Skip to Content
Last modified by chatzies on 2025-11-27 11:35
From version 80.1
edited by tabermah
on 2024-12-10 15:20
Change comment: There is no comment for this version
To version 83.4
edited by tabermah
on 2024-12-10 15:50
Change comment: There is no comment for this version

Summary

Details

Page properties
Content
... ... @@ -9,13 +9,13 @@
9 9  
10 10  ----
11 11  
12 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1 Sample characteristics and preparation =
12 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =
13 13  
14 -suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
14 +* Suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
15 15  
16 16  Preparation:
17 17  
18 -* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
18 +* filter through 30 µm (green) or 50 µm (yellow) cell filter into an Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
... ... @@ -22,13 +22,10 @@
22 22  
23 23  ----
24 24  
25 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2 Connecting sample to ElveFlow, sample exchange =
25 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
26 26  
27 -*
28 -** ==
27 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish tube goes all the way to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
29 29  
30 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
31 -
32 32  Be aware to which channel and nozzle line your sample is connected.
33 33  
34 34   Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
... ... @@ -44,12 +44,10 @@
44 44  
45 45  ----
46 46  
47 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3 TapeDrive operation =
44 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3. TapeDrive operation =
48 48  
49 -*
50 -** ==
46 +​​​​​​The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
51 51  
52 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
53 53  Now start the client. Same thing, a terminal will open, shortly after a small window.
54 54  
55 55  Click 'Devices' in the **client session** to open the control window.
... ... @@ -62,8 +62,10 @@
62 62  * Common velocity is 1.0-1.5 mm/s
63 63  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
64 64  
65 -* [[image:attach:kamzik_tape.PNG||height="400"]]
66 66  
61 +[[image:attach:kamzik_tape.PNG||height="400"]]
62 +
63 +
67 67  == {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle ==
68 68  
69 69  * Click on the 'nozzle' icon.
... ... @@ -72,7 +72,7 @@
72 72  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
73 73  
74 74  
75 -(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
72 +(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving the nozzle, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
76 76  \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
77 77  
78 78  (% class="wrapped" %)
... ... @@ -118,8 +118,10 @@
118 118  
119 119  
120 120  )))
121 -* [[image:attach:kamzik_nozzle.PNG||height="400"]]
122 122  
119 +[[image:attach:kamzik_nozzle.PNG||height="400"]]
120 +
121 +
123 123  == {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller ==
124 124  
125 125  * The chiller circulates fluid through the gas nozzle.
... ... @@ -139,7 +139,7 @@
139 139  
140 140  ----
141 141  
142 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4 Tape roll exchange =
141 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4. Tape roll exchange =
143 143  
144 144  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
145 145  
... ... @@ -167,7 +167,7 @@
167 167  {{layout-cell}}
168 168  ----
169 169  
170 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5 Chopper =
169 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5. Chopper =
171 171  
172 172  start chopper gui from terminal:
173 173  
... ... @@ -210,7 +210,7 @@
210 210  {{layout-cell}}
211 211  ----
212 212  
213 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6 Data collection =
212 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6. Data collection =
214 214  
215 215  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
216 216  
... ... @@ -343,7 +343,7 @@
343 343  {{layout-cell}}
344 344  ----
345 345  
346 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7 Troubleshooting =
345 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7. Troubleshooting =
347 347  
348 348  * (((
349 349  == {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==