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Last modified by chatzies on 2025-11-27 11:35
From version 83.6
edited by tabermah
on 2024-12-10 15:54
Change comment: There is no comment for this version
To version 80.1
edited by tabermah
on 2024-12-10 15:20
Change comment: There is no comment for this version

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... ... @@ -9,13 +9,13 @@
9 9  
10 10  ----
11 11  
12 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =
12 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1 Sample characteristics and preparation =
13 13  
14 -* Suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
14 +suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
15 15  
16 16  Preparation:
17 17  
18 -* filter through 30 µm (green) or 50 µm (yellow) cell filter into an Eppendorf or Falcon (original!) tube
18 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
... ... @@ -22,10 +22,13 @@
22 22  
23 23  ----
24 24  
25 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
25 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2 Connecting sample to ElveFlow, sample exchange =
26 26  
27 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish tube goes all the way to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
27 +*
28 +** ==
28 28  
30 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
31 +
29 29  Be aware to which channel and nozzle line your sample is connected.
30 30  
31 31   Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
... ... @@ -41,10 +41,12 @@
41 41  
42 42  ----
43 43  
44 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3. TapeDrive operation =
47 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3 TapeDrive operation =
45 45  
46 -​​​​​​The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
49 +*
50 +** ==
47 47  
52 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
48 48  Now start the client. Same thing, a terminal will open, shortly after a small window.
49 49  
50 50  Click 'Devices' in the **client session** to open the control window.
... ... @@ -57,10 +57,8 @@
57 57  * Common velocity is 1.0-1.5 mm/s
58 58  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
59 59  
65 +* [[image:attach:kamzik_tape.PNG||height="400"]]
60 60  
61 -[[image:attach:kamzik_tape.PNG||height="400"]]
62 -
63 -
64 64  == {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle ==
65 65  
66 66  * Click on the 'nozzle' icon.
... ... @@ -69,7 +69,7 @@
69 69  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
70 70  
71 71  
72 -(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving the nozzle, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
75 +(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
73 73  \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
74 74  
75 75  (% class="wrapped" %)
... ... @@ -115,10 +115,8 @@
115 115  
116 116  
117 117  )))
121 +* [[image:attach:kamzik_nozzle.PNG||height="400"]]
118 118  
119 -[[image:attach:kamzik_nozzle.PNG||height="400"]]
120 -
121 -
122 122  == {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller ==
123 123  
124 124  * The chiller circulates fluid through the gas nozzle.
... ... @@ -138,7 +138,7 @@
138 138  
139 139  ----
140 140  
141 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4. Tape roll exchange =
142 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4 Tape roll exchange =
142 142  
143 143  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
144 144  
... ... @@ -156,6 +156,8 @@
156 156  
157 157  **[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]**
158 158  
160 +
161 +
159 159  
160 160  {{/layout-cell}}
161 161  {{/layout-section}}
... ... @@ -164,7 +164,7 @@
164 164  {{layout-cell}}
165 165  ----
166 166  
167 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5. Chopper =
170 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5 Chopper =
168 168  
169 169  start chopper gui from terminal:
170 170  
... ... @@ -207,7 +207,7 @@
207 207  {{layout-cell}}
208 208  ----
209 209  
210 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6. Data collection =
213 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6 Data collection =
211 211  
212 212  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
213 213  
... ... @@ -231,7 +231,7 @@
231 231  
232 232  (% style="color:#000000" %)Click "Run" icon in kamzik.
233 233  
234 -* Put detector distance 200 mm in //prepare_for_measurement (      )//
237 +* Put detector distance 200 in //prepare_for_measurement (      )//
235 235  * Click "<Call>" next to //prepare_for_measurement//
236 236  This will:
237 237  ** drive the collimator in //IN// position
... ... @@ -328,6 +328,10 @@
328 328  {{layout-cell}}
329 329  
330 330  
334 +
335 +
336 +
337 +
331 331  [[image:attach:safety_kamzik.PNG]]
332 332  {{/layout-cell}}
333 333  {{/layout-section}}
... ... @@ -336,7 +336,7 @@
336 336  {{layout-cell}}
337 337  ----
338 338  
339 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7. Troubleshooting =
346 += {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7 Troubleshooting =
340 340  
341 341  * (((
342 342  == {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==
... ... @@ -393,4 +393,10 @@
393 393  )))
394 394  {{/layout-cell}}
395 395  {{/layout-section}}
403 +
404 +{{layout-section ac:type="single"}}
405 +{{layout-cell}}
406 +
407 +{{/layout-cell}}
408 +{{/layout-section}}
396 396  {{/layout}}