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Last modified by chatzies on 2025-11-27 11:35
From version 83.4
edited by tabermah
on 2024-12-10 15:50
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To version 36.1
edited by ahenkel
on 2021-12-12 22:53
Change comment: There is no comment for this version

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Title
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1 -08. Serial Crystallography: CFEL TapeDrive
1 +14. Serial Crystallography: CFEL TapeDrive
Author
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1 -XWiki.tabermah
1 +XWiki.ahenkel
Content
... ... @@ -1,31 +5,82 @@
1 -{{layout}}
2 -{{layout-section ac:type="single"}}
3 -{{layout-cell}}
4 -[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
5 5  
6 6  
7 7  {{toc/}}
8 8  
5 +[[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
9 9  
10 -----
7 +\\
11 11  
12 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =
9 +\\
13 13  
14 -* Suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
11 +\\
15 15  
13 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1RevertfromCrystallographytoTapeDrive(P11staff/CFEL)"/}}**14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)** ===
14 +
15 +* remove vortex
16 +* beamstop to out-position?
17 +* remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
18 +* move cryojet out of the way, ramp the cryo up and shut it down. Close the compressed air to the shield flow. The biggest cable kinks and needs to be disconnected.
19 +* stop refill of the robot dewar and empty the dewar, warm up.
20 +* Disable backlight (unplug Festo connection). (% style="color: rgb(255,0,0);" %)//Is this still needed?//
21 +* (% style="color: rgb(0,0,0);" %)mount the 10 um diode and connect (via connector on the granite). This is connected to the leftmost Keithly (power up, check that settings are gain E06, Filter on, Rise time 1 ms. Keithly is connected to the oscilloscope and the delay generator, delay generator is connected to oscilloscope and detector, see diagram in P11 wiki. (% style="color: rgb(0, 0, 0); color: rgb(255, 0, 0)" %)//Insert here the setting for oscilloscope//(% style="color: rgb(0,0,0);" %)//. //Make sure to connect the multi-trigger to the back of the detector instead of the standard trigger, when starting to use the chopper (pull the thin ring with ‘grip’ area to detach). Cables are labeled. Make sure before using the chopper that the channels are enabled.
22 +* (% style="color: rgb(0,0,0);" %)move goniotower to X ~~ 17 000
23 +
24 +(% style="color: rgb(0,0,0);" %)Mounting of the TapeDrive is done by the CFEL group. Electronics and control units are combined on a small shelf, that requires only one power and network plug-in. The shelf will be placed left-sided (looking upstream) to the detector tower. The ElveFlow and control laptop as well as several necessary consumables are placed on a cart, which is to be positioned on the right (looking upstream) against the robot platform. The CFEL chiller is placed on the right of the platform and connected to the TapeDrive GASnozzle.
25 +
26 +\\
27 +
28 +* adjust focus of the tape by moving X in goniometer tower until you see it in the overview camera. Final adjustment should be done when there is flow. Latest value is about 4500 in X and also 5000 in Z (higher soft limit changed from 2976,63 to 6000). (% style="color: rgb(255,0,0);" %)//Better to use the back illumination in the GUI to focus the tape - old instruction, not sure if this is the case. Back illumination is the external light source in the detector tower (the crystallography backlight should be disable for tapedrive).//
29 +* (% style="color: rgb(0,0,0);" %)Check the Minimum Detector Distance; the detector should not collide with the tapedrive while driven to 154 mm but it has to be verified. Also check that all cables and capillaries are taped away when the users start, so that nothing protrudes further than the tapedrive itself. Soft limit can be edited in eh.3.11 Atk panel.
30 +* (% style="color: rgb(0,0,0);" %)Reconfirm the alignment of the beamstop, once everything, including the nozzle, is in place. Modify config.ini for beamstop in- and out-position to both mach in (27 000). This way the beamstop will not be driven out when the hutch is opened, to avoid that the users forget to drive it in when restarting after entering the hutch.
31 +
32 +\\
33 +
34 +Prepare necessary controls for data collection:
35 +
36 +* ATK panels:
37 +** p11/simplon_filewriter/eh.01
38 +** p11/simplon_detector/eh.01
39 +*** count time = 0.0075
40 +*** trigger (**ints **if working without the chopper, # or triggers is 1, **exts** if with the chopper, # or triggers = number of images). Exts is also the standard trigger in normal crystallography. If GUI is used to take images (e.g. for beamstop alignment) the trigger mode will be forced by that and needs to be reverted manually for the users to continue.
41 +*** numer of images = 100 000
42 +*** number of images in container = 1000
43 +*** mode 16m/4M
44 +*** detector distance = 200 mm (usually)
45 +** p11/detectortower
46 +** p11/motor/eh.09
47 +* remote access to TapeDrive control laptop:
48 +** open terminal, execute command:          xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
49 + password: //written on control laptop
50 +//
51 +* Cameras:
52 +** haspp11ethcam1 → detector tower camera to monitor nozzle
53 +** haspp11ethcam4 →  goniometer camera to monitor tape from above
54 +* OnDa (on haspp11user04): 
55 +//This starts both GUIs (Frame Viewer & Crystallography GUI) and onda-monitor, the server component. The tool has a bunch of parameters which you can list with ./onda-runner.py —help.//\\
56 +** 16M mode →    cd /home/p11user/CfelSoft/202103_onda
57 + ./onda-runner.py onda-config/onda-config-Eiger16M.toml
58 +** 4M mode   →    cd /home/p11user/CfelSoft/202103_onda
59 + ./onda-runner.py onda-config/onda-config-Eiger4M.toml\\
60 +
61 +\\
62 +
63 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2Samplecharacteristicsandpreparation"/}}**14.2 Sample characteristics and preparation** ===
64 +
65 +suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
66 +
16 16  Preparation:
17 17  
18 -* filter through 30 µm (green) or 50 µm (yellow) cell filter into an Eppendorf or Falcon (original!) tube
69 +* filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
73 +\\
22 22  
23 -----
75 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3ConnectingsampletoElveFlow,sampleexchange"/}}**14.3 Connecting sample to ElveFlow, sample exchange
76 +** ===
24 24  
25 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
78 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
26 26  
27 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish tube goes all the way to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
28 -
29 29  Be aware to which channel and nozzle line your sample is connected.
30 30  
31 31   Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
... ... @@ -38,47 +38,44 @@
38 38  
39 39  [[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]]
40 40  
92 +\\
41 41  
42 -----
94 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4TapeDriveoperation"/}}**14.4 TapeDrive operation
95 +** ===
43 43  
44 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3. TapeDrive operation =
45 -
46 -​​​​​​The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
47 -
97 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
48 48  Now start the client. Same thing, a terminal will open, shortly after a small window.
49 49  
50 50  Click 'Devices' in the **client session** to open the control window.
51 51  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
52 52  
53 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}Tape ==
103 +**Tape**
54 54  
55 -* Click the 'main tape' icon
105 +* Click on the 'main tape' icon
56 56  * Change the velocity by typing anything from **0.3-3.0 mm/s**
57 57  * Common velocity is 1.0-1.5 mm/s
58 58  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
59 -
109 +\\
110 +* [[image:attach:kamzik_tape.PNG||height="400"]]
60 60  
61 -[[image:attach:kamzik_tape.PNG||height="400"]]
112 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
62 62  
63 -
64 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle ==
65 -
66 -* Click on the 'nozzle' icon.
114 +* Click an the 'nozzle' icon.
67 67  * (((
68 68  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
69 69  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
70 70  
71 71  
72 -(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving the nozzle, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
120 +(% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
73 73  \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
74 74  
75 75  (% class="wrapped" %)
76 76  |=(((
77 -
125 +\\
78 78  )))|=(((
79 79  direction
80 80  )))|=(((
81 -recommended step size
129 +recommended step size
82 82  for visual adjustment
83 83  )))|=(% colspan="1" %)(((
84 84  recommended step size
... ... @@ -113,33 +113,37 @@
113 113  be careful with -z, tape could be pushed out of focus
114 114  )))
115 115  
116 -
164 +**
165 +\\**
117 117  )))
167 +* [[image:attach:kamzik_nozzle.PNG||height="400"]]
118 118  
119 -[[image:attach:kamzik_nozzle.PNG||height="400"]]
169 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
120 120  
121 -
122 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller ==
123 -
124 124  * The chiller circulates fluid through the gas nozzle.
125 125  
126 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}Gas nozzle ==
173 +\\
127 127  
175 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ====
128 128  
129 -== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}IR camera ==
177 +\\
130 130  
179 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ====
131 131  
181 +\\
182 +
132 132  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
133 133  
134 134   xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
135 - password: //written on control laptop (changes every 6 months)//
186 + password: //written on control laptop (changes every 6 months)
187 +//
136 136  
189 +\\
137 137  
191 +\\
138 138  
139 -----
193 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ===
140 140  
141 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4. Tape roll exchange =
142 -
143 143  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
144 144  
145 145  * Remove the (almost) empty feeder roll by pulling it upwards.
... ... @@ -147,7 +147,7 @@
147 147  * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
148 148  * Cut the first cm of the new tape roll, directly behind the black sticky part.
149 149  * Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll.
150 -* Open magnetic tape stabilizer on the **left side**. (% style="color:#ff0000" %)//!!!BEAMSTOP!!!//
202 +* Open magnetic tape stabilizer on the **left side**. //!!!BEAMSTOP!!!//
151 151  * Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s).
152 152  * Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded.
153 153  * Attach new tape to the empty roll by using some sticky tape.
... ... @@ -154,112 +154,46 @@
154 154  * Close tape stabilizer.
155 155  * Reset tape counter in kamzik by clicking "<Call>" in "Main-Tape".
156 156  
157 -**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]**
209 +**[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]
210 +**
158 158  
212 +\\
159 159  
214 +\\
160 160  
161 -
162 -{{/layout-cell}}
163 -{{/layout-section}}
216 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Nozzleexchange"/}}**14.6 Nozzle exchange** ===
164 164  
165 -{{layout-section ac:type="single"}}
166 -{{layout-cell}}
167 -----
218 +\\
168 168  
169 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5. Chopper =
220 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Datacollection"/}}**14.7 Data collection** ===
170 170  
171 -start chopper gui from terminal:
172 -
173 -(% class="code" %)
174 -(((
175 - cd /gpfs/local/shared/ChopperControl          
176 -)))
177 -
178 -(% class="code" %)
179 -(((
180 - python chopperControl.py
181 -\\
182 -)))
183 -{{/layout-cell}}
184 -{{/layout-section}}
185 -
186 -{{layout-section ac:type="two_equal"}}
187 -{{layout-cell}}
188 - [[image:attach:20211212_233140.jpg||height="250"]]
189 -{{/layout-cell}}
190 -
191 -{{layout-cell}}
192 -**Chopper → no Chopper**
193 -
194 -* decrease frequency by max. 10 Hz steps
195 -** click arrow down 10x, "Set", arrow down 10x, "Set", ....
196 -* at frequency = 10 Hz, click "Stop"
197 -* set "Hole" to <none> (% style="color:#ff0000" %)//Takes some minutes//
198 -
199 -(% style="color:#000000" %)**no Chopper → Chopper**
200 -
201 -* (% style="color:#000000" %)set "Hole" to <Inner hole (largest)> (% style="color:#ff0000" %)//Takes some minutes//
202 -* (% style="color:#000000" %)increase frequency by max. 10 Hz steps
203 -** (% style="color:#000000" %)click arrow up 10x, "Set", arrow up 10x, "Set", .....
204 -* (% style="color:#000000" %)//usually 130 Hz, results in exposure time of 3.7 ms (technical exposure will always be 7.7 ms, since max. frame rate is 130 Hz//
205 -{{/layout-cell}}
206 -{{/layout-section}}
207 -
208 -{{layout-section ac:type="single"}}
209 -{{layout-cell}}
210 -----
211 -
212 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6. Data collection =
213 -
214 214  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
215 215  
216 -(% class="code" %)
217 -(((
218 - xfreerdp +clipboard /size:1920x1080 /u:cfelusr /v:cfeld-tapetop
219 - password: written on control laptop (changes every 6 months)
220 -)))
224 + xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
225 + password: //written on control laptop (changes every 6 months)//
221 221  
222 - //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
227 + //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
223 223  
224 -
225 -{{/layout-cell}}
226 -{{/layout-section}}
229 +\\
227 227  
228 -{{layout-section ac:type="two_left_sidebar"}}
229 -{{layout-cell}}
230 230  **Check list:**
231 231  
232 -(% style="color:#ff0000" %)//always after entering the hutch!//
233 +* Open BS1  (//CrystalControl)//
234 +* Put collimator in (//CrystalControl)//
235 +* Check beamstop position = 27 000 (//Atk panel p11/motor/eh.3.09)//
236 +* Open fast shutter (//CrystalControl)//
237 +* Check diode voltage ~~ 0.0 (//kamzik Run/Diode voltage)//
238 +* Close fast shutter (//CrystalControl)//
233 233  
234 -(% style="color:#000000" %)Click "Run" icon in kamzik.
240 +\\
235 235  
236 -* Put detector distance 200 in //prepare_for_measurement (      )//
237 -* Click "<Call>" next to //prepare_for_measurement//
238 -This will:
239 -** drive the collimator in //IN// position
240 -** drive detector to 200 mm
242 +**Start run in kamzik
243 +**
241 241  
242 -* Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed //
245 +Click "Start" icon in kamzik.
243 243  
244 -//**Set run parameters and go 🏃💥**//
247 +[[image:attach:kamzik_run-tile.jpg||height="400"]]
245 245  
246 -1. Set your desired No. of frames
247 -1. Enter the protein ID of your crystal sample
248 -1. Match exposure time with chopper setting or set desired exposure time
249 -1. Check trigger setting and match to chopper setting
250 -no chopper → external triffer //off//
251 - chopper → external trigger// on//
252 -1. To start the run, click "<Call>" next to //start_run (exposure    )//
253 -{{/layout-cell}}
254 -
255 -{{layout-cell}}
256 -[[image:attach:Kamzik_run(2).PNG]]
257 -{{/layout-cell}}
258 -{{/layout-section}}
259 -
260 -{{layout-section ac:type="single"}}
261 -{{layout-cell}}
262 -(% class="relative-table wrapped" style="width:55.9569%" %)
263 263  |=(((
264 264  Parameter
265 265  )))|=(((
... ... @@ -270,7 +270,7 @@
270 270  |(((
271 271  Images number
272 272  )))|(((
273 -number of frames you want to collect
259 +Set number of frames you want to collect
274 274  )))|(% colspan="1" %)(((
275 275  usually 100k - 200k, but adjust according to hitrate
276 276  
... ... @@ -281,9 +281,12 @@
281 281  )))|(((
282 282  exposure time of Eiger
283 283  
284 -
270 +\\
285 285  )))|(% colspan="1" %)(((
286 -with chopper         → opening time displayed in chopper GUI
272 +chopper at 133 Hz → 3.7 ms
273 +
274 +chopper at 66 Hz   → 7.7 ms
275 +
287 287  no chopper            → 7.7 ms
288 288  
289 289  ----
... ... @@ -293,7 +293,7 @@
293 293  )))|(((
294 294  changes automatically
295 295  )))|(% colspan="1" %)(((
296 -
285 +\\
297 297  )))
298 298  |(% colspan="1" %)(((
299 299  Detector distance
... ... @@ -300,109 +300,66 @@
300 300  )))|(% colspan="1" %)(((
301 301  current detector postion readout
302 302  )))|(% colspan="1" %)(((
303 -
292 +\\
304 304  )))
305 305  |(% colspan="1" %)(((
306 -protein ID
295 +move_detector (distance)
307 307  )))|(% colspan="1" %)(((
308 -protein ID of crystal sample (see AMARCORD)
297 +Set detector distance
309 309  )))|(% colspan="1" %)(((
310 -
299 +200.0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
311 311  )))
301 +|(% colspan="1" %)(((
302 +Diode voltage
303 +)))|(% colspan="1" %)(((
304 +read out of diode
305 +)))|(% colspan="1" %)(((
306 +**~~ 0 !!! higher values indicate missaligned beam stop!!!**
307 +)))
312 312  
313 313  ----
314 -{{/layout-cell}}
315 -{{/layout-section}}
316 316  
317 -{{layout-section ac:type="two_equal"}}
318 -{{layout-cell}}
319 -**After data collection/Before entering the exp. hutch:**
311 +(% class="task-list" %)
312 +(((
313 +{{task reference="/Tasks/Task_6" status="InProgress"}}
314 +Set your desired No. of frames
315 +{{/task}}
320 320  
321 -1. Go to "Safety" in kamzik.
322 -1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
323 -11. Drive the collimator out
324 -11. Drive the detector back to 1500 mm
325 -1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
317 +{{task reference="/Tasks/Task_7" status="InProgress"}}
318 +Match exposure time with chopper setting
319 +{{/task}}
326 326  
327 -[[image:attach:Screenshot from 2022-04-04 19-36-29.png]]
328 -{{/layout-cell}}
321 +{{task reference="/Tasks/Task_8" status="InProgress"}}
322 +Check trigger setting and match to chopper setting
323 +{{/task}}
329 329  
330 -{{layout-cell}}
331 -
325 +{{task reference="/Tasks/Task_9" status="InProgress"}}
326 +Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
327 +{{/task}}
332 332  
329 +{{task reference="/Tasks/Task_10" status="InProgress"}}
330 +To start the run, click "<Call>" next to //exposure time//
331 +{{/task}}
332 +)))
333 333  
334 -
335 -
336 -
337 -[[image:attach:safety_kamzik.PNG]]
338 -{{/layout-cell}}
339 -{{/layout-section}}
340 -
341 -{{layout-section ac:type="single"}}
342 -{{layout-cell}}
343 343  ----
344 344  
345 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7. Troubleshooting =
336 +\\
346 346  
347 -* (((
348 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==
338 +\\
349 349  
350 -* Tape roll empty?
351 -* motor connected (icon green)?
352 -)))
340 +=== {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Troubleshooting"/}}14.8 Troubleshooting ===
353 353  
354 354  * (((
355 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ==
356 -
357 -* displayed in green=connected?
358 -* nozzle holder colliding somewhere?
359 -* motor limits reached? → position displayed in red
343 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
360 360  )))
361 -
362 362  * (((
363 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ==
364 -
365 -* channel turned on?
366 -* Sample empty?
367 -* Nozzle or lines clogged? → flush with water, change nozzle
346 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
368 368  )))
369 -
370 370  * (((
371 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ==
372 -
373 -* First, restart kamzik (server+client) and see if this solves the issue
374 -* If not, restart the Simplon server (in Astor → haspp11exp03, right click on Simplon/EH.1 and restart)
375 -* (((
376 -If nothing works, kill the Simplon process and then start the server again:*
377 -
378 -(((
379 -(% class="code" %)
380 -(((
381 -ssh haspp11exp03
349 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
382 382  )))
383 -)))
384 384  
385 -* (((
386 -(% class="code" %)
387 -(((
388 -ps -ef | grep Simplon
389 -)))
390 -)))
391 -* (((
392 -(% class="code" %)
393 -(((
394 -kill -9 simplon_process_id
395 -)))
396 -)))
397 -)))
398 -* After the Simplon server is back up, close all kamzik windows and restart (first server, then client)
399 -)))
400 -{{/layout-cell}}
401 -{{/layout-section}}
352 +\\
402 402  
403 -{{layout-section ac:type="single"}}
404 -{{layout-cell}}
405 -
406 -{{/layout-cell}}
407 -{{/layout-section}}
408 -{{/layout}}
354 +\\
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1 +https://confluence.desy.de/spaces/11/pages/236824562/14. Serial Crystallography: CFEL TapeDrive
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