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Last modified by chatzies on 2025-11-27 11:35
From version 83.3
edited by tabermah
on 2024-12-10 15:50
Change comment: There is no comment for this version
To version 79.5
edited by tabermah
on 2024-12-10 15:19
Change comment: There is no comment for this version

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... ... @@ -9,13 +9,13 @@
9 9  
10 10  ----
11 11  
12 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =
12 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1 Sample characteristics and preparation =
13 13  
14 -* Suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
14 +suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
15 15  
16 16  Preparation:
17 17  
18 -* filter through 30 µm (green) or 50 µm (yellow) cell filter into an Eppendorf or Falcon (original!) tube
18 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
... ... @@ -22,10 +22,13 @@
22 22  
23 23  ----
24 24  
25 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
25 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2 Connecting sample to ElveFlow, sample exchange =
26 26  
27 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish tube goes all the way to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
27 +*
28 +** ==
28 28  
30 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
31 +
29 29  Be aware to which channel and nozzle line your sample is connected.
30 30  
31 31   Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
... ... @@ -41,10 +41,12 @@
41 41  
42 42  ----
43 43  
44 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3. TapeDrive operation =
47 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3 TapeDrive operation =
45 45  
46 -​​​​​​The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
49 +*
50 +** ==
47 47  
52 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
48 48  Now start the client. Same thing, a terminal will open, shortly after a small window.
49 49  
50 50  Click 'Devices' in the **client session** to open the control window.
... ... @@ -57,10 +57,8 @@
57 57  * Common velocity is 1.0-1.5 mm/s
58 58  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
59 59  
65 +* [[image:attach:kamzik_tape.PNG||height="400"]]
60 60  
61 -[[image:attach:kamzik_tape.PNG||height="400"]]
62 -
63 -
64 64  == {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle ==
65 65  
66 66  * Click on the 'nozzle' icon.
... ... @@ -69,7 +69,7 @@
69 69  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
70 70  
71 71  
72 -(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving the nozzle, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
75 +(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
73 73  \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
74 74  
75 75  (% class="wrapped" %)
... ... @@ -115,11 +115,8 @@
115 115  
116 116  
117 117  )))
121 +* [[image:attach:kamzik_nozzle.PNG||height="400"]]
118 118  
119 -[[image:attach:kamzik_nozzle.PNG||height="400"]]
120 -
121 -== ==
122 -
123 123  == {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller ==
124 124  
125 125  * The chiller circulates fluid through the gas nozzle.
... ... @@ -127,7 +127,7 @@
127 127  == {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}Gas nozzle ==
128 128  
129 129  
130 -== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}IR camera ==
130 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}IR camera ====
131 131  
132 132  
133 133  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
... ... @@ -139,7 +139,7 @@
139 139  
140 140  ----
141 141  
142 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4. Tape roll exchange =
142 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}**8.4 Tape roll exchange** ==
143 143  
144 144  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
145 145  
... ... @@ -167,7 +167,7 @@
167 167  {{layout-cell}}
168 168  ----
169 169  
170 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5. Chopper =
170 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}**8.5 Chopper** ==
171 171  
172 172  start chopper gui from terminal:
173 173  
... ... @@ -210,7 +210,7 @@
210 210  {{layout-cell}}
211 211  ----
212 212  
213 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6. Data collection =
213 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**8.6 Data collection** ==
214 214  
215 215  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
216 216  
... ... @@ -343,10 +343,10 @@
343 343  {{layout-cell}}
344 344  ----
345 345  
346 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7. Troubleshooting =
346 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}**8.7 Troubleshooting** ==
347 347  
348 348  * (((
349 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==
349 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
350 350  
351 351  * Tape roll empty?
352 352  * motor connected (icon green)?
... ... @@ -353,7 +353,7 @@
353 353  )))
354 354  
355 355  * (((
356 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ==
356 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
357 357  
358 358  * displayed in green=connected?
359 359  * nozzle holder colliding somewhere?
... ... @@ -361,7 +361,7 @@
361 361  )))
362 362  
363 363  * (((
364 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ==
364 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
365 365  
366 366  * channel turned on?
367 367  * Sample empty?
... ... @@ -369,7 +369,7 @@
369 369  )))
370 370  
371 371  * (((
372 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ==
372 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ====
373 373  
374 374  * First, restart kamzik (server+client) and see if this solves the issue
375 375  * If not, restart the Simplon server (in Astor → haspp11exp03, right click on Simplon/EH.1 and restart)