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Last modified by chatzies on 2025-11-27 11:35
From version 82.1
edited by tabermah
on 2024-12-10 15:22
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To version 84.2
edited by chatzies
on 2025-11-20 09:44
Change comment: allow view right for XWiki.chatzies

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11 11  
12 12  = {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =
13 13  
14 -suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
14 +* Suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
15 15  
16 16  Preparation:
17 17  
18 -* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
18 +* filter through 30 µm (green) or 50 µm (yellow) cell filter into an Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
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24 24  
25 25  = {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
26 26  
27 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
27 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish tube goes all the way to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
28 28  
29 29  Be aware to which channel and nozzle line your sample is connected.
30 30  
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57 57  * Common velocity is 1.0-1.5 mm/s
58 58  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
59 59  
60 -* [[image:attach:kamzik_tape.PNG||height="400"]]
61 61  
61 +[[image:attach:kamzik_tape.PNG||height="400"]]
62 +
63 +
62 62  == {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle ==
63 63  
64 64  * Click on the 'nozzle' icon.
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67 67  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
68 68  
69 69  
70 -(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
72 +(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving the nozzle, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
71 71  \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
72 72  
73 73  (% class="wrapped" %)
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113 113  
114 114  
115 115  )))
116 -* [[image:attach:kamzik_nozzle.PNG||height="400"]]
117 117  
119 +[[image:attach:kamzik_nozzle.PNG||height="400"]]
120 +
121 +
118 118  == {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller ==
119 119  
120 120  * The chiller circulates fluid through the gas nozzle.
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152 152  
153 153  **[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]**
154 154  
155 -
156 -
157 157  
158 158  {{/layout-cell}}
159 159  {{/layout-section}}
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229 229  
230 230  (% style="color:#000000" %)Click "Run" icon in kamzik.
231 231  
232 -* Put detector distance 200 in //prepare_for_measurement (      )//
234 +* Put detector distance 200 mm in //prepare_for_measurement (      )//
233 233  * Click "<Call>" next to //prepare_for_measurement//
234 234  This will:
235 235  ** drive the collimator in //IN// position
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326 326  {{layout-cell}}
327 327  
328 328  
329 -
330 -
331 -
332 -
333 333  [[image:attach:safety_kamzik.PNG]]
334 334  {{/layout-cell}}
335 335  {{/layout-section}}
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395 395  )))
396 396  {{/layout-cell}}
397 397  {{/layout-section}}
398 -
399 -{{layout-section ac:type="single"}}
400 -{{layout-cell}}
401 -
402 -{{/layout-cell}}
403 -{{/layout-section}}
404 404  {{/layout}}
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