password: written on control laptop (changes every 6 months) 232 232 ))) 233 233 ... ... @@ -245,58 +245,39 @@ 245 245 246 246 (% style="color: rgb(0,0,0);" %)Click "Run" icon in kamzik. 247 247 248 -* Put collimator in 249 -** Put detector distance 200 in //prepare_for_measurement ( ) 250 +* Put detector distance 200 in //prepare_for_measurement ( ) 250 250 // 251 -** Click "<Call>" next to //prepare_for_measurement// 252 +* Click "<Call>" next to //prepare_for_measurement 253 +//This will:\\ 254 +** drive the collimator in //IN// position 255 +** drive detector to 200 mm 252 252 253 253 \\ 254 254 255 255 * Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed // 256 -{{/layout-cell}} 257 257 258 -{{layout-cell}} 259 -[[image:attach:kamzik_safety.PNG||height="400"]] 260 -{{/layout-cell}} 261 -{{/layout-section}} 261 +\\ 262 262 263 -{{layout-section ac:type="single"}} 264 -{{layout-cell}} 265 ----- 263 +//**Set run parameters and go 🏃💥**// 266 266 267 -[[image:attach:kamzik_run-tile.jpg||height="400"]] 268 - 269 ----- 270 -{{/layout-cell}} 271 -{{/layout-section}} 272 - 273 -{{layout-section ac:type="two_equal"}} 274 -{{layout-cell}} 275 -**Set run parameters and go 🏃💥** 276 - 277 277 1. Set your desired No. of frames 266 +1. Enter the protein ID of your crystal sample 278 278 1. Match exposure time with chopper setting or set desired exposure time 279 279 1. Check trigger setting and match to chopper setting 280 -1. To start the run, click "<Call>" next to //start_run (exposure ) 269 +no chopper → external triffer //off 270 +// chopper → external trigger// on 281 281 // 272 +1. To start the run, click "<Call>" next to //start_run (exposure )// 273 +{{/layout-cell}} 282 282 283 -\\ 284 - 285 -\\ 286 - 287 -**After data collection/Before entering the exp. hutch:** 288 - 289 -1. Go to "Safety" in kamzik. 290 -1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically: 291 -11. Drive the collimator out 292 -11. Drive the detector back to 1500 mm 293 -1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left). 294 - 295 -[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]] 275 +{{layout-cell}} 276 +[[image:attach:Kamzik_run(2).PNG]] 296 296 {{/layout-cell}} 278 +{{/layout-section}} 297 297 280 +{{layout-section ac:type="single"}} 298 298 {{layout-cell}} 299 -(% class=" wrapped relative-table" style="width:100.0%;" %)282 +(% class="relative-table wrapped" style="width: 55.9569%;" %) 300 300 |=((( 301 301 Parameter 302 302 )))|=((( ... ... @@ -307,7 +307,7 @@ 307 307 |((( 308 308 Images number 309 309 )))|((( 310 - Setnumber of frames you want to collect293 +number of frames you want to collect 311 311 )))|(% colspan="1" %)((( 312 312 usually 100k - 200k, but adjust according to hitrate 313 313 ... ... @@ -320,10 +320,7 @@ 320 320 321 321 \\ 322 322 )))|(% colspan="1" %)((( 323 -chopper at 133 Hz → 7.7 ms (opening time will be 3.7) 324 - 325 -chopper at 66 Hz → 7.7 ms 326 - 306 +with chopper → opening time displayed in chopper GUI 327 327 no chopper → 7.7 ms 328 328 329 329 ---- ... ... @@ -343,15 +343,45 @@ 343 343 \\ 344 344 ))) 345 345 |(% colspan="1" %)((( 346 - move_detector (distance)326 +protein ID 347 347 )))|(% colspan="1" %)((( 348 - Setdetectordistance328 +protein ID of crystal sample (see AMARCORD) 349 349 )))|(% colspan="1" %)((( 350 - 200.0 mm (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"330 +\\ 351 351 ))) 332 + 333 +---- 352 352 {{/layout-cell}} 353 353 {{/layout-section}} 354 354 337 +{{layout-section ac:type="two_equal"}} 338 +{{layout-cell}} 339 +**After data collection/Before entering the exp. hutch:** 340 + 341 +1. Go to "Safety" in kamzik. 342 +1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically: 343 +11. Drive the collimator out 344 +11. Drive the detector back to 1500 mm 345 +1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left). 346 + 347 +[[image:attach:Screenshot from 2022-04-04 19-36-29.png]] 348 +{{/layout-cell}} 349 + 350 +{{layout-cell}} 351 +\\ 352 + 353 +\\ 354 + 355 +\\ 356 + 357 +\\ 358 + 359 +\\ 360 + 361 +[[image:attach:safety_kamzik.PNG]] 362 +{{/layout-cell}} 363 +{{/layout-section}} 364 + 355 355 {{layout-section ac:type="single"}} 356 356 {{layout-cell}} 357 357 ---- ... ... @@ -412,6 +412,7 @@ 412 412 ))) 413 413 ))) 414 414 ))) 425 +* After the Simplon server is back up, close all kamzik windows and restart (first server, then client) 415 415 ))) 416 416 {{/layout-cell}} 417 417 {{/layout-section}}
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... ... @@ -1,1 +1,1 @@ 1 -https://confluence.desy.de/spaces/11/pages/25 4729368/14. Serial Crystallography: CFEL TapeDrive1 +https://confluence.desy.de/spaces/11/pages/235031805/14. Serial Crystallography: CFEL TapeDrive