Skip to Content
Last modified by chatzies on 2025-11-27 11:35
From version 83.3
edited by tabermah
on 2024-12-10 15:50
Change comment: There is no comment for this version
To version 83.1
edited by tabermah
on 2024-12-10 15:24
Change comment: There is no comment for this version

Summary

Details

Page properties
Content
... ... @@ -15,7 +15,7 @@
15 15  
16 16  Preparation:
17 17  
18 -* filter through 30 µm (green) or 50 µm (yellow) cell filter into an Eppendorf or Falcon (original!) tube
18 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into an Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
... ... @@ -24,7 +24,7 @@
24 24  
25 25  = {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
26 26  
27 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish tube goes all the way to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
27 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
28 28  
29 29  Be aware to which channel and nozzle line your sample is connected.
30 30  
... ... @@ -57,10 +57,8 @@
57 57  * Common velocity is 1.0-1.5 mm/s
58 58  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
59 59  
60 +* [[image:attach:kamzik_tape.PNG||height="400"]]
60 60  
61 -[[image:attach:kamzik_tape.PNG||height="400"]]
62 -
63 -
64 64  == {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle ==
65 65  
66 66  * Click on the 'nozzle' icon.
... ... @@ -69,7 +69,7 @@
69 69  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
70 70  
71 71  
72 -(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving the nozzle, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
70 +(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
73 73  \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
74 74  
75 75  (% class="wrapped" %)
... ... @@ -115,11 +115,8 @@
115 115  
116 116  
117 117  )))
116 +* [[image:attach:kamzik_nozzle.PNG||height="400"]]
118 118  
119 -[[image:attach:kamzik_nozzle.PNG||height="400"]]
120 -
121 -== ==
122 -
123 123  == {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller ==
124 124  
125 125  * The chiller circulates fluid through the gas nozzle.