Changes for page CFEL TapeDrive: Serial Crystallography
Last modified by chatzies on 2025-11-27 11:35
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... ... @@ -214,7 +214,7 @@ 214 214 * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes// 215 215 * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps 216 216 ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", ..... 217 -* (% style="color: rgb(0,0,0);" %)//usually 13 0Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 130Hz217 +* (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 133 Hz 218 218 // 219 219 {{/layout-cell}} 220 220 {{/layout-section}} ... ... @@ -221,8 +221,6 @@ 221 221 222 222 {{layout-section ac:type="single"}} 223 223 {{layout-cell}} 224 ----- 225 - 226 226 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** == 227 227 228 228 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command: ... ... @@ -229,7 +229,7 @@ 229 229 230 230 (% class="code" %) 231 231 ((( 232 - xfreerdp +clipboard /size:1920x1080 /u: cfelusr /v:cfeld-tapetop230 + xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611 233 233 password: written on control laptop (changes every 6 months) 234 234 ))) 235 235 ... ... @@ -247,39 +247,58 @@ 247 247 248 248 (% style="color: rgb(0,0,0);" %)Click "Run" icon in kamzik. 249 249 250 -* Put detector distance 200 in //prepare_for_measurement ( ) 248 +* Put collimator in 249 +** Put detector distance 200 in //prepare_for_measurement ( ) 251 251 // 252 -* Click "<Call>" next to //prepare_for_measurement 253 -//This will:\\ 254 -** drive the collimator in //IN// position 255 -** drive detector to 200 mm 251 +** Click "<Call>" next to //prepare_for_measurement// 256 256 257 257 \\ 258 258 259 259 * Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed // 256 +{{/layout-cell}} 260 260 261 -\\ 258 +{{layout-cell}} 259 +[[image:attach:kamzik_safety.PNG||height="400"]] 260 +{{/layout-cell}} 261 +{{/layout-section}} 262 262 263 -//**Set run parameters and go 🏃💥**// 263 +{{layout-section ac:type="single"}} 264 +{{layout-cell}} 265 +---- 264 264 267 +[[image:attach:kamzik_run-tile.jpg||height="400"]] 268 + 269 +---- 270 +{{/layout-cell}} 271 +{{/layout-section}} 272 + 273 +{{layout-section ac:type="two_equal"}} 274 +{{layout-cell}} 275 +**Set run parameters and go 🏃💥** 276 + 265 265 1. Set your desired No. of frames 266 -1. Enter the protein ID of your crystal sample 267 267 1. Match exposure time with chopper setting or set desired exposure time 268 268 1. Check trigger setting and match to chopper setting 269 -no chopper → external triffer //off 270 -// chopper → external trigger// on 280 +1. To start the run, click "<Call>" next to //start_run (exposure ) 271 271 // 272 -1. To start the run, click "<Call>" next to //start_run (exposure )// 273 -{{/layout-cell}} 274 274 275 -{{layout-cell}} 276 -[[image:attach:Kamzik_run(2).PNG]] 283 +\\ 284 + 285 +\\ 286 + 287 +**After data collection/Before entering the exp. hutch:** 288 + 289 +1. Go to "Safety" in kamzik. 290 +1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically: 291 +11. Drive the collimator out 292 +11. Drive the detector back to 1500 mm 293 +1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left). 294 + 295 +[[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]] 277 277 {{/layout-cell}} 278 -{{/layout-section}} 279 279 280 -{{layout-section ac:type="single"}} 281 281 {{layout-cell}} 282 -(% class="relative-table wrapped" style="width:55.9569%;" %)299 +(% class="wrapped relative-table" style="width: 100.0%;" %) 283 283 |=((( 284 284 Parameter 285 285 )))|=((( ... ... @@ -290,7 +290,7 @@ 290 290 |((( 291 291 Images number 292 292 )))|((( 293 -number of frames you want to collect 310 +Set number of frames you want to collect 294 294 )))|(% colspan="1" %)((( 295 295 usually 100k - 200k, but adjust according to hitrate 296 296 ... ... @@ -303,7 +303,10 @@ 303 303 304 304 \\ 305 305 )))|(% colspan="1" %)((( 306 -with chopper → opening time displayed in chopper GUI 323 +chopper at 133 Hz → 7.7 ms (opening time will be 3.7) 324 + 325 +chopper at 66 Hz → 7.7 ms 326 + 307 307 no chopper → 7.7 ms 308 308 309 309 ---- ... ... @@ -323,45 +323,15 @@ 323 323 \\ 324 324 ))) 325 325 |(% colspan="1" %)((( 326 - proteinID346 +move_detector (distance) 327 327 )))|(% colspan="1" %)((( 328 - proteinID ofcrystal sample(see AMARCORD)348 +Set detector distance 329 329 )))|(% colspan="1" %)((( 330 - \\350 +200.0 mm (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>" 331 331 ))) 332 - 333 ----- 334 334 {{/layout-cell}} 335 335 {{/layout-section}} 336 336 337 -{{layout-section ac:type="two_equal"}} 338 -{{layout-cell}} 339 -**After data collection/Before entering the exp. hutch:** 340 - 341 -1. Go to "Safety" in kamzik. 342 -1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically: 343 -11. Drive the collimator out 344 -11. Drive the detector back to 1500 mm 345 -1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left). 346 - 347 -[[image:attach:Screenshot from 2022-04-04 19-36-29.png]] 348 -{{/layout-cell}} 349 - 350 -{{layout-cell}} 351 -\\ 352 - 353 -\\ 354 - 355 -\\ 356 - 357 -\\ 358 - 359 -\\ 360 - 361 -[[image:attach:safety_kamzik.PNG]] 362 -{{/layout-cell}} 363 -{{/layout-section}} 364 - 365 365 {{layout-section ac:type="single"}} 366 366 {{layout-cell}} 367 367 ---- ... ... @@ -422,7 +422,6 @@ 422 422 ))) 423 423 ))) 424 424 ))) 425 -* After the Simplon server is back up, close all kamzik windows and restart (first server, then client) 426 426 ))) 427 427 {{/layout-cell}} 428 428 {{/layout-section}}
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... ... @@ -1,1 +1,1 @@ 1 -https://confluence.desy.de/spaces/11/pages/2 35031805/14. Serial Crystallography: CFEL TapeDrive1 +https://confluence.desy.de/spaces/11/pages/254729368/14. Serial Crystallography: CFEL TapeDrive