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Last modified by chatzies on 2025-11-27 11:35
From version 76.1
edited by ahenkel
on 2022-11-02 16:25
Change comment: There is no comment for this version
To version 57.1
edited by ahenkel
on 2022-04-04 20:01
Change comment: There is no comment for this version

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17 17  
18 18  Preparation:
19 19  
20 -* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
20 +* filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
21 21  
22 22  [[image:attach:20211201_095534.jpg||height="250"]]
23 23  
... ... @@ -57,7 +57,7 @@
57 57  
58 58  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
59 59  
60 -* Click the 'main tape' icon
60 +* Click on the 'main tape' icon
61 61  * Change the velocity by typing anything from **0.3-3.0 mm/s**
62 62  * Common velocity is 1.0-1.5 mm/s
63 63  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
... ... @@ -66,7 +66,7 @@
66 66  
67 67  ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
68 68  
69 -* Click on the 'nozzle' icon.
69 +* Click an the 'nozzle' icon.
70 70  * (((
71 71  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
72 72  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
... ... @@ -214,15 +214,12 @@
214 214  * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
215 215  * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps
216 216  ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", .....
217 -* (% style="color: rgb(0,0,0);" %)//usually 130 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 130 Hz
218 -//
217 +* (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms//
219 219  {{/layout-cell}}
220 220  {{/layout-section}}
221 221  
222 -{{layout-section ac:type="single"}}
221 +{{layout-section ac:type="two_equal"}}
223 223  {{layout-cell}}
224 -----
225 -
226 226  == {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** ==
227 227  
228 228  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
... ... @@ -229,7 +229,7 @@
229 229  
230 230  (% class="code" %)
231 231  (((
232 - xfreerdp +clipboard /size:1920x1080 /u:cfelusr /v:cfeld-tapetop
229 + xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
233 233   password: written on control laptop (changes every 6 months)
234 234  )))
235 235  
... ... @@ -236,50 +236,65 @@
236 236   //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
237 237  
238 238  \\
239 -{{/layout-cell}}
240 -{{/layout-section}}
241 241  
242 -{{layout-section ac:type="two_left_sidebar"}}
243 -{{layout-cell}}
244 244  **Check list:**
245 245  
246 246  (% style="color: rgb(255,0,0);" %)//always after entering the hutch!//
247 247  
248 -(% style="color: rgb(0,0,0);" %)Click "Run" icon in kamzik.
241 +(% style="color: rgb(0,0,0);" %)Click "Safety" icon in kamzik.
249 249  
250 -* Put detector distance 200 in //prepare_for_measurement (      )
251 -//
252 -* Click "<Call>" next to //prepare_for_measurement
253 -//This will:\\
254 -** drive the collimator in //IN// position
255 -** drive detector to 200 mm
243 +* Put collimator in
244 +** Click "<Call>" next to //collimator_in//
256 256  
257 257  \\
258 258  
259 -* Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed //
248 +* Open beam shutter BS1 in //CrystalControl//
249 +{{/layout-cell}}
260 260  
261 -\\
251 +{{layout-cell}}
252 +[[image:attach:kamzik_safety.PNG||height="250"]]
253 +{{/layout-cell}}
254 +{{/layout-section}}
262 262  
263 -//**Set run parameters and go 🏃💥**//
256 +{{layout-section ac:type="single"}}
257 +{{layout-cell}}
258 +----
264 264  
265 -1. Set your desired No. of frames
266 -1. Enter the protein ID of your crystal sample
267 -1. Match exposure time with chopper setting or set desired exposure time
268 -1. Check trigger setting and match to chopper setting
269 -no chopper → external triffer //off
270 -// chopper → external trigger// on
271 -//
272 -1. To start the run, click "<Call>" next to //start_run (exposure    )//
260 +Click "Start/Run" icon in kamzik.
261 +
262 +[[image:attach:kamzik_run-tile.jpg||height="400"]]
263 +
264 +----
273 273  {{/layout-cell}}
266 +{{/layout-section}}
274 274  
268 +{{layout-section ac:type="two_equal"}}
275 275  {{layout-cell}}
276 -[[image:attach:Kamzik_run(2).PNG]]
270 +**Set run parameters and go 🏃💥**
271 +
272 +1. Set your desired No. of frames
273 +1. Match exposure time with chopper setting
274 +1. Check trigger setting and match to chopper setting
275 +1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
276 +1. To start the run, click "<Call>" next to //exposure time//
277 +
278 +\\
279 +
280 +\\
281 +
282 +**After data collection/Before entering the exp. hutch:**
283 +
284 +1. Go to "Safety" in kamzik.
285 +1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
286 +11. Drive the collimator out
287 +11. Drive the detector back to 1500 mm
288 +1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
289 +
290 +[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]]
277 277  {{/layout-cell}}
278 -{{/layout-section}}
279 279  
280 -{{layout-section ac:type="single"}}
281 281  {{layout-cell}}
282 -(% class="relative-table wrapped" style="width: 55.9569%;" %)
294 +(% class="wrapped relative-table" style="width: 100.0%;" %)
283 283  |=(((
284 284  Parameter
285 285  )))|=(((
... ... @@ -290,7 +290,7 @@
290 290  |(((
291 291  Images number
292 292  )))|(((
293 -number of frames you want to collect
305 +Set number of frames you want to collect
294 294  )))|(% colspan="1" %)(((
295 295  usually 100k - 200k, but adjust according to hitrate
296 296  
... ... @@ -303,7 +303,10 @@
303 303  
304 304  \\
305 305  )))|(% colspan="1" %)(((
306 -with chopper         → opening time displayed in chopper GUI
318 +chopper at 133 Hz → 3.7 ms
319 +
320 +chopper at 66 Hz   → 7.7 ms
321 +
307 307  no chopper            → 7.7 ms
308 308  
309 309  ----
... ... @@ -323,45 +323,24 @@
323 323  \\
324 324  )))
325 325  |(% colspan="1" %)(((
326 -protein ID
341 +move_detector (distance)
327 327  )))|(% colspan="1" %)(((
328 -protein ID of crystal sample (see AMARCORD)
343 +Set detector distance
329 329  )))|(% colspan="1" %)(((
330 -\\
345 +200.0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
331 331  )))
332 -
333 -----
347 +|(% colspan="1" %)(((
348 +Diode voltage
349 +)))|(% colspan="1" %)(((
350 +read out of diode
351 +)))|(% colspan="1" %)(((
352 +**~~ 0 !!! higher values indicate missaligned beam stop!!! 
353 +Call local contact
354 +**
355 +)))
334 334  {{/layout-cell}}
335 335  {{/layout-section}}
336 336  
337 -{{layout-section ac:type="two_equal"}}
338 -{{layout-cell}}
339 -**After data collection/Before entering the exp. hutch:**
340 -
341 -1. Go to "Safety" in kamzik.
342 -1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
343 -11. Drive the collimator out
344 -11. Drive the detector back to 1500 mm
345 -1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
346 -
347 -[[image:attach:Screenshot from 2022-04-04 19-36-29.png]]
348 -{{/layout-cell}}
349 -
350 -{{layout-cell}}
351 -\\
352 -
353 -\\
354 -
355 -\\
356 -
357 -\\
358 -
359 -\\
360 -
361 -[[image:attach:safety_kamzik.PNG]]
362 -{{/layout-cell}}
363 -{{/layout-section}}
364 -
365 365  {{layout-section ac:type="single"}}
366 366  {{layout-cell}}
367 367  ----
... ... @@ -422,7 +422,6 @@
422 422  )))
423 423  )))
424 424  )))
425 -* After the Simplon server is back up, close all kamzik windows and restart (first server, then client)
426 426  )))
427 427  {{/layout-cell}}
428 428  {{/layout-section}}
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1 -235031805
1 +250178651
URL
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1 -https://confluence.desy.de/spaces/11/pages/235031805/14. Serial Crystallography: CFEL TapeDrive
1 +https://confluence.desy.de/spaces/11/pages/250178651/14. Serial Crystallography: CFEL TapeDrive