Changes for page 03a Reconstruction with Reco GUI
Last modified by flenners on 2026-02-04 17:25
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... ... @@ -19,14 +19,26 @@ 19 19 20 20 **HOLOTOMO DATA** 21 21 22 - We are still in"friendlyuseroperation"for this modeand the reconstruction pipelineis stillworkinprogress.22 +Hint: you have to perform the phase reconstruction of your projections first! This is usually done by beamline staff. Then you can continue: 23 23 24 - Update soon ...24 +Go to "Load" tab. Click on "Load normalized data" at the bottom of the page and select your phase reconstruced projections. 25 25 26 - = {{id name="03aReconstructionwithRecoGUI-Preparationofdataforreconstruction"/}}Preparation ofdataforreconstruction =26 +The common path is: ///asap3/petra3/gpfs/p05/YEAR/data/BEAMTIMEID/processed/SCANNAME/reco_~#~#// 27 27 28 +Click on one file and click open 29 + 30 +You do not need to load any additional dark images. 31 + 32 +Skip "Normalize, Minus Log, Rotate" in the Preperation Tab. The optional steps are still optional. (see below) 33 + 34 +In principle, you can directly proceed to the third tab "Reconstruction". 35 + 28 28 \\ 29 29 38 += {{id name="03aReconstructionwithRecoGUI-Preparationofdataforreconstruction"/}}Preparation of data for reconstruction = 39 + 40 +(Can be skipped for Holotomography) 41 + 30 30 Go to "Prep" tab. 31 31 32 32 The following is usually required for reconstruction: ... ... @@ -65,23 +65,94 @@ 65 65 66 66 **Finding the correct center of rotation. ** 67 67 68 -First, you have to find the correct center of rotation. For this, a single slice is reconstructed with different centers of rotation .80 +First, you have to find the correct center of rotation. For this, a single slice is reconstructed with different centers of rotation 69 69 70 -**Rot center:** For the start, enter half of your image size (1024 for unbinned data, 512 for data with binning 2.) 82 +**~1. Rot center: **Defines the rotation center in pixel coordinates. For the start, enter half of your image size (1024 for unbinned data, 512 for data with binning 2.) 71 71 72 -**Delta:** Range which is reconstructed. Good starting value is 50. For fine rot center, choose 10.84 +**2. Delta: **Range of pixels around the in step 1 given rot center for which the test slice is reconstructed. Good starting value is 50. For fine rot center, choose 10. 73 73 74 -**Stepsize:** Stepsize beween the different rotation centers. Good start value is 5 and 1 for fine rot center. 86 +**3. Stepsize:** Stepsize beween the different rotation centers. Good start value is 5 and 1 for fine rot center. (for example [ ... , 507, 512, 517, ... ] for a stepsize of 5) 75 75 76 -Slice: Slice whichwillbe reconstructed. You can also check different slices for checking the rotation center.88 +**4. Slice:** Defines the pixel row which is used to reconstruct the test slice. Tip: Choose a region where you expect to see distinctive structures. (You can also check different slices for checking the rotation center.) 77 77 90 +**5. Check rotation center: **Click on the "Check rotation center" button.** **In the preview window move the slider around until you find the positions with the least artifacts. Remember the position number and check the Spyder Console to get the new rot center coordinates in pixel. 91 + 92 +[[image:attach:image2022-11-14_14-4-49.png||thumbnail="true" height="250"]] 93 + 94 +in blue the coordinate of the center of rotation; in yellow the position number of the preview slider 95 + 96 +**6. Update and Repeat:** Replace the Rot Center (step 1) with the new found coordinate, lower the Delta (step 2) and Stepsize (step 3) and repeat the process until you are satisfied with the result. 97 + 78 78 \\ 79 79 100 +How does a good rotation center look like? 101 + 102 +(% class="relative-table wrapped" style="width: 57.8179%;" %) 103 +|=((( 104 +good 105 +)))|=((( 106 +bad 107 +)))|=((( 108 +comment 109 +))) 110 +|((( 111 +(% class="content-wrapper" %) 112 +((( 113 +[[image:attach:image2022-11-14_14-17-45.png||height="250"]] 114 +))) 115 +)))|((( 116 +(% class="content-wrapper" %) 117 +((( 118 +[[image:attach:image2022-11-14_14-19-33.png||height="250"]] 119 +))) 120 +)))|((( 121 +\\ 122 +))) 123 +|((( 124 +(% class="content-wrapper" %) 125 +((( 126 +[[image:attach:image2022-11-14_15-16-34.png||height="250"]] 127 +))) 128 +)))|((( 129 +(% class="content-wrapper" %) 130 +((( 131 +[[image:attach:image2022-11-14_15-7-13.png||height="250"]] 132 +))) 133 +)))|((( 134 +The ghosting effect on the edges is due to sample movement during the scan. 135 +))) 136 +|((( 137 +\\ 138 +)))|((( 139 +\\ 140 +)))|((( 141 +\\ 142 +))) 143 +|((( 144 +(% class="content-wrapper" %) 145 +((( 146 +[[image:attach:image2022-11-14_15-29-47.png||height="250"]] 147 +))) 148 +)))|((( 149 +(% class="content-wrapper" %) 150 +((( 151 +**[[image:attach:image2022-11-14_15-28-50.png||height="250"]]** 152 +))) 153 +)))|((( 154 +\\ 155 +))) 156 + 157 +[[image:attach:image2023-1-23_11-39-57.png||height="400"]] 158 + 159 +\\ 160 + 161 +\\ 162 + 80 80 **Reconstruction Parameters. ** 81 81 82 82 When you found the correct rotation center, you can continue with the reconstruction. 83 83 84 -Enter the final roation center from the above step. 167 +Enter the final roation center from the above step. 85 85 86 86 You can select different reconstruction algorithms and filters. Standard is gridrec and shepp. 87 87
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... ... @@ -1,1 +1,1 @@ 1 - 2757580091 +361457350 - URL
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... ... @@ -1,1 +1,1 @@ 1 -https://confluence.desy.de/spaces/P5I/pages/ 275758009/03a Reconstruction with Reco GUI1 +https://confluence.desy.de/spaces/P5I/pages/361457350/03a Reconstruction with Reco GUI