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14. Serial Crystallography: CFEL TapeDrive

Version 14.1 by ahenkel on 2021-12-02 09:25

TapeDrive_beschriftet.PNGTapeDrive scheme.png

14.1 Revert from Crystallography to TapeDrive (P11 staff/CFEL)

  • remove vortex
  • beamstop to out-position?
  • remove goniometer. The airbearing should be stopped (p11/servermotor/eh.1.01)
  • move cryojet out of the way, ramp the cryo up and shut it down. Close the compressed air to the shield flow. The biggest cable kinks and needs to be disconnected.
  • stop refill of the robot dewar and empty the dewar, warm up.
  • Disable backlight (unplug Festo connection). Is this still needed?
  • mount the 10 um diode and connect (via connector on the granite). This is connected to the leftmost Keithly (power up, check that settings are gain E06, Filter on, Rise time 1 ms. Keithly is connected to the oscilloscope and the delay generator, delay generator is connected to oscilloscope and detector, see diagram in P11 wiki. Insert here the setting for oscilloscope. Make sure to connect the multi-trigger to the back of the detector instead of the standard trigger, when starting to use the chopper (pull the thin ring with ‘grip’ area to detach). Cables are labeled. Make sure before using the chopper that the channels are enabled.
  • move goniotower to X ~ 17 000

Mounting of the TapeDrive is done by the CFEL group. Electronics and control units are combined on a small shelf, that requires only one power and network plug-in. The shelf will be placed left-sided (looking upstream) to the detector tower. The ElveFlow and control laptop as well as several necessary consumables are placed on a cart, which is to be positioned on the right (looking upstream) against the robot platform. The CFEL chiller is placed on the right of the platform and connected to the TapeDrive GASnozzle.


  • adjust focus of the tape by moving X in goniometer tower until you see it in the overview camera. Final adjustment should be done when there is flow. Latest value is about 4500 in X and also 5000 in Z (higher soft limit changed from 2976,63 to 6000). Better to use the back illumination in the GUI to focus the tape - old instruction, not sure if this is the case. Back illumination is the external light source in the detector tower (the crystallography backlight should be disable for tapedrive).
  • Check the Minimum Detector Distance; the detector should not collide with the tapedrive while driven to 154 mm but it has to be verified. Also check that all cables and capillaries are taped away when the users start, so that nothing protrudes further than the tapedrive itself. Soft limit can be edited in eh.3.11 Atk panel.
  • Reconfirm the alignment of the beamstop, once everything, including the nozzle, is in place. Modify config.ini for beamstop in- and out-position to both mach in (27 000). This way the beamstop will not be driven out when the hutch is opened, to avoid that the users forget to drive it in when restarting after entering the hutch.


Prepare necessary controls for data collection:

  • ATK panels:
    • p11/simplon_filewriter/eh.01
    • p11/simplon_detector/eh.01
      • count time = 0.0075
      • trigger (ints if working without the chopper, # or triggers is 1, exts if with the chopper, # or triggers = number of images). Exts is also the standard trigger in normal crystallography. If GUI is used to take images (e.g. for beamstop alignment) the trigger mode will be forced by that and needs to be reverted manually for the users to continue.
      • numer of images = 100 000
      • number of images in container = 1000
      • mode 16m/4M
      • detector distance = 200 mm (usually)
    • p11/detectortower
    • p11/motor/eh.09
  • remote access to TapeDrive control laptop:
    • open terminal, execute command:          xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
                                                                   password: written on control laptop
  • Cameras:
    • haspp11ethcam1 → detector tower camera to monitor nozzle
    • haspp11ethcam4 →  goniometer camera to monitor tape from above
  • OnDa (on haspp11user04): 
    This starts both GUIs (Frame Viewer & Crystallography GUI) and onda-monitor, the server component. The tool has a bunch of parameters which you can list with ./onda-runner.py —help.
    • 16M mode →    cd /home/p11user/CfelSoft/202103_onda
                               ./onda-runner.py onda-config/onda-config-Eiger16M.toml
    • 4M mode   →    cd /home/p11user/CfelSoft/202103_onda
                               ./onda-runner.py onda-config/onda-config-Eiger4M.toml


14.2 Sample characteristics and preparation

suspension of microcrystals, at least 200 µL with 20% crystal density(more is always better), size range: 2-30 µm, tip: bring some mother liquor for washing, filtering, diluting etc.

Preparation:

  • filter through 30 µm cell filter (green) into Eppendorf or Falcon (original!) tube

20211201_095534.jpg


14.3 Connecting sample to ElveFlow, sample exchange

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14.4 TapeDrive operation

The TapeDrive is controlled through kamzik. To open kamzik, start the server. A terminal will open, shortly after a small window.
Now start the client. Same thing, a terminal will open, shortly after a small window.

  • Tape:
    • Click on the 'main tape' icon
    • Change the velocity by typing anything from 0.3-3.0 mm/s
    • Common velocity is 1.0-1.5 mm/s
    • kamzik_tape.PNG




Once the hutch is interlocked, set remote control to the TapeDrive laptop by opening a terminal and executing the following command:

                                        xfreerdp +clipboard /size:1920x1080 /u:Cfelusr /v:cfeld-pcx22611
                                        password: written on control laptop (changes every 6 months)



14.5 Tape roll exchange

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