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Last modified by chatzies on 2025-11-27 11:35
From version 83.1
edited by tabermah
on 2024-12-10 15:24
Change comment: There is no comment for this version
To version 79.6
edited by tabermah
on 2024-12-10 15:20
Change comment: There is no comment for this version

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... ... @@ -9,13 +9,13 @@
9 9  
10 10  ----
11 11  
12 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =
12 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1 Sample characteristics and preparation =
13 13  
14 -* Suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
14 +suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
15 15  
16 16  Preparation:
17 17  
18 -* filter through 30 µm cell filter (green) or 50 µm (yellow) into an Eppendorf or Falcon (original!) tube
18 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
... ... @@ -22,8 +22,11 @@
22 22  
23 23  ----
24 24  
25 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
25 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2 Connecting sample to ElveFlow, sample exchange =
26 26  
27 +*
28 +** ==
29 +
27 27  After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
28 28  
29 29  Be aware to which channel and nozzle line your sample is connected.
... ... @@ -41,10 +41,12 @@
41 41  
42 42  ----
43 43  
44 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3. TapeDrive operation =
47 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3 TapeDrive operation =
45 45  
46 -​​​​​​The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
49 +*
50 +** ==
47 47  
52 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
48 48  Now start the client. Same thing, a terminal will open, shortly after a small window.
49 49  
50 50  Click 'Devices' in the **client session** to open the control window.
... ... @@ -134,7 +134,7 @@
134 134  
135 135  ----
136 136  
137 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4. Tape roll exchange =
142 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4 Tape roll exchange =
138 138  
139 139  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
140 140  
... ... @@ -162,7 +162,7 @@
162 162  {{layout-cell}}
163 163  ----
164 164  
165 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5. Chopper =
170 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5 Chopper =
166 166  
167 167  start chopper gui from terminal:
168 168  
... ... @@ -205,7 +205,7 @@
205 205  {{layout-cell}}
206 206  ----
207 207  
208 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6. Data collection =
213 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6 Data collection =
209 209  
210 210  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
211 211  
... ... @@ -338,7 +338,7 @@
338 338  {{layout-cell}}
339 339  ----
340 340  
341 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7. Troubleshooting =
346 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7 Troubleshooting =
342 342  
343 343  * (((
344 344  == {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==
... ... @@ -356,7 +356,7 @@
356 356  )))
357 357  
358 358  * (((
359 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ==
364 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
360 360  
361 361  * channel turned on?
362 362  * Sample empty?
... ... @@ -364,7 +364,7 @@
364 364  )))
365 365  
366 366  * (((
367 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ==
372 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ====
368 368  
369 369  * First, restart kamzik (server+client) and see if this solves the issue
370 370  * If not, restart the Simplon server (in Astor → haspp11exp03, right click on Simplon/EH.1 and restart)