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Last modified by chatzies on 2025-11-27 11:35
From version 87.1
edited by chatzies
on 2025-11-27 11:32
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To version 77.1
edited by tabermah
on 2024-12-03 11:26
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Title
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1 -Serial Crystallography: CFEL TapeDrive
1 +10. Serial Crystallography: CFEL TapeDrive
Author
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1 -XWiki.chatzies
1 +XWiki.tabermah
Content
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4 4  [[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
5 5  
6 6  
7 -{{toc/}}
7 +{{toc minLevel="2"/}}
8 8  
9 9  
10 10  ----
11 11  
12 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}8.1. Sample characteristics and preparation =
12 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}**14.1 Sample characteristics and preparation** ==
13 13  
14 -* Suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
14 +suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
15 15  
16 16  Preparation:
17 17  
18 -* filter through 30 µm (green) or 50 µm (yellow) cell filter into an Eppendorf or Falcon (original!) tube
18 +* filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
19 19  
20 20  [[image:attach:20211201_095534.jpg||height="250"]]
21 21  
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22 22  
23 23  ----
24 24  
25 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}8.2. Connecting sample to ElveFlow, sample exchange =
25 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}**14.2 Connecting sample to ElveFlow, sample exchange** ==
26 26  
27 -After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish tube goes all the way to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
27 +*
28 +** ==
28 28  
30 +After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
31 +
29 29  Be aware to which channel and nozzle line your sample is connected.
30 30  
31 31   Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
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41 41  
42 42  ----
43 43  
44 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}8.3. TapeDrive operation =
47 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}**14.3 TapeDrive operation** ==
45 45  
46 -​​​​​​The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
49 +*
50 +** ==
47 47  
52 +The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
48 48  Now start the client. Same thing, a terminal will open, shortly after a small window.
49 49  
50 50  Click 'Devices' in the **client session** to open the control window.
51 51  [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
52 52  
53 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}Tape ==
58 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
54 54  
55 55  * Click the 'main tape' icon
56 56  * Change the velocity by typing anything from **0.3-3.0 mm/s**
57 57  * Common velocity is 1.0-1.5 mm/s
58 58  * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
64 +
65 +* [[image:attach:kamzik_tape.PNG||height="400"]]
59 59  
60 -[[image:attach:kamzik_tape.PNG||height="400"]]
67 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
61 61  
62 -
63 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}Sample nozzle ==
64 -
65 65  * Click on the 'nozzle' icon.
66 66  * (((
67 67  The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
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68 68  The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
69 69  
70 70  
71 -(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving the nozzle, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
75 +(% style="color:#ff0000" %)**!!! **(% style="color:#000000" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color:#ff0000" %)** !!!**(%%)
72 72  \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
73 73  
74 74  (% class="wrapped" %)
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112 112  be careful with -z, tape could be pushed out of focus
113 113  )))
114 114  
119 +
115 115  
116 116  )))
122 +* [[image:attach:kamzik_nozzle.PNG||height="400"]]
117 117  
118 -[[image:attach:kamzik_nozzle.PNG||height="400"]]
124 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
119 119  
120 -
121 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}Temperature control/Chiller ==
122 -
123 123  * The chiller circulates fluid through the gas nozzle.
124 124  
125 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}Gas nozzle ==
126 126  
129 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ====
127 127  
128 -== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}IR camera ==
129 129  
132 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ====
130 130  
134 +
131 131  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
132 132  
133 133   xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
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137 137  
138 138  ----
139 139  
140 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}8.4. Tape roll exchange =
144 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}**14.4 Tape roll exchange** ==
141 141  
142 142  The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
143 143  
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155 155  
156 156  **[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]**
157 157  
162 +
163 +
158 158  
159 159  {{/layout-cell}}
160 160  {{/layout-section}}
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163 163  {{layout-cell}}
164 164  ----
165 165  
166 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}8.5. Chopper =
172 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}**14.5 Chopper** ==
167 167  
168 168  start chopper gui from terminal:
169 169  
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175 175  (% class="code" %)
176 176  (((
177 177   python chopperControl.py
184 +\\
178 178  )))
179 179  {{/layout-cell}}
180 180  {{/layout-section}}
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205 205  {{layout-cell}}
206 206  ----
207 207  
208 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}8.6. Data collection =
215 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** ==
209 209  
210 210  Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
211 211  
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229 229  
230 230  (% style="color:#000000" %)Click "Run" icon in kamzik.
231 231  
232 -* Put detector distance 200 mm in //prepare_for_measurement (      )//
239 +* Put detector distance 200 in //prepare_for_measurement (      )//
233 233  * Click "<Call>" next to //prepare_for_measurement//
234 234  This will:
235 235  ** drive the collimator in //IN// position
236 236  ** drive detector to 200 mm
237 237  
245 +
238 238  * Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed //
239 239  
248 +
240 240  //**Set run parameters and go 🏃💥**//
241 241  
242 242  1. Set your desired No. of frames
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326 326  {{layout-cell}}
327 327  
328 328  
338 +
339 +
340 +
341 +
329 329  [[image:attach:safety_kamzik.PNG]]
330 330  {{/layout-cell}}
331 331  {{/layout-section}}
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334 334  {{layout-cell}}
335 335  ----
336 336  
337 -= {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}8.7. Troubleshooting =
350 +== {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}**14.7 Troubleshooting** ==
338 338  
339 339  * (((
340 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ==
353 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
341 341  
342 342  * Tape roll empty?
343 343  * motor connected (icon green)?
344 344  )))
345 345  
359 +
346 346  * (((
347 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ==
361 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
348 348  
349 349  * displayed in green=connected?
350 350  * nozzle holder colliding somewhere?
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351 351  * motor limits reached? → position displayed in red
352 352  )))
353 353  
368 +
354 354  * (((
355 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ==
370 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
356 356  
357 357  * channel turned on?
358 358  * Sample empty?
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359 359  * Nozzle or lines clogged? → flush with water, change nozzle
360 360  )))
361 361  
377 +
362 362  * (((
363 -== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ==
379 +==== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ====
364 364  
365 365  * First, restart kamzik (server+client) and see if this solves the issue
366 366  * If not, restart the Simplon server (in Astor → haspp11exp03, right click on Simplon/EH.1 and restart)
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391 391  )))
392 392  {{/layout-cell}}
393 393  {{/layout-section}}
410 +
411 +{{layout-section ac:type="single"}}
412 +{{layout-cell}}
413 +
414 +{{/layout-cell}}
415 +{{/layout-section}}
394 394  {{/layout}}
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