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Last modified by chatzies on 2025-11-27 11:35
From version 79.4
edited by tabermah
on 2024-12-09 10:50
Change comment: allow edit right for XWiki.ahenkel
To version 83.1
edited by tabermah
on 2024-12-10 15:24
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... ... @@ -4,18 +4,18 @@
4 4  [[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
5 5  
6 6  
7 -{{toc minLevel="2"/}}
7 +{{toc/}}
8 8  
9 9  
10 10  ----
11 11  
12 -== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}**8.1 Sample characteristics and preparation** ==
12 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =
13 13  
14 -suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
14 +* Suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
15 15  
16 16  Preparation:
17 17  
18 -* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
18 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into an Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
... ... @@ -22,11 +22,8 @@
22 22  
23 23  ----
24 24  
25 -== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}**8.2 Connecting sample to ElveFlow, sample exchange** ==
25 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
26 26  
27 -*
28 -** ==
29 -
30 30  After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
31 31  
32 32  Be aware to which channel and nozzle line your sample is connected.
... ... @@ -44,18 +44,16 @@
44 44  
45 45  ----
46 46  
47 -== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}**8.3 TapeDrive operation** ==
44 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3. TapeDrive operation =
48 48  
49 -*
50 -** ==
46 +​​​​​​The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
51 51  
52 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
53 53  Now start the client. Same thing, a terminal will open, shortly after a small window.
54 54  
55 55  Click 'Devices' in the **client session** to open the control window.
56 56  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
57 57  
58 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
53 +== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}Tape ==
59 59  
60 60  * Click the 'main tape' icon
61 61  * Change the velocity by typing anything from **0.3-3.0 mm/s**
... ... @@ -64,7 +64,7 @@
64 64  
65 65  * [[image:attach:kamzik_tape.PNG||height="400"]]
66 66  
67 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
62 +== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle ==
68 68  
69 69  * Click on the 'nozzle' icon.
70 70  * (((
... ... @@ -120,14 +120,14 @@
120 120  )))
121 121  * [[image:attach:kamzik_nozzle.PNG||height="400"]]
122 122  
123 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
118 +== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller ==
124 124  
125 125  * The chiller circulates fluid through the gas nozzle.
126 126  
127 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ====
122 +== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}Gas nozzle ==
128 128  
129 129  
130 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ====
125 +== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}IR camera ==
131 131  
132 132  
133 133  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
... ... @@ -139,7 +139,7 @@
139 139  
140 140  ----
141 141  
142 -== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}**8.4 Tape roll exchange** ==
137 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4. Tape roll exchange =
143 143  
144 144  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
145 145  
... ... @@ -167,7 +167,7 @@
167 167  {{layout-cell}}
168 168  ----
169 169  
170 -== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}**8.5 Chopper** ==
165 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5. Chopper =
171 171  
172 172  start chopper gui from terminal:
173 173  
... ... @@ -210,7 +210,7 @@
210 210  {{layout-cell}}
211 211  ----
212 212  
213 -== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**8.6 Data collection** ==
208 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6. Data collection =
214 214  
215 215  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
216 216  
... ... @@ -343,10 +343,10 @@
343 343  {{layout-cell}}
344 344  ----
345 345  
346 -== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}**8.7 Troubleshooting** ==
341 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7. Troubleshooting =
347 347  
348 348  * (((
349 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
344 +== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==
350 350  
351 351  * Tape roll empty?
352 352  * motor connected (icon green)?
... ... @@ -353,7 +353,7 @@
353 353  )))
354 354  
355 355  * (((
356 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
351 +== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ==
357 357  
358 358  * displayed in green=connected?
359 359  * nozzle holder colliding somewhere?
... ... @@ -361,7 +361,7 @@
361 361  )))
362 362  
363 363  * (((
364 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
359 +== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ==
365 365  
366 366  * channel turned on?
367 367  * Sample empty?
... ... @@ -369,7 +369,7 @@
369 369  )))
370 370  
371 371  * (((
372 -==== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ====
367 +== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ==
373 373  
374 374  * First, restart kamzik (server+client) and see if this solves the issue
375 375  * If not, restart the Simplon server (in Astor → haspp11exp03, right click on Simplon/EH.1 and restart)