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Version 46.1 by ahenkel on 2021-12-12 23:53
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ahenkel 37.1 1 {{layout}}
2 {{layout-section ac:type="single"}}
3 {{layout-cell}}
ahenkel 44.1 4 [[image:attach:TapeDrive_beschriftet-tile.jpg||height="250"]]
ahenkel 7.1 5
ahenkel 23.1 6 \\
ahenkel 3.1 7
ahenkel 46.1 8 {{toc minLevel="2"/}}
9
ahenkel 23.1 10 \\
11
ahenkel 45.1 12 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}**14.1 Sample characteristics and preparation** ==
ahenkel 23.1 13
ahenkel 6.1 14 suspension of microcrystals, **at least** 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
ahenkel 5.1 15
16 Preparation:
17
18 * filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube
19
ahenkel 12.1 20 [[image:attach:20211201_095534.jpg||height="250"]]
ahenkel 5.1 21
22 \\
23
ahenkel 45.1 24 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}**14.2 Connecting sample to ElveFlow, sample exchange
25 ** ==
ahenkel 6.1 26
ahenkel 22.1 27 After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
ahenkel 5.1 28
ahenkel 21.1 29 Be aware to which channel and nozzle line your sample is connected.
30
ahenkel 31.1 31 Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
ahenkel 21.1 32
ahenkel 22.1 33 Green → Channel 2, substrate line (nozzle capillary is marked in black)
ahenkel 21.1 34
35 [[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
36
ahenkel 28.1 37 After finishing data collection, flush all lines by connecting an Eppi or syringe filled with ddH,,2,,O.
ahenkel 5.1 38
ahenkel 28.1 39 [[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]]
40
ahenkel 5.1 41 \\
ahenkel 6.1 42
ahenkel 45.1 43 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}**14.3 TapeDrive operation
44 ** ==
ahenkel 6.1 45
ahenkel 19.1 46 The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
ahenkel 14.1 47 Now start the client. Same thing, a terminal will open, shortly after a small window.
ahenkel 16.1 48
49 Click 'Devices' in the **client session** to open the control window.
ahenkel 15.1 50 [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
ahenkel 13.1 51
ahenkel 45.1 52 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
ahenkel 14.1 53
ahenkel 23.1 54 * Click on the 'main tape' icon
55 * Change the velocity by typing anything from **0.3-3.0 mm/s**
56 * Common velocity is 1.0-1.5 mm/s
57 * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
ahenkel 14.1 58 \\
ahenkel 23.1 59 * [[image:attach:kamzik_tape.PNG||height="400"]]
ahenkel 14.1 60
ahenkel 26.1 61 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
ahenkel 23.1 62
63 * Click an the 'nozzle' icon.
64 * (((
ahenkel 19.1 65 The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
ahenkel 17.1 66 The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
67
ahenkel 36.1 68
69 (% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
70 \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
71
ahenkel 44.1 72 (% class="wrapped" %)
ahenkel 17.1 73 |=(((
ahenkel 14.1 74 \\
ahenkel 17.1 75 )))|=(((
76 direction
77 )))|=(((
78 recommended step size
79 for visual adjustment
80 )))|=(% colspan="1" %)(((
81 recommended step size
82 during data collection
83 )))
84 |(((
85 +x
86 )))|(((
ahenkel 18.1 87 away from X-ray interaction point
ahenkel 17.1 88 )))|(((
89 1.0 - 0.5 mm
90 )))|(% colspan="1" %)(((
91 0.1 - 0.2 mm
92 )))
93 |(((
94 +y
95 )))|(((
96 up
97 )))|(((
98 1.0 - 0.5 mm
99 )))|(% colspan="1" %)(((
100 0.1 - 0.2 mm
101 )))
102 |(((
103 +z
104 )))|(((
105 towards detector tower, away from tape
106 )))|(((
107 0.5 - 0.2 mm
108 )))|(% colspan="1" %)(((
109 0.1 - 0.2 mm
110 be careful with -z, tape could be pushed out of focus
111 )))
ahenkel 14.1 112
ahenkel 19.1 113 **
ahenkel 17.1 114 \\**
115 )))
ahenkel 23.1 116 * [[image:attach:kamzik_nozzle.PNG||height="400"]]
ahenkel 17.1 117
ahenkel 23.1 118 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
119
ahenkel 34.1 120 * The chiller circulates fluid through the gas nozzle.
ahenkel 30.1 121
ahenkel 14.1 122 \\
123
ahenkel 32.1 124 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ====
ahenkel 17.1 125
126 \\
127
ahenkel 33.1 128 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ====
129
ahenkel 23.1 130 \\
131
ahenkel 13.1 132 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
133
134 xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
135 password: //written on control laptop (changes every 6 months)
136 //
137
ahenkel 6.1 138 \\
139
ahenkel 7.1 140 \\
ahenkel 6.1 141
ahenkel 45.1 142 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Taperollexchange"/}}**14.5 Tape roll exchange** ==
ahenkel 12.1 143
ahenkel 19.1 144 The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
ahenkel 12.1 145
ahenkel 19.1 146 * Remove the (almost) empty feeder roll by pulling it upwards.
ahenkel 22.1 147 * Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
ahenkel 19.1 148 * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
ahenkel 35.1 149 * Cut the first cm of the new tape roll, directly behind the black sticky part.
150 * Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll.
151 * Open magnetic tape stabilizer on the **left side**. //!!!BEAMSTOP!!!//
152 * Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s).
153 * Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded.
154 * Attach new tape to the empty roll by using some sticky tape.
ahenkel 22.1 155 * Close tape stabilizer.
ahenkel 35.1 156 * Reset tape counter in kamzik by clicking "<Call>" in "Main-Tape".
ahenkel 19.1 157
ahenkel 36.1 158 **[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]
ahenkel 19.1 159 **
160
ahenkel 6.1 161 \\
162
163 \\
ahenkel 7.1 164
ahenkel 44.1 165 === {{id name="14.SerialCrystallography:CFELTapeDrive-"/}}**
166 ** ===
ahenkel 43.1 167 {{/layout-cell}}
168 {{/layout-section}}
ahenkel 28.1 169
ahenkel 43.1 170 {{layout-section ac:type="single"}}
171 {{layout-cell}}
ahenkel 45.1 172 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Chopper"/}}**14.7 Chopper** ==
ahenkel 12.1 173
ahenkel 42.1 174 start chopper gui from terminal:
ahenkel 28.1 175
ahenkel 42.1 176 (% class="code" %)
177 (((
178 cd /gpfs/local/shared/ChopperControl
179 )))
180
181 (% class="code" %)
182 (((
183 python chopperControl.py
184 )))
185 {{/layout-cell}}
186 {{/layout-section}}
187
188 {{layout-section ac:type="two_equal"}}
189 {{layout-cell}}
190 [[image:attach:20211212_233140.jpg||height="250"]]
191 {{/layout-cell}}
192
193 {{layout-cell}}
194 **Chopper → no Chopper**
195
196 * decrease frequency by max. 10 Hz steps\\
197 ** click arrow down 10x, "Set", arrow down 10x, "Set", ....
198 * at frequency = 10 Hz, click "Stop"
199 * set "Hole" to <none> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
200
201 (% style="color: rgb(0,0,0);" %)**no Chopper → Chopper**
202
203 * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
204 * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps
205 ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", .....
206 * (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms//
207 {{/layout-cell}}
208 {{/layout-section}}
209
210 {{layout-section ac:type="single"}}
211 {{layout-cell}}
ahenkel 45.1 212 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.8Datacollection"/}}**14.8 Data collection** ==
ahenkel 42.1 213
ahenkel 36.1 214 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
215
ahenkel 40.1 216 (% class="code" %)
217 (((
218 xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
219 password: written on control laptop (changes every 6 months)
220 )))
ahenkel 36.1 221
222 //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
223
ahenkel 12.1 224 \\
225
ahenkel 40.1 226 Click "Start" icon in kamzik.
227
228 [[image:attach:kamzik_run-tile.jpg||height="400"]]
229
230 ----
231 {{/layout-cell}}
232 {{/layout-section}}
233
234 {{layout-section ac:type="two_equal"}}
235 {{layout-cell}}
ahenkel 36.1 236 **Check list:**
237
ahenkel 40.1 238 (% style="color: rgb(255,0,0);" %)//always after entering the hutch!//
239
ahenkel 36.1 240 * Open BS1  (//CrystalControl)//
241 * Put collimator in (//CrystalControl)//
242 * Check beamstop position = 27 000 (//Atk panel p11/motor/eh.3.09)//
243 * Open fast shutter (//CrystalControl)//
244 * Check diode voltage ~~ 0.0 (//kamzik Run/Diode voltage)//
245 * Close fast shutter (//CrystalControl)//
246
247 \\
248
ahenkel 40.1 249 **Set run parameters and go 🏃💥**
ahenkel 36.1 250
ahenkel 39.1 251 1. Set your desired No. of frames
252 1. Match exposure time with chopper setting
253 1. Check trigger setting and match to chopper setting
254 1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
255 1. To start the run, click "<Call>" next to //exposure time//
ahenkel 40.1 256
257 \\
258
259 (% style="color: rgb(153,51,102);" %)**//Current bug~://**
260
261 (% style="color: rgb(0,0,0);" %)You need to "Stop" run in kamzik manually after all images have been acquired. This moves the shield up and closes the fast shutter. Afterwards you can start a new run by clicking "<Call>".
262
263
264 (% style="color: rgb(0,0,0);" %)Check progress in terminal with:
265
266 (% class="code" %)
267 (((
268 cd /gpfs/current/raw/scan_frames
269 watch "ls -lrt <run number>/ | tail"
ahenkel 41.1 270 \\\\\\\\
ahenkel 40.1 271 )))
ahenkel 37.1 272 {{/layout-cell}}
273
274 {{layout-cell}}
ahenkel 40.1 275 (% class="wrapped relative-table" style="width: 100.0%;" %)
ahenkel 36.1 276 |=(((
277 Parameter
278 )))|=(((
279 explanation
280 )))|=(% colspan="1" %)(((
281 value
282 )))
283 |(((
284 Images number
285 )))|(((
286 Set number of frames you want to collect
287 )))|(% colspan="1" %)(((
288 usually 100k - 200k, but adjust according to hitrate
289
290 orientation: 10 % hitrate needs at least 100k images
291 )))
292 |(((
293 exposure
294 )))|(((
295 exposure time of Eiger
296
297 \\
298 )))|(% colspan="1" %)(((
299 chopper at 133 Hz → 3.7 ms
300
301 chopper at 66 Hz   → 7.7 ms
302
303 no chopper            → 7.7 ms
304
305 ----
306 )))
307 |(((
308 run id
309 )))|(((
310 changes automatically
311 )))|(% colspan="1" %)(((
312 \\
313 )))
314 |(% colspan="1" %)(((
315 Detector distance
316 )))|(% colspan="1" %)(((
317 current detector postion readout
318 )))|(% colspan="1" %)(((
319 \\
320 )))
321 |(% colspan="1" %)(((
322 move_detector (distance)
323 )))|(% colspan="1" %)(((
324 Set detector distance
325 )))|(% colspan="1" %)(((
326 200.0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
327 )))
328 |(% colspan="1" %)(((
329 Diode voltage
330 )))|(% colspan="1" %)(((
331 read out of diode
332 )))|(% colspan="1" %)(((
ahenkel 40.1 333 **~~ 0 !!! higher values indicate missaligned beam stop!!! 
334 Call local contact
335 **
ahenkel 36.1 336 )))
ahenkel 37.1 337 {{/layout-cell}}
338 {{/layout-section}}
ahenkel 36.1 339
ahenkel 37.1 340 {{layout-section ac:type="single"}}
341 {{layout-cell}}
ahenkel 41.1 342 ----
343
ahenkel 45.1 344 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.9Troubleshooting"/}}**14.9 Troubleshooting** ==
ahenkel 29.1 345
346 * (((
347 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
ahenkel 41.1 348
349 * Tape roll empty?
350 * motor connected (icon green)?
ahenkel 29.1 351 )))
ahenkel 41.1 352
353 \\
354
ahenkel 29.1 355 * (((
356 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
ahenkel 41.1 357
358 * displayed in green=connected?
359 * nozzle holder colliding somewhere?
360 * motor limits reached? → position displayed in red
ahenkel 29.1 361 )))
ahenkel 41.1 362
363 \\
364
ahenkel 29.1 365 * (((
366 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
ahenkel 41.1 367
368 * channel turned on?
369 * Sample empty?
370 * Nozzle or lines clogged? → flush with water, change nozzle
ahenkel 29.1 371 )))
ahenkel 37.1 372 {{/layout-cell}}
373 {{/layout-section}}
374 {{/layout}}