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Version 63.1 by ahenkel on 2022-04-19 14:19
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ahenkel 37.1 1 {{layout}}
2 {{layout-section ac:type="single"}}
3 {{layout-cell}}
ahenkel 54.1 4 [[image:attach:TapeDrive_beschriftet-tile.jpg||height="400"]]
ahenkel 7.1 5
ahenkel 23.1 6 \\
ahenkel 3.1 7
ahenkel 46.1 8 {{toc minLevel="2"/}}
9
ahenkel 23.1 10 \\
11
ahenkel 47.1 12 ----
13
ahenkel 45.1 14 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.1Samplecharacteristicsandpreparation"/}}**14.1 Sample characteristics and preparation** ==
ahenkel 23.1 15
ahenkel 55.1 16 suspension of microcrystals, **at least** 200 µL with 20% crystal density (more is always better), size range: 2-30 µm, **tip:** bring some mother liquor for washing, filtering, diluting etc.
ahenkel 5.1 17
18 Preparation:
19
ahenkel 60.1 20 * filter through 30 µm cell filter (green) or 50 µm (yellow) into Eppendorf or Falcon (original!) tube
ahenkel 5.1 21
ahenkel 12.1 22 [[image:attach:20211201_095534.jpg||height="250"]]
ahenkel 5.1 23
24 \\
25
ahenkel 47.1 26 ----
27
ahenkel 45.1 28 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.2ConnectingsampletoElveFlow,sampleexchange"/}}**14.2 Connecting sample to ElveFlow, sample exchange
29 ** ==
ahenkel 6.1 30
ahenkel 22.1 31 After filtration, screw the ElveFlow connector to your Eppendorf or Falcon tube. Make sure that the yellow-ish line goes to the bottom of your tube. You can adjust the length by unscrewing the blue fitting on top and pushing/pulling the yellow-ish line. **Don't forget to tigthen the blue fitting afterwards!**
ahenkel 5.1 32
ahenkel 21.1 33 Be aware to which channel and nozzle line your sample is connected.
34
ahenkel 31.1 35 Red → Channel 1, crystal line (nozzle capillary without marks/with red marks)
ahenkel 21.1 36
ahenkel 22.1 37 Green → Channel 2, substrate line (nozzle capillary is marked in black)
ahenkel 21.1 38
39 [[image:attach:20211201_095033.jpg||thumbnail="true" height="250"]]
40
ahenkel 28.1 41 After finishing data collection, flush all lines by connecting an Eppi or syringe filled with ddH,,2,,O.
ahenkel 5.1 42
ahenkel 28.1 43 [[image:attach:20211201_095232.jpg||height="250"]][[image:attach:20211201_095205.jpg||height="250"]]
44
ahenkel 5.1 45 \\
ahenkel 6.1 46
ahenkel 47.1 47 ----
48
ahenkel 45.1 49 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.3TapeDriveoperation"/}}**14.3 TapeDrive operation
50 ** ==
ahenkel 6.1 51
ahenkel 19.1 52 The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open and shortly after a small window.
ahenkel 14.1 53 Now start the client. Same thing, a terminal will open, shortly after a small window.
ahenkel 16.1 54
55 Click 'Devices' in the **client session** to open the control window.
ahenkel 15.1 56 [[image:attach:screen.PNG||height="250"]][[image:attach:server client.PNG||height="250"]]
ahenkel 13.1 57
ahenkel 45.1 58 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tape"/}}**Tape** ====
ahenkel 14.1 59
ahenkel 63.1 60 * Click the 'main tape' icon
ahenkel 23.1 61 * Change the velocity by typing anything from **0.3-3.0 mm/s**
62 * Common velocity is 1.0-1.5 mm/s
63 * kamzik displays the used tape in m and %. At 98% the tape will automatically stop since the rolls need to be exchanged at this point.
ahenkel 14.1 64 \\
ahenkel 23.1 65 * [[image:attach:kamzik_tape.PNG||height="400"]]
ahenkel 14.1 66
ahenkel 26.1 67 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Samplenozzle"/}}**Sample nozzle** ====
ahenkel 23.1 68
ahenkel 62.1 69 * Click on the 'nozzle' icon.
ahenkel 23.1 70 * (((
ahenkel 19.1 71 The nozzle position can be adjusted in xyz by clicking on the arrows up and down. (Double arrow = 10 steps). Step size can be varied, 1mm steps are rather big and should be used carefully.
ahenkel 17.1 72 The absolute position is **not calibrated** and therefore changes after each restart of kamzik.
73
ahenkel 36.1 74
75 (% style="color: rgb(255,0,0);" %)**!!! **(% style="color: rgb(255, 0, 0); color: rgb(0, 0, 0)" %)**During data collection make sure in which direction you are moving, do not move nozzle into beam**(% style="color: rgb(255,0,0);" %)** !!!**(%%)
76 \\Adjust the nozzle position by eye until it nearly touches the tape and roughly meets the X-ray interaction point. Then, move to the control hutch to use the on-axis microscope for fine adjustment. Move the nozzle tip to the beam position (red X) and write down the absolut nozzle position. From there move the nozzle in +x to your desired distance.Different distances combined with the tape speed result in several delay times for time-resolved experiments.
77
ahenkel 44.1 78 (% class="wrapped" %)
ahenkel 17.1 79 |=(((
ahenkel 14.1 80 \\
ahenkel 17.1 81 )))|=(((
82 direction
83 )))|=(((
84 recommended step size
85 for visual adjustment
86 )))|=(% colspan="1" %)(((
87 recommended step size
88 during data collection
89 )))
90 |(((
91 +x
92 )))|(((
ahenkel 18.1 93 away from X-ray interaction point
ahenkel 17.1 94 )))|(((
95 1.0 - 0.5 mm
96 )))|(% colspan="1" %)(((
97 0.1 - 0.2 mm
98 )))
99 |(((
100 +y
101 )))|(((
102 up
103 )))|(((
104 1.0 - 0.5 mm
105 )))|(% colspan="1" %)(((
106 0.1 - 0.2 mm
107 )))
108 |(((
109 +z
110 )))|(((
111 towards detector tower, away from tape
112 )))|(((
113 0.5 - 0.2 mm
114 )))|(% colspan="1" %)(((
115 0.1 - 0.2 mm
116 be careful with -z, tape could be pushed out of focus
117 )))
ahenkel 14.1 118
ahenkel 19.1 119 **
ahenkel 17.1 120 \\**
121 )))
ahenkel 23.1 122 * [[image:attach:kamzik_nozzle.PNG||height="400"]]
ahenkel 17.1 123
ahenkel 23.1 124 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Temperaturecontrol/Chiller"/}}**Temperature control/Chiller** ====
125
ahenkel 34.1 126 * The chiller circulates fluid through the gas nozzle.
ahenkel 30.1 127
ahenkel 14.1 128 \\
129
ahenkel 32.1 130 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Gasnozzle"/}}**Gas nozzle** ====
ahenkel 17.1 131
132 \\
133
ahenkel 33.1 134 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-IRcamera"/}}**IR camera** ====
135
ahenkel 23.1 136 \\
137
ahenkel 13.1 138 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
139
140 xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
141 password: //written on control laptop (changes every 6 months)
142 //
143
ahenkel 6.1 144 \\
145
ahenkel 7.1 146 \\
ahenkel 6.1 147
ahenkel 47.1 148 ----
ahenkel 12.1 149
ahenkel 47.1 150 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.4Taperollexchange"/}}**14.4 Tape roll exchange** ==
151
ahenkel 19.1 152 The tape stops automatically at 98% used length, if you need to change the rolls for orther reasons, stop the tape (velocity = 0.0 mm/s).
ahenkel 12.1 153
ahenkel 19.1 154 * Remove the (almost) empty feeder roll by pulling it upwards.
ahenkel 22.1 155 * Cut the tape with some cm overlap to glue to the new roll. **Do not discard empty roll!**
ahenkel 19.1 156 * Get a new tape roll (bottom left on the cart). **Polypropylen 15 µm**
ahenkel 35.1 157 * Cut the first cm of the new tape roll, directly behind the black sticky part.
158 * Place new roll on roll holder and use sticky tape to attach the end of the old tape to the beginning of the new roll.
ahenkel 56.1 159 * Open magnetic tape stabilizer on the **left side**. (% style="color: rgb(255,0,0);" %)//!!!BEAMSTOP!!!//
ahenkel 35.1 160 * Start the tape (3mm/s). Once the connetion side has arrived at the collector roll, stop the tape (0mm/s).
161 * Again, cut the tape directly behind the connection side. Replace the the old roll with the **empty **one. Old roll can be discarded.
162 * Attach new tape to the empty roll by using some sticky tape.
ahenkel 22.1 163 * Close tape stabilizer.
ahenkel 35.1 164 * Reset tape counter in kamzik by clicking "<Call>" in "Main-Tape".
ahenkel 19.1 165
ahenkel 36.1 166 **[[image:attach:20211201_094101.jpg||height="250"]][[image:attach:20211201_094111.jpg||height="250"]][[image:attach:tape reset.PNG||height="250"]]
ahenkel 19.1 167 **
168
ahenkel 6.1 169 \\
170
171 \\
ahenkel 7.1 172
ahenkel 48.1 173 **
174 **
ahenkel 43.1 175 {{/layout-cell}}
176 {{/layout-section}}
ahenkel 28.1 177
ahenkel 43.1 178 {{layout-section ac:type="single"}}
179 {{layout-cell}}
ahenkel 47.1 180 ----
ahenkel 12.1 181
ahenkel 47.1 182 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.5Chopper"/}}**14.5 Chopper** ==
183
ahenkel 42.1 184 start chopper gui from terminal:
ahenkel 28.1 185
ahenkel 42.1 186 (% class="code" %)
187 (((
188 cd /gpfs/local/shared/ChopperControl
189 )))
190
191 (% class="code" %)
192 (((
193 python chopperControl.py
ahenkel 47.1 194 \\\\
ahenkel 42.1 195 )))
196 {{/layout-cell}}
197 {{/layout-section}}
198
199 {{layout-section ac:type="two_equal"}}
200 {{layout-cell}}
201 [[image:attach:20211212_233140.jpg||height="250"]]
202 {{/layout-cell}}
203
204 {{layout-cell}}
205 **Chopper → no Chopper**
206
207 * decrease frequency by max. 10 Hz steps\\
208 ** click arrow down 10x, "Set", arrow down 10x, "Set", ....
209 * at frequency = 10 Hz, click "Stop"
210 * set "Hole" to <none> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
211
212 (% style="color: rgb(0,0,0);" %)**no Chopper → Chopper**
213
214 * (% style="color: rgb(0,0,0);" %)set "Hole" to <Inner hole (largest)> (% style="color: rgb(255,0,0);" %)//Takes some minutes//
215 * (% style="color: rgb(0,0,0);" %)increase frequency by max. 10 Hz steps
216 ** (% style="color: rgb(0,0,0);" %)click arrow up 10x, "Set", arrow up 10x, "Set", .....
217 * (% style="color: rgb(0,0,0);" %)//usually 133 Hz, results in exposure time of 3.7 ms//
218 {{/layout-cell}}
219 {{/layout-section}}
220
ahenkel 59.1 221 {{layout-section ac:type="single"}}
ahenkel 42.1 222 {{layout-cell}}
ahenkel 47.1 223 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.6Datacollection"/}}**14.6 Data collection** ==
224
ahenkel 36.1 225 Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:
226
ahenkel 40.1 227 (% class="code" %)
228 (((
229 xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
230 password: written on control laptop (changes every 6 months)
231 )))
ahenkel 36.1 232
ahenkel 51.1 233 //>>usually the terminal is open, just repeat previous command with arrow up, enter and type in the password//
ahenkel 36.1 234
ahenkel 12.1 235 \\
ahenkel 59.1 236 {{/layout-cell}}
237 {{/layout-section}}
ahenkel 12.1 238
ahenkel 59.1 239 {{layout-section ac:type="two_left_sidebar"}}
240 {{layout-cell}}
ahenkel 51.1 241 **Check list:**
ahenkel 40.1 242
ahenkel 51.1 243 (% style="color: rgb(255,0,0);" %)//always after entering the hutch!//
ahenkel 40.1 244
ahenkel 51.1 245 (% style="color: rgb(0,0,0);" %)Click "Safety" icon in kamzik.
246
247 * Put collimator in
248 ** Click "<Call>" next to //collimator_in//
249
250 \\
251
ahenkel 59.1 252 * Open beam shutter BS1 in //CrystalControl //or// P11 XRayFeed //
ahenkel 40.1 253 {{/layout-cell}}
ahenkel 51.1 254
255 {{layout-cell}}
ahenkel 59.1 256 [[image:attach:kamzik_safety.PNG||height="400"]]
ahenkel 51.1 257 {{/layout-cell}}
ahenkel 40.1 258 {{/layout-section}}
259
ahenkel 51.1 260 {{layout-section ac:type="single"}}
ahenkel 40.1 261 {{layout-cell}}
ahenkel 51.1 262 ----
ahenkel 36.1 263
ahenkel 51.1 264 Click "Start/Run" icon in kamzik.
ahenkel 40.1 265
ahenkel 51.1 266 [[image:attach:kamzik_run-tile.jpg||height="400"]]
ahenkel 36.1 267
ahenkel 51.1 268 ----
269 {{/layout-cell}}
270 {{/layout-section}}
ahenkel 36.1 271
ahenkel 51.1 272 {{layout-section ac:type="two_equal"}}
273 {{layout-cell}}
ahenkel 40.1 274 **Set run parameters and go 🏃💥**
ahenkel 36.1 275
ahenkel 39.1 276 1. Set your desired No. of frames
277 1. Match exposure time with chopper setting
278 1. Check trigger setting and match to chopper setting
279 1. Set detector distance to 200.0 mm, confirm with //enter// and click "<Call>" button next to it.
280 1. To start the run, click "<Call>" next to //exposure time//
ahenkel 40.1 281
282 \\
283
ahenkel 51.1 284 \\
ahenkel 40.1 285
ahenkel 52.1 286 **After data collection/Before entering the exp. hutch:**
ahenkel 40.1 287
ahenkel 51.1 288 1. Go to "Safety" in kamzik.
289 1. Click "<Call>" next to //prepare_to_open_hutch. //This will automatically:
290 11. Drive the collimator out
291 11. Drive the detector back to 1500 mm
292 1. Close beam shutter BS1 and break door interlock in //P11 XRayFeed //(monitor top left).
ahenkel 40.1 293
ahenkel 51.1 294 [[image:attach:Screenshot from 2022-04-04 19-36-29.png||height="400"]]
ahenkel 37.1 295 {{/layout-cell}}
296
297 {{layout-cell}}
ahenkel 40.1 298 (% class="wrapped relative-table" style="width: 100.0%;" %)
ahenkel 36.1 299 |=(((
300 Parameter
301 )))|=(((
302 explanation
303 )))|=(% colspan="1" %)(((
304 value
305 )))
306 |(((
307 Images number
308 )))|(((
309 Set number of frames you want to collect
310 )))|(% colspan="1" %)(((
311 usually 100k - 200k, but adjust according to hitrate
312
313 orientation: 10 % hitrate needs at least 100k images
314 )))
315 |(((
316 exposure
317 )))|(((
318 exposure time of Eiger
319
320 \\
321 )))|(% colspan="1" %)(((
322 chopper at 133 Hz → 3.7 ms
323
324 chopper at 66 Hz   → 7.7 ms
325
326 no chopper            → 7.7 ms
327
328 ----
329 )))
330 |(((
331 run id
332 )))|(((
333 changes automatically
334 )))|(% colspan="1" %)(((
335 \\
336 )))
337 |(% colspan="1" %)(((
338 Detector distance
339 )))|(% colspan="1" %)(((
340 current detector postion readout
341 )))|(% colspan="1" %)(((
342 \\
343 )))
344 |(% colspan="1" %)(((
345 move_detector (distance)
346 )))|(% colspan="1" %)(((
347 Set detector distance
348 )))|(% colspan="1" %)(((
349 200.0 mm   (% style="color: rgb(255,0,0);" %)confirm with //enter//, click "<Call>"
350 )))
351 |(% colspan="1" %)(((
352 Diode voltage
353 )))|(% colspan="1" %)(((
354 read out of diode
355 )))|(% colspan="1" %)(((
ahenkel 40.1 356 **~~ 0 !!! higher values indicate missaligned beam stop!!! 
357 Call local contact
358 **
ahenkel 36.1 359 )))
ahenkel 37.1 360 {{/layout-cell}}
361 {{/layout-section}}
ahenkel 36.1 362
ahenkel 37.1 363 {{layout-section ac:type="single"}}
364 {{layout-cell}}
ahenkel 41.1 365 ----
366
ahenkel 47.1 367 == {{id name="14.SerialCrystallography:CFELTapeDrive-14.7Troubleshooting"/}}**14.7 Troubleshooting** ==
ahenkel 29.1 368
369 * (((
370 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Tapestopped"/}}Tape stopped ====
ahenkel 41.1 371
372 * Tape roll empty?
373 * motor connected (icon green)?
ahenkel 29.1 374 )))
ahenkel 41.1 375
376 \\
377
ahenkel 29.1 378 * (((
379 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Nozzlemotorsarenotresponding"/}}Nozzle motors are not responding ====
ahenkel 41.1 380
381 * displayed in green=connected?
382 * nozzle holder colliding somewhere?
383 * motor limits reached? → position displayed in red
ahenkel 29.1 384 )))
ahenkel 41.1 385
386 \\
387
ahenkel 29.1 388 * (((
389 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Sampleflowisnotrespondingtohigherpressure"/}}Sample flow is not responding to higher pressure ====
ahenkel 41.1 390
391 * channel turned on?
392 * Sample empty?
393 * Nozzle or lines clogged? → flush with water, change nozzle
ahenkel 29.1 394 )))
ahenkel 51.1 395
396 \\
397
ahenkel 49.1 398 * (((
399 ==== {{id name="14.SerialCrystallography:CFELTapeDrive-Simplonfilewriter/eigerstreamissues"/}}Simplon filewriter/eiger stream issues ====
400
401 * First, restart kamzik (server+client) and see if this solves the issue
402 * If not, restart the Simplon server (in Astor → haspp11exp03, right click on Simplon/EH.1 and restart)
ahenkel 51.1 403 * (((
404 If nothing works, kill the Simplon process and then start the server again:* (((
405 (% class="code" %)
406 (((
407 ssh haspp11exp03
ahenkel 49.1 408 )))
ahenkel 51.1 409 )))
410 * (((
411 (% class="code" %)
412 (((
413 ps -ef | grep Simplon
414 )))
415 )))
416 * (((
417 (% class="code" %)
418 (((
419 kill -9 simplon_process_id
420 )))
421 )))
422 )))
423 )))
ahenkel 37.1 424 {{/layout-cell}}
425 {{/layout-section}}
ahenkel 51.1 426
427 {{layout-section ac:type="single"}}
428 {{layout-cell}}
429 \\
430 {{/layout-cell}}
431 {{/layout-section}}
ahenkel 37.1 432 {{/layout}}