Changes for page CFEL TapeDrive: Serial Crystallography
Last modified by chatzies on 2025-11-27 11:35
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... ... @@ -1,1 +1,1 @@ 1 - 08. Serial Crystallography: CFEL TapeDrive1 +14. Serial Crystallography: CFEL TapeDrive - Author
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... ... @@ -1,1 +1,1 @@ 1 -XWiki. tabermah1 +XWiki.ahenkel - Content
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... ... @@ -1,29 +1,83 @@ 1 1 {{layout}} 2 2 {{layout-section ac:type="single"}} 3 3 {{layout-cell}} 4 -[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]] 5 5 6 6 7 7 {{toc/}} 8 8 8 +[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]] 9 9 10 - ----10 +\\ 11 11 12 - = {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =12 +\\ 13 13 14 - suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.14 +\\ 15 15 16 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** === 17 + 18 +* remove vortex 19 +* beamstop to out-position? 20 +* remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01) 21 +* move cryojet out of the way, ramp the cryo up and shut it down. Close the compressed air to the shield flow. The biggest cable kinks and needs to be disconnected. 22 +* stop refill of the robot dewar and empty the dewar, warm up. 23 +* Disable backlight (unplug Festo connection). (% style="color: rgb(255,0,0);" %)//Is this still needed?// 24 +* (% style="color: rgb(0,0,0);" %)mount the 10 um diode and connect (via connector on the granite). This is connected to the leftmost Keithly (power up, check that settings are gain E06, Filter on, Rise time 1 ms. Keithly is connected to the oscilloscope and the delay generator, delay generator is connected to oscilloscope and detector, see diagram in P11 wiki. (% style="color: rgb(0, 0, 0); color: rgb(255, 0, 0)" %)//Insert here the setting for oscilloscope//(% style="color: rgb(0,0,0);" %)//. //Make sure to connect the multi-trigger to the back of the detector instead of the standard trigger, when starting to use the chopper (pull the thin ring with ‘grip’ area to detach). Cables are labeled. Make sure before using the chopper that the channels are enabled. 25 +* (% style="color: rgb(0,0,0);" %)move goniotower to X ~~ 17 000 26 + 27 +(% style="color: rgb(0,0,0);" %)Mounting of the TapeDrive is done by the CFEL group. Electronics and control units are combined on a small shelf, that requires only one power and network plug-in. The shelf will be placed left-sided (looking upstream) to the detector tower. The ElveFlow and control laptop as well as several necessary consumables are placed on a cart, which is to be positioned on the right (looking upstream) against the robot platform. The CFEL chiller is placed on the right of the platform and connected to the TapeDrive GASnozzle. 28 + 29 +\\ 30 + 31 +* adjust focus of the tape by moving X in goniometer tower until you see it in the overview camera. Final adjustment should be done when there is flow. Latest value is about 4500 in X and also 5000 in Z (higher soft limit changed from 2976,63 to 6000). (% style="color: rgb(255,0,0);" %)//Better to use the back illumination in the GUI to focus the tape - old instruction, not sure if this is the case. Back illumination is the external light source in the detector tower (the crystallography backlight should be disable for tapedrive).// 32 +* (% style="color: rgb(0,0,0);" %)Check the Minimum Detector Distance; the detector should not collide with the tapedrive while driven to 154 mm but it has to be verified. Also check that all cables and capillaries are taped away when the users start, so that nothing protrudes further than the tapedrive itself. Soft limit can be edited in eh.3.11 Atk panel. 33 +* (% style="color: rgb(0,0,0);" %)Reconfirm the alignment of the beamstop, once everything, including the nozzle, is in place. Modify config.ini for beamstop in- and out-position to both mach in (27 000). This way the beamstop will not be driven out when the hutch is opened, to avoid that the users forget to drive it in when restarting after entering the hutch. 34 + 35 +\\ 36 + 37 +Prepare necessary controls for data collection: 38 + 39 +* ATK panels: 40 +** p11/simplon_filewriter/eh.01 41 +** p11/simplon_detector/eh.01 42 +*** count time = 0.0075 43 +*** trigger (**ints **if working without the chopper, # or triggers is 1, **exts** if with the chopper, # or triggers = number of images). Exts is also the standard trigger in normal crystallography. If GUI is used to take images (e.g. for beamstop alignment) the trigger mode will be forced by that and needs to be reverted manually for the users to continue. 44 +*** numer of images = 100 000 45 +*** number of images in container = 1000 46 +*** mode 16m/4M 47 +*** detector distance = 200 mm (usually) 48 +** p11/detectortower 49 +** p11/motor/eh.09 50 +* remote access to TapeDrive control laptop: 51 +** open terminal, execute command: xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611 52 + password: //written on control laptop 53 +// 54 +* Cameras: 55 +** haspp11ethcam1 → detector tower camera to monitor nozzle 56 +** haspp11ethcam4 → goniometer camera to monitor tape from above 57 +* OnDa (on haspp11user04): 58 +//This starts both GUIs (Frame Viewer & Crystallography GUI) and onda-monitor, the server component. The tool has a bunch of parameters which you can list with ./onda-runner.py —help.//\\ 59 +** 16M mode → cd /home/p11user/CfelSoft/202103_onda 60 + ./onda-runner.py onda-config/onda-config-Eiger16M.toml 61 +** 4M mode → cd /home/p11user/CfelSoft/202103_onda 62 + ./onda-runner.py onda-config/onda-config-Eiger4M.toml\\ 63 + 64 +\\ 65 + 66 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** === 67 + 68 +suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc. 69 + 16 16 Preparation: 17 17 18 -* filter through 30 µm cell filter (green) or 50 µm (yellow)into Eppendorf or Falcon (original!) tube72 +* filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube 19 19 20 20 [[image:attach:20211201_095534.jpg||height="250"]] 21 21 76 +\\ 22 22 23 ----- 78 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange 79 +** === 24 24 25 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange = 26 - 27 27 After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!** 28 28 29 29 Be aware to which channel and nozzle line your sample is connected. ... ... @@ -38,45 +38,44 @@ 38 38 39 39 [[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]] 40 40 95 +\\ 41 41 42 ----- 97 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation 98 +** === 43 43 44 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3. TapeDrive operation = 45 - 46 -The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window. 47 - 100 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window. 48 48 Now start the client. Same thing, a terminal will open, shortly after a small window. 49 49 50 50 Click 'Devices' in the **client session** to open the control window. 51 51 [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]] 52 52 53 - == {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}Tape ==106 +**Tape** 54 54 55 -* Click the 'main tape' icon 108 +* Click on the 'main tape' icon 56 56 * Change the velocity by typing anything from **0.3-3.0 mm/s** 57 57 * Common velocity is 1.0-1.5 mm/s 58 58 * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point. 59 - 112 +\\ 60 60 * [[image:attach:kamzik_tape.PNG||height="400"]] 61 61 62 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle == 115 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ==== 63 63 64 -* Click on the 'nozzle' icon.117 +* Click an the 'nozzle' icon. 65 65 * ((( 66 66 The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully. 67 67 The absolute position is **not calibrated** and therefore changes after each restart of kamzik. 68 68 69 69 70 -(% style="color: #ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:#ff0000" %)**!!!**(%%)123 +(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%) 71 71 \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments. 72 72 73 73 (% class="wrapped" %) 74 74 |=((( 75 - 128 +\\ 76 76 )))|=((( 77 77 direction 78 78 )))|=((( 79 -recommended step size 132 +recommended step size 80 80 for visual adjustment 81 81 )))|=(% colspan="1" %)((( 82 82 recommended step size ... ... @@ -111,31 +111,37 @@ 111 111 be careful with -z, tape could be pushed out of focus 112 112 ))) 113 113 114 - 167 +** 168 +\\** 115 115 ))) 116 116 * [[image:attach:kamzik_nozzle.PNG||height="400"]] 117 117 118 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller == 172 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ==== 119 119 120 120 * The chiller circulates fluid through the gas nozzle. 121 121 122 - == {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}Gas nozzle ==176 +\\ 123 123 178 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ==== 124 124 125 - == {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}IR camera ==180 +\\ 126 126 182 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ==== 127 127 184 +\\ 185 + 128 128 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command: 129 129 130 130 xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611 131 - password: //written on control laptop (changes every 6 months)// 189 + password: //written on control laptop (changes every 6 months) 190 +// 132 132 192 +\\ 133 133 194 +\\ 134 134 135 -- ---196 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** === 136 136 137 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4. Tape roll exchange = 138 - 139 139 The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s). 140 140 141 141 * Remove the (almost) empty feeder roll by pulling it upwards. ... ... @@ -143,7 +143,7 @@ 143 143 * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm** 144 144 * Cut the first cm of the new tape roll, directly behind the black sticky part. 145 145 * Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll. 146 -* Open magnetic tape stabilizer on the **left side**. (% style="color:#ff0000" %)//!!!BEAMSTOP!!!//205 +* Open magnetic tape stabilizer on the **left side**. //!!!BEAMSTOP!!!// 147 147 * Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s). 148 148 * Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded. 149 149 * Attach new tape to the empty roll by using some sticky tape. ... ... @@ -150,112 +150,63 @@ 150 150 * Close tape stabilizer. 151 151 * Reset tape counter in kamzik by clicking "<Call>" in "Main-Tape". 152 152 153 -**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]** 212 +**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]] 213 +** 154 154 215 +\\ 155 155 217 +\\ 156 156 157 - 158 -{{/layout-cell}} 159 -{{/layout-section}} 219 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}**14.6 Nozzle exchange** === 160 160 161 -{{layout-section ac:type="single"}} 162 -{{layout-cell}} 163 ----- 221 +\\ 164 164 165 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14. 5Chopper"/}}8.5.Chopper=223 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Datacollection"/}}**14.7 Data collection** === 166 166 167 -st art chopperguifromterminal:225 +Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command: 168 168 169 -(% class="code" %) 170 -((( 171 - cd /gpfs/local/shared/ChopperControl 172 -))) 227 + xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611 228 + password: //written on control laptop (changes every 6 months)// 173 173 174 -(% class="code" %) 175 -((( 176 - python chopperControl.py 177 -\\ 178 -))) 179 -{{/layout-cell}} 180 -{{/layout-section}} 230 + //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password// 181 181 182 -{{layout-section ac:type="two_equal"}} 183 -{{layout-cell}} 184 - [[image:attach:20211212_233140.jpg||height="250"]] 185 -{{/layout-cell}} 232 +\\ 186 186 187 -{{layout-cell}} 188 -**Chopper → no Chopper** 234 +**Check list:** 189 189 190 -* decrease frequency by max. 10 Hz steps 191 -** click arrow down 10x, "Set", arrow down 10x, "Set", .... 192 -* at frequency = 10 Hz, click "Stop" 193 -* set "Hole" to <none> (% style="color:#ff0000" %)//Takes some minutes// 236 +* Open BS1 (//CrystalControl)// 237 +* Put collimator in (//CrystalControl)// 238 +* Check beamstop position = 27 000 (//Atk panel p11/motor/eh.3.09)// 239 +* Open fast shutter (//CrystalControl)// 240 +* Check diode voltage ~~ 0.0 (//kamzik Run/Diode voltage)// 241 +* Close fast shutter (//CrystalControl)// 194 194 195 - (% style="color:#000000" %)**no Chopper → Chopper**243 +\\ 196 196 197 -* (% style="color:#000000" %)set "Hole" to <Inner hole (largest)> (% style="color:#ff0000" %)//Takes some minutes// 198 -* (% style="color:#000000" %)increase frequency by max. 10 Hz steps 199 -** (% style="color:#000000" %)click arrow up 10x, "Set", arrow up 10x, "Set", ..... 200 -* (% style="color:#000000" %)//usually 130 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 130 Hz// 201 -{{/layout-cell}} 202 -{{/layout-section}} 245 +**Start run in kamzik 246 +** 203 203 204 -{{layout-section ac:type="single"}} 205 -{{layout-cell}} 206 ----- 248 +Click "Start" icon in kamzik. 207 207 208 - = {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6. Data collection=250 +[[image:attach:kamzik_run-tile.jpg||height="400"]] 209 209 210 -Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command: 211 - 212 -(% class="code" %) 213 -((( 214 - xfreerdp +clipboard /size:1920x1080 /u:cfelusr /v:cfeld-tapetop 215 - password: written on control laptop (changes every 6 months) 216 -))) 217 - 218 - //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password// 219 - 220 - 252 +---- 221 221 {{/layout-cell}} 222 222 {{/layout-section}} 223 223 224 -{{layout-section ac:type="two_ left_sidebar"}}256 +{{layout-section ac:type="two_equal"}} 225 225 {{layout-cell}} 226 - **Check list:**258 +\\ 227 227 228 -(% style="color:#ff0000" %)//always after entering the hutch!// 229 - 230 -(% style="color:#000000" %)Click "Run" icon in kamzik. 231 - 232 -* Put detector distance 200 in //prepare_for_measurement ( )// 233 -* Click "<Call>" next to //prepare_for_measurement// 234 -This will: 235 -** drive the collimator in //IN// position 236 -** drive detector to 200 mm 237 - 238 -* Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed // 239 - 240 -//**Set run parameters and go 🏃💥**// 241 - 242 242 1. Set your desired No. of frames 243 -1. Enter the protein ID of your crystal sample 244 -1. Match exposure time with chopper setting or set desired exposure time 261 +1. Match exposure time with chopper setting 245 245 1. Check trigger setting and match to chopper setting 246 -no chopper → external triffer //off// 247 - chopper → external trigger// on// 248 -1. To start the run, click "<Call>" next to //start_run (exposure )// 263 +1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it. 264 +1. To start the run, click "<Call>" next to //exposure time// 249 249 {{/layout-cell}} 250 250 251 251 {{layout-cell}} 252 -[[image:attach:Kamzik_run(2).PNG]] 253 -{{/layout-cell}} 254 -{{/layout-section}} 255 - 256 -{{layout-section ac:type="single"}} 257 -{{layout-cell}} 258 -(% class="relative-table wrapped" style="width:55.9569%" %) 268 +(% class="wrapped" %) 259 259 |=((( 260 260 Parameter 261 261 )))|=((( ... ... @@ -266,7 +266,7 @@ 266 266 |((( 267 267 Images number 268 268 )))|((( 269 -number of frames you want to collect 279 +Set number of frames you want to collect 270 270 )))|(% colspan="1" %)((( 271 271 usually 100k - 200k, but adjust according to hitrate 272 272 ... ... @@ -277,9 +277,12 @@ 277 277 )))|((( 278 278 exposure time of Eiger 279 279 280 - 290 +\\ 281 281 )))|(% colspan="1" %)((( 282 -with chopper → opening time displayed in chopper GUI 292 +chopper at 133 Hz → 3.7 ms 293 + 294 +chopper at 66 Hz → 7.7 ms 295 + 283 283 no chopper → 7.7 ms 284 284 285 285 ---- ... ... @@ -289,7 +289,7 @@ 289 289 )))|((( 290 290 changes automatically 291 291 )))|(% colspan="1" %)((( 292 - 305 +\\ 293 293 ))) 294 294 |(% colspan="1" %)((( 295 295 Detector distance ... ... @@ -296,109 +296,38 @@ 296 296 )))|(% colspan="1" %)((( 297 297 current detector postion readout 298 298 )))|(% colspan="1" %)((( 299 - 312 +\\ 300 300 ))) 301 301 |(% colspan="1" %)((( 302 - proteinID315 +move_detector (distance) 303 303 )))|(% colspan="1" %)((( 304 - proteinID ofcrystal sample(see AMARCORD)317 +Set detector distance 305 305 )))|(% colspan="1" %)((( 306 - 319 +200.0 mm (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>" 307 307 ))) 308 - 309 ----- 321 +|(% colspan="1" %)((( 322 +Diode voltage 323 +)))|(% colspan="1" %)((( 324 +read out of diode 325 +)))|(% colspan="1" %)((( 326 +**~~ 0 !!! higher values indicate missaligned beam stop!!!** 327 +))) 310 310 {{/layout-cell}} 311 311 {{/layout-section}} 312 312 313 -{{layout-section ac:type="two_equal"}} 314 -{{layout-cell}} 315 -**After data collection/Before entering the exp. hutch:** 316 - 317 -1. Go to "Safety" in kamzik. 318 -1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically: 319 -11. Drive the collimator out 320 -11. Drive the detector back to 1500 mm 321 -1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left). 322 - 323 -[[image:attach:Screenshot from 2022-04-04 19-36-29.png]] 324 -{{/layout-cell}} 325 - 326 -{{layout-cell}} 327 - 328 - 329 - 330 - 331 - 332 - 333 -[[image:attach:safety_kamzik.PNG]] 334 -{{/layout-cell}} 335 -{{/layout-section}} 336 - 337 337 {{layout-section ac:type="single"}} 338 338 {{layout-cell}} 339 -- ---333 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Troubleshooting"/}}14.8 Troubleshooting === 340 340 341 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7. Troubleshooting = 342 - 343 343 * ((( 344 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped == 345 - 346 -* Tape roll empty? 347 -* motor connected (icon green)? 336 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==== 348 348 ))) 349 - 350 350 * ((( 351 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding == 352 - 353 -* displayed in green=connected? 354 -* nozzle holder colliding somewhere? 355 -* motor limits reached? → position displayed in red 339 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ==== 356 356 ))) 357 - 358 358 * ((( 359 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure == 360 - 361 -* channel turned on? 362 -* Sample empty? 363 -* Nozzle or lines clogged? → flush with water, change nozzle 342 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ==== 364 364 ))) 365 - 366 -* ((( 367 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues == 368 - 369 -* First, restart kamzik (server+client) and see if this solves the issue 370 -* If not, restart the Simplon server (in Astor → haspp11exp03, right click on Simplon/EH.1 and restart) 371 -* ((( 372 -If nothing works, kill the Simplon process and then start the server again:* 373 - 374 -((( 375 -(% class="code" %) 376 -((( 377 -ssh haspp11exp03 378 -))) 379 -))) 380 - 381 -* ((( 382 -(% class="code" %) 383 -((( 384 -ps -ef | grep Simplon 385 -))) 386 -))) 387 -* ((( 388 -(% class="code" %) 389 -((( 390 -kill -9 simplon_process_id 391 -))) 392 -))) 393 -))) 394 -* After the Simplon server is back up, close all kamzik windows and restart (first server, then client) 395 -))) 396 396 {{/layout-cell}} 397 397 {{/layout-section}} 398 - 399 -{{layout-section ac:type="single"}} 400 -{{layout-cell}} 401 - 402 -{{/layout-cell}} 403 -{{/layout-section}} 404 404 {{/layout}}
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